Development of an immunochromatographic lateral flow strip for the simultaneous detection of aminoglycoside residues in milk

Sun, Yong; Yang, Jifei; Yang, Suzhen; Sang, Qingbo; Teng, Maikun; Li, Qingmei; Deng, Ruiguang; Li, Feng; Hu, Xiaofei; Zhang, Gaiping

doi:10.1039/c8ra01116h

Cited by 31 publications

(24 citation statements)

References 27 publications

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“…A shortcoming of fluorescence nanoparticles is that they require using an external device for the excitation, so colored nanoparticles that can be visualized by the naked eye have been investigated more extensively. Therefore, the approach based on drawing two or more lines in a single strip and using GNP as the label has been more largely reported [7][8][9][10][11][12][13][14][15][16][17]. In particular, the approach has been applied for the simultaneous detection of food contaminants, such as mycotoxins [7,13,16,17], antibiotics [18,9,12], and pesticides [10,14,15].…”

Section: One-strip Xlfia: Spatial Separation Of Detection Sitesmentioning

confidence: 99%

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Multiplex Lateral Flow Immunoassay: An Overview of Strategies Towards High-Throughput Point-of-Need Testing

Anfossi¹,

Nardo²,

Cavalera³

et al. 2018

Preprint

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Simultaneous measurement of different substances from a single sample is an emerging issue to achieve efficient and high-throughput detection in several fields of application. Although immunoanalytical techniques have well-established and prevailing advantages over alternative screening analytical platforms, one of the incoming challenges for immunoassay is exactly multiplexing. The Lateral Flow Immunoassay (LFIA) is a leading immunoanalytical technique for onsite analysis thanks to its simplicity, rapidity and cost-effectiveness. Moreover, LFIA architecture is adaptable to multiplexing and therefore candidates as a possible answer to the pressing demand of multiplexing point-of-need analysis. The review present an overview of diverse approaches for multiplex LFIA, with a special focus on strategies based on new types of magnetic, fluorescent and colored labels

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Section: One-strip Xlfia: Spatial Separation Of Detection Sitesmentioning

confidence: 99%

“…The maximum number of lines that have been included in a single strip is six (comprising the Control line), which means that up to five different compounds were detected simultaneously [8,14,15]. Most frequently, two-three analytes are detected by one-strip xLFIAs [7,9,10,11,12,13,16,17].…”

Section: One-strip Xlfia: Spatial Separation Of Detection Sitesmentioning

confidence: 99%

Multiplex Lateral Flow Immunoassay: An Overview of Strategies Towards High-Throughput Point-of-Need Testing

Anfossi¹,

Nardo²,

Cavalera³

et al. 2018

Preprint

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“…Competitive format immunochromatographic strip assay based on the specific binding of antibody and antigen possesses distinct benefits for rapidly screening small analytes . Its user‐friendly format, simple analysis and low cost make it commonly used in real‐time detection of veterinary drug residues . To the best of our knowledge, only one paper has reported an application for NIT detection based on the immunological method.…”

Section: Introductionmentioning

confidence: 99%

“…[19][20][21][22][23] Its user-friendly format, simple analysis and low cost make it commonly used in real-time detection of veterinary drug residues. [24][25][26][27][28] To the best of our knowledge, only one paper has reported an application for NIT detection based on the immunological method. Traynor et al 1 developed an immunoassay based on a sensor chip for the measurement of NIT in milk; the biosensor displayed a capability of detecting NIT at less than 10 μg kg −1 in bovine milk.…”

Section: Introductionmentioning

confidence: 99%

A rapid colloidal gold‐based immunochromatographic strip assay for monitoring nitroxynil in milk

