A proposal of multiplace hyperbaric chamber for animal experimentation and veterinary use

Rech, Fabrício Valandro; Fagundes, Djalma José; Hermanson, Reginaldo; Rivoire, Henri Chaplin; Fagundes, Anna Luiza Negrini

doi:10.1590/s0102-86502008000400014

Cited by 9 publications

(5 citation statements)

References 14 publications

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“…HBO procedure was carried out in a hyperbaric chamber for experimental animals[13] of University Regional do Alto Uruguai Campus Erechim (URI). Before pressurization, 100% medical oxygen was flushed through the chamber for 5 min to displace the room air.…”

Section: Methodsmentioning

confidence: 99%

Effects of hyperbaric oxygen and N-acetylcysteine in survival of random pattern skin flaps in rats

Rocha

Fagundes

Pires

et al. 2012

Indian J Plast Surg

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Objective:Our aim is to investigate the role of HBO (hyperbaric oxygen), NAC (N-acetylcysteine), and HBO plus NAC on the necrosis area of random rat's skin flaps of a modified McFarlane flap design.Materials and Methods:Thirty-two male Wistar rats were randomly divided into four groups: G-S (sham: n = 8), G-NAC (NAC: n = 8), G-HBO (HBO: n = 8), and G-HN (HBO plus NAC: n = 8). A rectangular skin flap (2 × 8 cm2) was dissected from the muscular dorsal layer, preserving the cranial pedicle. Polyethylene film was placed over the muscular layer and an interrupted 3.0 nylon suture was employed to fix the flap into the original place. On the eighth day, full-thickness biopsy samples (2 × 1 cm2) were collected from the proximal, middle, and cranial areas of the skin flap, and in a site away from the flap labelled as the control area.Results:The measurements of necrotic areas in the groups were 18.3% in G-S, 24.3% in G-NAC, 12.6% in G-HBO, and 14.9% in G-HN. Significant difference was observed between the groups G-HBO and G-HN as well as G-NAC.Conclusion:HBO is associated with reduced area of necrosis of skin flap. The G-NAC group was associated with poor results when examined in isolation. The association between HBO and NAC did not produce favourable results with respect to the use of HBO alone. These findings suggest that the diffusion of oxygen through the interstitial space was the determining factor of more favourable results of HBO.

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Section: Methodsmentioning

confidence: 99%

Effects of hyperbaric oxygen and N-acetylcysteine in survival of random pattern skin flaps in rats

Rocha

Fagundes

Pires

et al. 2012

Indian J Plast Surg

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“…The hyperbaric oxygenation (HBO) was carried out in an appropriate chamber for animal experimentation, developed by the team involved in this research 6 . Before final pressurization, oxygen injected into the chamber for 5 min displaced the environment air inside the camera and allowed a 100% atmosphere of oxygen.…”

Section: Hyperbaric Chambermentioning

confidence: 99%

“…Ten animals, in individual stalls for each section, stayed under continuous oxygen flow. Each daily section lasted two hours during seven consecutive days, always performed at the same hour 6 .…”

Section: Procedures Of Hyperbaric Oxygenationmentioning

confidence: 99%

Action of hyperbaric oxygenation in the rat skin flap

et al. 2015

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PURPOSE:To evaluate the morphology, necrotic area and collagen content in skin flaps of rats subjected to hyperbaric oxygenation (HBO). METHODS:Forty adult rats were divided into four groups: GEC -epilated; GE/HBO -epilated subjected to HBO; GER -epilated submitted to skin flap (2 cm in width /8 cm length in the dorsal area) and GER/HBO -epilated, subjected to skin flap and HBO. HBO (2.4 ATA) was performed for two hours during seven consecutive days. In the eighth day, the rats were anesthetized and the skin flaps were removed and separated into three portions, relative to pedicle fixation. The material fixed in 10% formalin was processed for paraffin embedding; sections were stained by H.E and subjected to picrosirius-red method. The slides examined under light microscopy for evaluation of the collagen content in polarized light microscope and ImageLab ® software (Bio-Rad). RESULTS:The data showed larger area of necrosis and lower levels of collagen in the three regions of the GER group, whereas in the GER/HBO group the collagen content was similar to the GEC and GE/HBO groups. CONCLUSION:Hyperbaric oxygenation reduced the area of necrosis and preserved the morphology and collagen content in skin flaps of rats.

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“…To the authors' knowledge, only RECH et al 7 have recently published a similar technical note on a hyperbaric oxygen chamber for animal use. Such an HBO chamber needs to comply with strict safety regulations, because high pressure and pure oxygen are involved.…”

Section: Discussionmentioning

confidence: 99%