A rapid slide agglutination test for the diagnosis of neurocysticercosis in the rural health set up

Biswas, Rakhi; Parija, Subhash Chandra

doi:10.4103/2229-5070.86942

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…Direct agglutination test can be used as an indicator of the presence of antibodies against bacteria. In addition, this method might be appropriate to be used as an of K. pneumoniae antigens and/or antibodies and utilized in disease surveillance/clinical laboratories for rapid identification of pathogens (Danielsson and Kronvall, 1974;Parija and Biswas 2011).…”

Section: Monospecificity Determination Of Antibodiesmentioning

confidence: 99%

Development of monospecific polyclonal antibodies against hypervirulent Klebsiella pneumoniae

et al. 2020

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Abstract. Darniati, Setiyaningsih S, Agungpriyono DR, Handharyani E. 2021. Development of monospecific polyclonal antibodies against hypervirulent Klebsiella pneumoniae. Biodiversitas 22: 99-105. The aim of the research was to produce monospecific antibodies against Klebsiella pneumoniae serotype K1 and K2. Klebsiella pneumonia isolates were recovered from pneumonic lungs of Aceh cattle. The bacteria were identified by rpoB (RNA polymerase β subunit) PCR amplification specific for K. pneumoniae. The presence of capsule cluster gene magA and k2A was used to characterize capsular serotype K1 and K2, respectively. Antisera were produced using serial immunization of New Zealand White rabbits with K. pneumoniae serotypes K1 and K2. The presence of antibodies was determined by using immunodiffusion test and purification was performed by immunoaffinity purification method. K. pneumoniae antibodies began to appear on the 14th day after the first injection and progressively intensified after the 1st and 2nd booster. The cross-reactivity between the antigen was eliminated by absorbing the antisera with the opposite antigen and several serotypes of non-K1/K2. After purification, serum protein concentrations were substantially decreased from 58.48 µg/µL and 53.99 µg/µL to 2.38 µg / µL and 3.72 µg / µL for K1 and K2, respectively). The SDS-PAGE analysis showed two bands with molecular weight at 54 kDa and 25 kDa representing the heavy and light chains of immunoglobulin G, respectively. Purified polyclonal antiseras against K. pneumoniae serotypes K1 and K2 showed high affinity and specificity for homologous antigens in immunodiffusion and direct agglutination tests, and immunohistochemical staining.

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Section: Monospecificity Determination Of Antibodiesmentioning

confidence: 99%

Development of monospecific polyclonal antibodies against hypervirulent Klebsiella pneumoniae

et al. 2020

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“…Simple and rapid latex-based agglutination test for detection of T. solium metacestode antigen in the CSF and serum has been evaluated for diagnosis of NCC exhibiting sensitivity and specificity of 64% and 85% and 52% and 96% in CSF and serum samples, respectively. [ 63 ] Non-invasive samples such as urine and saliva have also been evaluated for antigen detection. Parija et al .…”

Section: Diagnosismentioning

confidence: 99%

Neurocysticercosis: A disease of neglect

2013

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Neurocysticercosis (NCC) is a neglected tropical disease caused by larval forms of the parasite Taenia solium lodging in central nervous system (CNS). There is a huge morbidity and debilitation due to CNS manifestations of NCC in developing and underdeveloped regions of the globe, mainly Asian, African and Latin American countries. It is the cause of epilepsy in about 1% of the population of endemic countries and is the underlying etiology in about 15-50% persons with epilepsy, depending upon the geographical region. There is no perfect diagnostic method and the diagnosis relies on a combination of clinical, radio-imaging, immunologic and epidemiologic data. Treatment includes anti-parasitic treatment by cysticidal drugs and management of associated symptoms and complications. The disease is eradicable and control depends on an integrated and coordinated involvement of international bodies like the World Health Organization along with scientific institutions and political and administrative strata of the endemic countries to provide the essential tools such as adequate sanitation, live-stock management, health education and improved socio-economic conditions.

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“…Latex agglutination test has been the method of choice in the development of a rapid test kit in many fields including clinical and veterinary medicines [ 16 – 20 ]. In principle, latex agglutination test is based on agglutination reactions between antigen and antibody.…”

Section: Introductionmentioning

confidence: 99%

Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications

Mahat

Abdullah

Hussin

2014

Journal of Immunology Research

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Measurement of von Willebrand factor antigen (VWF : Ag) levels is usually performed in a specialised laboratory which limits its application in routine clinical practice. So far, no commercial rapid test kit is available for VWF : Ag estimation. This paper discusses the technical aspect of latex agglutination method which was established to suit the purpose of estimating von Willebrand factor (VWF) levels in the plasma sample. The latex agglutination test can be performed qualitatively and semiquantitatively. Reproducibility, stability, linearity, limit of detection, interference, and method comparison studies were conducted to evaluate the performance of this test. Semiquantitative latex agglutination test was strongly correlated with the reference immunoturbidimetric assay (Spearman's rho = 0.946, P < 0.001, n = 132). A substantial agreement (κ = 0.77) was found between qualitative latex agglutination test and the reference assay. Using the scoring system for the rapid latex test, no agglutination is with 0% VWF : Ag (control negative), 1+ reaction is equivalent to <20% VWF : Ag, and 4+ reaction indicates >150% VWF : Ag (when comparing with immunoturbidimetric assay). The findings from evaluation studies suggest that latex agglutination method is suitable to be used as a rapid test kit for the estimation of VWF : Ag levels in various clinical conditions associated with high levels and low levels of VWF : Ag.

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A rapid slide agglutination test for the diagnosis of neurocysticercosis in the rural health set up

Cited by 5 publications

References 12 publications

Development of monospecific polyclonal antibodies against hypervirulent Klebsiella pneumoniae

Development of monospecific polyclonal antibodies against hypervirulent Klebsiella pneumoniae

Neurocysticercosis: A disease of neglect

Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications

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