Rapid and sensitive immunoassays for the detection of lomefloxacin and related drug residues in bovine milk samples

Mukunzi, Daniel; Isanga, Joel; Suryoprabowo, Steven; Liu, Liqiang; Kuang, Hua

doi:10.1080/09540105.2017.1306495

Cited by 27 publications

(11 citation statements)

References 28 publications

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“…[13][14][15][16] IL-FST have been reported on the detection of aminoglycosides residues, but these have been limited to the detection of a single analyte. [17][18][19] Simultaneous detection of multiaminoglycosides with an IL-FST represents a considerable challenge because of the structural similarity of these compounds and the difficulty of producing sufficiently specic monoclonal antibodies. In this study we present a novel format of an immunochromatographic lateral ow strip for rapid, simultaneous, semi-quantitative and quantitative detection of GM, NEO, and KN in milk samples based on the use of three different highly specic monoclonal antibodies.…”

Section: Introductionmentioning

confidence: 99%

Development of an immunochromatographic lateral flow strip for the simultaneous detection of aminoglycoside residues in milk

Sun

Yang

et al. 2018

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Development of an immunochromatographic lateral flow strip for the simultaneous detection of aminoglycoside residues in milk

Sun

Yang

et al. 2018

RSC Adv.

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“…To make icELISA work at optimal conditions, some influence parameters were optimized as below 22,23 . Regarding to the optimal combination of coating antigen and mAb, a checkerboard titration was performed in icELISA (see Supplementary Table S1), and the results showed that the dilution ratios of coating antigen (1 mg mL −1 ) and mAb 4B3 (1.0 mg mL −1 ) were at 1:500 and 1:2000, respectively.…”

Section: Resultsmentioning

confidence: 99%

A monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for flubendiamide detection

Cui

Liao

et al. 2019

Sci Rep

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Flubendiamide (FD), the first commercial phthalic acid diamide that targets insect ryanodine receptor (RyRs), has played an important role in pest management. With its extensive worldwide application, a rapid and convenient method to detect its existence in the environment is necessary. In this study, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed to analyse FD residue on environmental and food samples. The established icELISA showed a half maximal inhibition concentration (IC50) of 17.25 µg L−1, with a working range of 4.06–103.59 µg L−1 for FD, and showed no cross-reactivity with chlorantraniliprole, cyantraniliprole, and several FD analogues. Average FD recoveries from spinach, tap water, and soil samples were 89.3–112.3%, 93.0–102.1%, and 86.9–97.6%, respectively. Meanwhile, FD detection results of icELISA were compared with those of ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The comparable results verified that icELISA was suitable for rapid detection of FD residue in environmental and agricultural samples.

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“…To determine polyclonal and monoclonal antibody specificities, antibody cross-reactivity (CR) with other oestrogen analogues, including E 2 benzoate, oestrone, oestriol, diethylstilbestrol, quinestrol, ethinyloestradiol, E 2 valerate, and nonylphenol, were determined. CR values were calculated according to the following equation (Chen, Xu, et al, 2014;Mukunzi, Isanga, Suryoprabowo, Liu, & Kuang, 2017): CR(%) = (IC 50 E 2 /IC 50 analogue compounds) × 100%.…”

Section: Cross-reactivitymentioning

confidence: 99%

Production of antibodies and development of an enzyme-linked immunosorbent assay for 17β-estradiol in milk

Bai

Liu

et al. 2017

Food and Agricultural Immunology

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The residue of 17β-estradiol (E 2) in milk could potentially lead to the occurrence of various reproductive diseases; therefore, a rapid and sensitive method for monitoring E 2 residues in milk was highly necessary. In this study, we produced new polyclonal and monoclonal antibodies using E 2-3-O-carboxymethyl ether as a hapten and developed an indirect competitive enzyme-linked immunosorbent assay (icELISA) for the detection of E 2 in milk. The results showed that the sensitivity of polyclonal antibody was higher than that of monoclonal antibody, providing a half maximum inhibition concentration (IC 50) against E 2 of 0.17 ng/mL, high cross-reactivity (CR) to E 2 benzoate (150%) and oestriol (18.02%), and negligible CR with other oestrogen compounds. Under optimized conditions, the developed icELISA based on the polyclonal antibody had a limit of detection values of 0.093 μg/L, which was enough sensitive to detect E 2 in milk. In spiked samples (0.5, 1, and 2 μg/L), the recoveries ranged from 83.12% to 94.58% with coefficients of variation <12.8%. These results indicated that the icELISA method we developed was suitable for screening of E 2 residue in milk.

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Rapid and sensitive immunoassays for the detection of lomefloxacin and related drug residues in bovine milk samples

Cited by 27 publications

References 28 publications

Development of an immunochromatographic lateral flow strip for the simultaneous detection of aminoglycoside residues in milk

Development of an immunochromatographic lateral flow strip for the simultaneous detection of aminoglycoside residues in milk

A monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for flubendiamide detection

Production of antibodies and development of an enzyme-linked immunosorbent assay for 17β-estradiol in milk

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