A Rapid and Effective Method for Simultaneous Determination of Residual Sulfonamides and Sarafloxacin in Pork and Chicken Muscle by High Performance Liquid Chromatography with Accelerated Solvent Extraction–Solid Phase Extraction Cleanup

Abstract

A rapid and effective high performance liquid chromatographic (HPLC) method with accelerated solvent extraction (ASE)-solid phase extraction cleanup was developed for the determination of sulfonamide and sarafloxacin residues in pork and chicken muscle. Four residues were extracted with acetic acid-acetonitrile at 70°C under 10.3 MPa pressure and 5 min static times with 2 static cycles. The standard curves for the determination of four residues have good linearity (r>0.999). The method limits of quantification (LOQs) were 4-12 μg.kg -1 . Intra-and inter-day precisions (RSDs) were 0.3-1.5% and 0.7-1.8%, respectively. Their average recoveries with four spiked levels ranged from 83% to 108% with the RSD of 0.3-1.5%. This method provides an effective extraction procedure and high sensitivity, and can be applied for the determination of 4 residual drugs in pork and chicken muscle at lower than their MRL levels.

Detection and quantification of multiclass antibiotic residues in poultry products using solid-phase extraction and high-performance liquid chromatography with diode array detection

“…Chicken samples from Baoding, China did not contain sulfadiazine [ 42 ], sulfamerazine and sulfadiazine were also absent in chicken samples from Taiwan [ 43 ] and likewise, samples from Romania [ 38 ]. The aforementioned reports are in contrast to those obtained in the present study where sulfamerazine, sulfamoxole and sulfadiazine were found in varying concentrations and in some instances at levels above the MRLs in the different tissues [ 26 ].…”

Determination of sarafloxacin and its analogues in milk using an enzyme-linked immunosorbent assay based on a monoclonal antibody

“…These ndings are within the range of those reported by others. 5,[56][57][58] Therefore, the developed method is reliable for real sample analyses.…”