A hyperbaric oxygen chamber for animal experimental purposes

Djasim, Urville M.; Spiegelberg, Linda; Wolvius, Eppo B.; Wal, K.G.H. van der

doi:10.1016/j.ijom.2011.10.007

Cited by 8 publications

(6 citation statements)

References 9 publications

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“…Treatment with hyperbaric oxygen started at day 5 after tumor implantation and consisted of daily sessions, until the end of the experiment with a maximum of 30 sessions. The hyperbaric oxygen chamber used in this study was custom-built for small laboratory animals (Hytech BV, Raamsdonksveer, The Netherlands) [ 24 ]. Each session started with a compression phase of 15 min, during which the pressure in the chamber was elevated to 2.4 atm absolute (ATA) and the oxygen level to 100 %.…”

Section: Methodsmentioning

confidence: 99%

Optical Imaging of Tumor Response to Hyperbaric Oxygen Treatment and Irradiation in an Orthotopic Mouse Model of Head and Neck Squamous Cell Carcinoma

et al. 2015

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PurposeHyperbaric oxygen therapy (HBOT) is used in the treatment of radiation-induced tissue injury but its effect on (residual) tumor tissue is indistinct and therefore investigated in this study.ProceduresOrthotopic FaDu tumors were established in mice, and the response of the (irradiated) tumors to HBOT was monitored by bioluminescence imaging. Near infrared fluorescence imaging using AngioSense750 and Hypoxisense680 was applied to detect tumor vascular permeability and hypoxia.ResultsHBOT treatment resulted in accelerated growth of non-irradiated tumors, but mouse survival was improved. Tumor vascular leakiness and hypoxia were enhanced after HBOT, whereas histological characteristics, epithelial-to-mesenchymal transition markers, and metastatic incidence were not influenced.ConclusionsSquamous cell carcinoma responds to HBOT with respect to tumor growth, vascular permeability, and hypoxia, which may have implications for its use in cancer patients. The ability to longitudinally analyze tumor characteristics highlights the versatility and potential of optical imaging methods in oncological research.Electronic supplementary materialThe online version of this article (doi:10.1007/s11307-015-0834-8) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Optical Imaging of Tumor Response to Hyperbaric Oxygen Treatment and Irradiation in an Orthotopic Mouse Model of Head and Neck Squamous Cell Carcinoma

et al. 2015

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“…The control group received normoxia at sea level pressure. During HBOT, animals were single housed in cages [7] . Control rats were placed next to the machine to experience analogous stress created by the noise of the machinery and breathed normoxia at normal pressure.…”

Section: Methodsmentioning

confidence: 99%

Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats

et al. 2014

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Wound healing in diabetes is frequently impaired and its treatment remains a challenge. Hyperbaric oxygen therapy (HBOT) receives a wide attendance and is often used as a last resort treatment option, however, its effectiveness for many conditions is unproven. We tested the effect of HBOT on healing of diabetic ulcers in an animal experimental setting. Experimental diabetes was induced by intraperitoneal injection of streptozotocin. Four weeks after diabetes induction, rats were ulcerated by clamping a pair of magnet disks on the dorsal skin for 16 h. After magnet removal, the animals received HBOT, daily on weekdays, for 4 weeks. To examine the effect of HBOT on diabetes impaired wound healing, the degree of wound tissue perfusion, inflammation, angiogenesis, and tissue breaking strength were evaluated. HBOT effects on the degree of inflammation and number of blood vessels could not be observed. HBOT improved the tissue breaking strength of the wound, however, this did not reach statistical significance. Twenty hours after ending the HBOT, a significantly improved oxygen saturation of the hemoglobin at the venous end of the capillaries and the quantity of hemoglobin in the micro-blood vessels was measured.

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“…Within each group the animals were divided into the HBO-treated group and the control group, all having comparable glucose levels and body weight. HBO-treated rats were given 100% oxygen under a pressure of 2.4 ATA for 90 min, using a custom designed hyperbaric oxygen tank (IHC High Tech, Raamsdonkveer, the Netherlands) suitable for animals [11]. Non-HBOT (control) rats experienced similar handling and similar machine noise as the HBOT animals, but breathed normoxia at sea level pressure.…”

Section: Methodsmentioning

confidence: 99%

Hyperbaric oxygen therapy for wound healing in diabetic rats: Varying efficacy after a clinically-based protocol

et al. 2017

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Hyperbaric oxygen therapy (HBOT) is a clinical treatment in which a patient breathes pure oxygen for a limited period of time at an increased pressure. Although this therapy has been used for decades to assist wound healing, its efficacy for many conditions is unproven and its mechanism of action is not yet fully clarified. This study investigated the effects of HBOT on wound healing using a diabetes-impaired pressure ulcer rat model. Seven weeks after streptozotocin-induced diabetes in rats (n = 55), a pressure ulcer was created on dorsal skin. Subsequently, animals received HBOT during 6 weeks following a standard clinical protocol (HBOT group with varying endpoints up to 42 days post-wounding) versus controls without HBOT. Capillary venous oxygen saturation (SO2) showed a significant increase in the HBOT group on day 24; however, this increase was significant at this time point only. The quantity of hemoglobin in the micro-blood vessels (rHB) showed a significant decrease in the HBOT group on days 21 and 42, and showed a trend to decrease on day 31. Blood flow in the microcirculation showed a significant increase on days 17, 21 and 31 but a significant decrease on days 24 and 28. Inflammation scoring showed significantly decreased CD68 counts in the HBOT group on day 42, but not in the early stages of wound healing. Animals in the HBOT group showed a trend for an increase in mean wound breaking strength on day 42.

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A hyperbaric oxygen chamber for animal experimental purposes

Cited by 8 publications

References 9 publications

Optical Imaging of Tumor Response to Hyperbaric Oxygen Treatment and Irradiation in an Orthotopic Mouse Model of Head and Neck Squamous Cell Carcinoma

Optical Imaging of Tumor Response to Hyperbaric Oxygen Treatment and Irradiation in an Orthotopic Mouse Model of Head and Neck Squamous Cell Carcinoma

Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats

Hyperbaric oxygen therapy for wound healing in diabetic rats: Varying efficacy after a clinically-based protocol

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