Determination of sarafloxacin and its analogues in milk using an enzyme-linked immunosorbent assay based on a monoclonal antibody

Tochi, Bitange Nipa; Peng, Juan; Song, Shanshan; Liu, Liqiang; Kuang, Hua

doi:10.1039/c5ay02702k

Cited by 27 publications

(17 citation statements)

References 51 publications

(74 reference statements)

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“…As a result of the 100-fold dilution, there was consistency in the OD values between the samples and the assay buffer. This technique of reducing matrix effects in milk samples has been applied by Peng, Liu, Kuang, Cui, and Xu (2017) and Tochi et al (2016). In this study, bovine milk samples were spiked with different concentrations (0.0, 20, 40, and 80 ng/mL) of LFLX, NRFLX, and ENX to evaluate the performance of the developed ic-ELISA.…”

Section: Recovery Results Of Spiked Bovine Milk Samples By Ic-elisamentioning

confidence: 99%

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Rapid and sensitive immunoassays for the detection of lomefloxacin and related drug residues in bovine milk samples

Mukunzi

Isanga

Suryoprabowo

et al. 2017

Food and Agricultural Immunology

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The misuse and illegal use of fluoroquinolones (FQs) in animal-based food products have drawn considerable attention in several countries. As a result, there has been an increased demand for efficient detection methods of FQs in food products. In this study, we developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic strip device based on a monoclonal antibody against lomefloxacin (LFLX), a second generation FQ. Ic-ELISA had an IC 50 value of 0.19 ng/mL with a limit of detection of 0.04 ng/mL in 0.01 M phosphate-buffered saline (PBS). The intra-and inter-assay recovery rates of LFLX in bovine milk samples were 98.02-107.40% and 100.65-107.82%. The immunochromatographic assay of LFLX in PBS and spiked bovine milk samples had visual cutoff values at 1 and 5.0 ng/mL, respectively, with significant cross-reactivity with norfloxacin and enoxacin. The developed ic-ELISA and strip method may assist in the detection of FQs in foods.ARTICLE HISTORY

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Section: Recovery Results Of Spiked Bovine Milk Samples By Ic-elisamentioning

confidence: 99%

“…Fluoroquinolones (FQs) are a group of synthetic antibiotics that are used for the prevention and treatment of various infection diseases in clinical medicine and animal husbandry (Park, Kim, Han, & Lee, 2013;Tochi et al, 2016). FQs are subdivided into four different generations.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive immunoassays for the detection of lomefloxacin and related drug residues in bovine milk samples

Mukunzi

Isanga

Suryoprabowo

et al. 2017

Food and Agricultural Immunology

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“…The cell lines with highest titer were used to generate sub-clones, which later were injected intraperitoneally into mice primed with 0.5 ml of paraffin oil for antibody production. The ascites were collected from mice after seven days and purified according to caprylic acid-ammonium sulfate precipitation method as previously described (Kuang et al, 2013;Tochi et al, 2016). The collected monoclonal antibodies were evaluated and quantified; the quantification was done by using the following equation: [X1/ Ab1 = X2/Ab2 (where X1 and Ab1 are, respectively, the concentration and absorbance at 278 nm of immunoglobulin G; and X2 is the unknown concentration and Ab2 the absorbance at 278 nm of the mAb)].…”

Section: Production Of Monoclonal Antibodiesmentioning

confidence: 99%

Development of an immunochromatographic assay for hexestrol and diethylstilbestrol residues in milk

Mukunzi

Tochi

Isanga

et al. 2016

Food and Agricultural Immunology

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Hexestrol (HES) and Diethylstilbestrol (DES) are synthetic hormones, which have been found abuse use in animal-origin food production. We developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immune-chromatographic strip to detect these compounds based on monoclonal antibody. Under optimized conditions, the half-inhibition concentration (IC 50 ) values of ic-ELISA were 0.15 µgL −1 and 0.23 µgL −1 for HES and DES, respectively. Spiked milk samples were analyzed. The recovery of both synthetic hormones in the milk samples was 60.48-102.19% (HES) and 89.34-100.16% (DES). In immunechromatographic assay, the visual cutoff values at 0.5 and 1.0 µgL −1 respectively for HES and DES, which allow us to detect milk samples of a concentration low to 10 µgL −1 for HES and 15 µgL −1 for DES. ARTICLE HISTORY

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“…Globally; however, use of synthetic drugs for animal health and production is facing challenges due to a variety of factors, e.g., because of their high costs (Mondal et al, 2013), general toxicity (Patel et al, 2013), drug residual problems in milk and meat (Elmanama and Albayoumi, 2016;Tochi et al, 2016) and development of drug resistance in ticks (Abbas et al, 2014;Coles and Dryden, 2014;Heath and Levot, 2015;Kumar and Partap, 2015;Muyobela et al, 2015;Vudriko et al, 2016) and GIN (Playford et al, 2014;Alonso-Diaz et al, 2008;Borges et al, 2015;Geurden et al, 2015;Muniz-Lagunes et al, 2015;Ramos et al, 2016). In addition, quality of antiparasitic drugs, particularly in developing countries, has led to attention of the stakeholders to find alternatives, may be as a part of drug resistance management programs (Zaman et al, 2012a,b;Sindhu et al, 2014;Ghosh et al, 2015a;Kumar et al, 2016).…”

Section: Control Of Parasitismmentioning

confidence: 99%

An Overview of Plants with Acaricidal and Anthelmintic Properties

Zaman¹

2017

IJAB

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Geo-climatic and socio-economic conditions provide a favourable environment for parasitic population of livestock in Pakistan. Hard ticks (Ixodidae) and gastrointestinal nematodes pose most serious threats to livestock industry. Stackholders rely on synthetic drugs to control these parasites. Emergence of drug resistance in these parasites; however, has provoked interest in alternate of synthetic drugs. Testing of plants used in ethnoveterinary medicine for their antiparasitic activity employing standard procedures has been reported to be promising. Plants have been most frequently used for deworming purposes followed by as acaricides and insecticides; whereas, reports as to their antiprotozoal use are relatively less. Use of plants as antiparasitics is more frequent in developing countries having low accessability to the modern parasite control practices. Likewise, validation studies on the use of plants as antiparasitics have been more frequently carried out in the developing countries compared with those having livestock farming as commercial enterprise. This article presents an overview of the plants having acaricidal and anthelmintic activity, limitations as to the application of phytotherpay to control parasites and future prospects of the use of plants as antiparasitics.

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Determination of sarafloxacin and its analogues in milk using an enzyme-linked immunosorbent assay based on a monoclonal antibody

Abstract: A heterologous immunoassay has been developed for the determination of sarafloxacin (SRFX) and its analogue residues in milk.

Cited by 27 publications

References 51 publications

Rapid and sensitive immunoassays for the detection of lomefloxacin and related drug residues in bovine milk samples

Rapid and sensitive immunoassays for the detection of lomefloxacin and related drug residues in bovine milk samples

Development of an immunochromatographic assay for hexestrol and diethylstilbestrol residues in milk

An Overview of Plants with Acaricidal and Anthelmintic Properties

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