Yang

et al. 2020

J Sci Food Agric

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BACKGROUND Nitroxynil (NIT) is a veterinary drug against hepatic fluke disease for food‐producing cattle and sheep. NIT has a long half‐life time in animals since it is highly bound to plasma protein. Therefore NIT possibly remains in animal edible tissues or milk due to drug abuse. In this study, a specific murine monoclonal antibody (mAb) against NIT was prepared and an immunochromatographic strip assay based on the mAb was developed for screening NIT in milk. RESULTS The affinity constant of the anti‐NIT mAb was 2.93 × 1010 and the anti‐NIT mAb had almost no cross‐reactivity with other analogs, so that it showed good specificity. The cutoff value of this test strip was considered to be 50 ng mL−1 by the naked eye. When detected by the strip reader, the half maximum inhibition concentration (IC50) of the immunoassay strip was calculated to be 5.716 ng mL−1 and the limit of detection (LOD) was 1.146 ng mL−1. Intra‐assay recoveries from 88.80 to 97.13% were obtained, with the highest coefficient of variation (CV) at 9.01%; inter‐assay recoveries ranged from 84.60 to 106.87%, with the highest CV at 9.93%. CONCLUSION The operative procedure of the proposed method can be completed within 10 min. The strip developed in this study was a practical tool for rapid semiquantitative and quantitative detection of NIT in milk. This study suggested great potential for analytically monitoring NIT in other food samples. © 2019 Society of Chemical Industry

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“…Immunochemical methods, especially colloidal gold immunochromatographic strips (ICS), are receiving increasing attention due to their convenient and practicality [27,28]. At present, many test strips have been reported on the detection of various veterinary drugs and feed additives, including aminoglycoside antibiotic [29]. sulfonamides antibacterial agents [30], vancomycin [31], paromomycin [32], polymyxin B [33] and sildena l [34].…”

Section: Introductionmentioning

confidence: 99%

An Ultrasensitive Immunoassay Strip for Simultaneously Detecting Cyproheptadinehydrochloride and Six Phenothiazinesin Feedstuffs Based on a Monoclonal Antibody

Na¹,

Hu²,

Sun³

et al. 2020

Preprint

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Background: An antihistamine cyproheptadine (CPH) and phenothiazines (PZs)sedative hypnoticshave a similar tricyclic structure, these drugs are often illegally added to food animal feedstuffs due to their price advantage and significant effect in promoting growth and improving meat quality. However, the abuse of these drugs may lead totheir residues in animal products, thereby causing harmful effects such as allergies and dermatological reactions on human health.To supervise the use of prohibited drugs and ensure food safety, it is necessary to establish a simple and effective screening method to detect CPH and PZs.In this research, an artificial antigen against cyproheptadine(CPH) was successfully synthesized by bromoacetic acid substitution method. An ultrasensitive and broad-specific monoclonal antibody (mAb) able to recognize CPH and six phenothiazines (PZs) was produced. Based on the gold-labeled mAb, an immunochromatographic strip (ICS) was established.Results:The 50% inhibition concentration (IC50) of the produced mAb against CPH was identified as 0.036 ng mL-1 by ELISA, and the cross-reactivities for six PZs were from 6.33% to 63.16%. The visual detection limits(cut-off values) of the developed ICS ranged from 5 to 100ngg-1 in feedstuffs. Take a reading by strip reader, the IC50 was from 0.570 to 7.750ng g-1. In addition,a recovery experiment was carried out to verify the reliability of the ICS. The intra-assay recoveries were from 79.83% to 103.38% with the highest coefficient of variation (CV) of 11.31%. The inter-assay recoveries were from 79.00% to 96.63% with the highest CV of 12.66%.Conclusions: We have successfully produced a broad-spectrum monoclonal antibody and established an ICS for simultaneouslydetecting CPH and six PZs drugs.In brief, the proposed ICS was considered suitable for qualitatively and quantitatively monitoring CPH and PZs in feedstuffs.

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Development of an immunochromatographic lateral flow strip for the simultaneous detection of aminoglycoside residues in milk

Abstract: A colloidal gold-based immunochromatographic strip has been developed for the rapid, simultaneous, semi-quantitative and quantitative detection of several aminoglycoside residues in milk, including gentamicin sulfate (GM), neomycin sulfate (NEO) and kanamycin sulfate (KN).

Cited by 31 publications

References 27 publications

Multiplex Lateral Flow Immunoassay: An Overview of Strategies Towards High-Throughput Point-of-Need Testing

Multiplex Lateral Flow Immunoassay: An Overview of Strategies Towards High-Throughput Point-of-Need Testing

A rapid colloidal gold‐based immunochromatographic strip assay for monitoring nitroxynil in milk

An Ultrasensitive Immunoassay Strip for Simultaneously Detecting Cyproheptadinehydrochloride and Six Phenothiazinesin Feedstuffs Based on a Monoclonal Antibody

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