A putative endocrine factor SIBD (single insulin binding domain protein) involved in immune response of Chinese mitten crab Eriocheir sinensis

Insulin-like growth factor binding protein 7, a member of insulin-like growth factor signal pathway, involved in immune response of small abalone Haliotis diversicolor

“…Similar to EsSIBD and LvSIBD, which were up-regulated at 3 h and 1.5 h after bacterial challenge, respectively [20,21], we found that saIGFBP7 was up-regulated significantly (P < 0.05) at 3 h after exposure to bacteria in gills, then it restored basal level at 6 h, 12 h and 24 h. In hemocytes, there was no significant change at first 3 h post-bacterial challenge. However, at 6 h and 12 h post-injection the transcripts level was significantly increased (P < 0.05).…”

“…Furthermore, in arthropod, it is worth noting here that two single insulin binding domain (SIBD) proteins were identified in Litopenaeus vannamei and Eriocheir sinensis hemocytes cDNA library, respectively. These proteins, which are likely to be a member of the IGFBP superfamily, are participated in the shrimp and crab immune responses by bacterial challenge [20,21]. In addition, a decrease of the temporal SIBD-1 expression was detected in hemocytes after a mixture of Escherichia coli and Staphylococcus aureus was injected into spider Cupiennius salei [22].…”

A Crayfish Insulin-like-binding Protein

“…Thus, our findings present exciting evidence adding new perspective to studies on the early evolution of the IGFBP family, which currently focus mainly on vertebrates (46). It should be emphasized that previous studies in decapods have linked these binding proteins to immune responses (22,23) rather than to reproduction and growth. With the present findings, Cq-IGFBP joins the list of other arthropod IGFBP homologs, namely Imp-L2 and Sf-IBP, previously reported in the fruit fly Drosophila melanogaster and the moth Spodoptera frugiperda (Sf), respectively (35,47).…”

“…Indeed, only a limited number of the conserved components from ILP growth pathways have been reported in crustaceans. These include two IGFBP homologs, although neither is linked to sexuality or growth regulation of the IGF-like AG hormone, instead being found to be associated with immunological responses (22,23). In the current study we report the identification of the first IGFBP homolog to be fully sequenced in the crayfish C. quadricarinatus, namely the organism in which the first decapod IAG transcript was discovered (14).…”

Purification, cDNA structure and biological significance of a single insulin-like growth factor-binding domain protein (SIBD-1) identified in the hemocytes of the spider Cupiennius salei

“…Recently, additional members of the evolutionarily conserved Cys-rich IGFBP/IGFPB-rP superfamily have not only been reported for vertebrates but also for various arthropods such as ticks (Mulenga and Khumthong, 2010), shrimps (Castellanos et al, 2008), crabs (Gai et al, 2010), crayfish , and insects (Alic and Partridge, 2008;Andersen et al, 2000;Honegger et al, 2008). With perlustrin, a first homolog protein to the vertebrate IGFBP family, an N-terminal module was isolated from the shell of the abalone Haliotis laevigata, exhibiting binding of human IGF-I, IGF-II, and bovine insulin (Weiss et al, 2001(Weiss et al, , 2000.…”