Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications

Mahat, Marianor; Abdullah, Wan Zaidah; Hussin, Che Maraina Che

doi:10.1155/2014/850810

Cited by 9 publications

(9 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bead-antigen mixtures were allowed to incubate at room temperature for 2 h. After incubation, plates were microscopically (10X magnification) evaluated for determination of agglutination. Agglutination was recorded on an arbitrary scale of 0-4 with 0 as no visual agglutination and 4 as very strong agglutination (Mahat et al, 2014).…”

Section: Agglutination Assaymentioning

confidence: 99%

Development of a bead-agglutination assay for rapid detection of Tritrichomonas foetus

Schaut

Corbeil

Blake³

et al. 2017

Veterinary Parasitology

View full text Add to dashboard Cite

Tritrichomonas foetus is a flagellated protozoan parasite that causes inflammation of the reproductive tract leading to early embryonic death and abortion in cattle, thereby resulting in significant economic losses. Testing and culling infected bulls is an important strategy for parasite control. Routine testing is mainly limited to bulls that are traveling across state lines or within states that have specific control programs. Both culture and PCR detection methods are available, but they are not typically conducted as part of a yearly breeding soundness program and are not easily conducted in the field. In the present study, we developed a bead agglutination assay for detection of T. foetus antigens. Our experiments revealed that latex beads conjugated to T. foetus lipophosphoglycan-binding antibodies visibly clump in the presence of T. foetus. The detection limit of the assay, determined using both field and laboratory isolates of the parasite, was 0.25μg/mL and 1.0μg/mL total T. foetus antigen, respectively. Our results indicate that an antigen detection test could offer a tool for screening bulls under field conditions.

show abstract

Section: Agglutination Assaymentioning

confidence: 99%

Development of a bead-agglutination assay for rapid detection of Tritrichomonas foetus

Schaut

Corbeil

Blake³

et al. 2017

Veterinary Parasitology

View full text Add to dashboard Cite

show abstract

“…These results raise the possibility that a semiquantitative assay could be developed by configuring cell density scales or coupling with microscopy observations. Some studies have suggested that the agglutination assay is not suitable when the bacterial cell density is very low (Fronczek, ; Mahat, Abdullah, & Che Hussin, ).…”

Section: Discussionmentioning

confidence: 99%

An agglutination test for fast and specific detection of Erwinia psidii

et al. 2019

View full text Add to dashboard Cite

Dieback and wilt, caused by Erwinia psidii (Ep), is one of the most important emergent diseases of Eucalyptus spp. in Brazil. Currently, pathogen detection relies on isolation of bacteria from infected plant tissue and either identification based on morphological, physiological and biochemical tests or DNA amplification using the polymerase chain reaction (PCR), which in many cases is laborious and cumbersome. Considering the need for a simpler and more rapid, yet reliable, method for detecting the pathogen, we obtained a polyclonal antibody (anti‐Ep) and developed an agglutination test for specific detection of E. psidii. The antiserum was produced against the E. psidii strain LPF534 and tested against 101 E. psidii isolates from Eucalyptus spp.; three E. psidii isolates from Psidium guajava; 23 Ralstonia solanacearum and 18 Xanthomonas axonopodis isolates pathogenic to Eucalyptus spp.; and seven endophytic isolates from Eucalyptus spp., three of which are phylogenetically related to the genus Erwinia. Results of direct ELISA indicated that a concentration as low as 3.5 µg/ml of the anti‐Ep antibody was able to detect the E. psidii antigen and that the antibody did not cross‐reacted with other bacteria pathogenic and non‐pathogenic to Eucalyptus spp. In the agglutination test, the anti‐Ep antibody showed positive reaction with all strains of E. psidii tested whereas cross‐reaction with none of the strains that belong to other taxonomic groups was observed. The agglutination test showed a detection limit of 105 colony‐forming units (CFU)/ml, and its specificity was the same as that obtained by PCR amplification using E. psidii‐specific primers. These results demonstrate that the agglutination test developed here is a useful tool for specific, fast and inexpensive detection of E. psidii although only operational on pure bacterial suspensions and not yet directly from infected tissues.

show abstract

“…SARS-CoV-2 antigen-latex particle conjugates were prepared by passive adsorption following the procedures described by Mahat et al 24 , with some modifications. Briefly, 0.4 mL of 5% (w/v) latex suspension was centrifuged at 3,000 g for 5 minutes at room temperature, and the supernatant was discarded.…”

Section: Methodsmentioning

confidence: 99%

Rapid and accurate point-of-care testing for SARS-CoV2 antibodies

Esmail

Knauer

Abdoh

et al. 2020

Preprint

View full text Add to dashboard Cite

The COVID-19 pandemic, caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has grown into worst public health crisis since the 1918 influenza pandemic. As COVID-19 continues to spread around the world, there is urgent need for a rapid, yet accurate antibody test to identify infected individuals in populations to inform health decisions. We have developed a rapid, accurate and cost-effective serologic test based on antibody-dependent agglutination of antigen-coated latex particles, which uses ~5 ul plasma and takes <5 min to complete with no instrument required. The simplicity of this test makes it ideal for point-of-care (POC) use at the community level. When validated using plasma samples that are positive or negative for SARS-CoV-2, the agglutination assay detected antibodies against the receptor-binding domain of the spike (S-RBD) or the nucleocapsid (N) protein of SARS-CoV-2 with 100% specificity and ~98% sensitivity. Furthermore, we found that the strength of the S-RBD antibody response measured by the agglutination assay correlated with the efficiency of the plasma in blocking RBD binding to the angiotensin converting enzyme 2 (ACE2) in a surrogate neutralization assay, suggesting that the agglutination assay may be used to identify individuals with virus-neutralizing antibodies. Intriguingly, we found that >92% of patients had detectable antibodies on the day of positive viral RNA test, suggesting that seroconversion may occur earlier than previously thought and that the agglutination antibody test may complement RNA testing for POC diagnosis of SARS-CoV-2 infection.

show abstract

Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications

Cited by 9 publications

References 33 publications

Development of a bead-agglutination assay for rapid detection of Tritrichomonas foetus

Development of a bead-agglutination assay for rapid detection of Tritrichomonas foetus

An agglutination test for fast and specific detection of Erwinia psidii

Rapid and accurate point-of-care testing for SARS-CoV2 antibodies

Contact Info

Product

Resources

About