A pollen allergen-encoding gene is expressed in wheat ovaries

Balzer, Hans‐Jörg; Borisiuk, Ljudmilla; Meyer, Hans‐Martin; Matzk, Fritz; Bäumlein, Helmut

doi:10.1007/bf00019095

Cited by 5 publications

(2 citation statements)

References 37 publications

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“…Likewise, a gene encoding a receptor-like kinase is also uniquely expressed in this tissue. In addition, genes that encode pollen allergen-related protein [ 47 , 48 ] and proteinase inhibitor [ 49 ] were expressed in the spike at flowering. All of these data suggest that selection for recognition between male and female gametes, modulation of nucleic acids, stress responses and signal transduction in the field during pollination might operate on these genes.…”

Section: Resultsmentioning

confidence: 99%

Assessment of adaptive evolution between wheat and rice as deduced from full-length common wheat cDNA sequence data and expression patterns

et al. 2009

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BackgroundWheat is an allopolyploid plant that harbors a huge, complex genome. Therefore, accumulation of expressed sequence tags (ESTs) for wheat is becoming particularly important for functional genomics and molecular breeding. We prepared a comprehensive collection of ESTs from the various tissues that develop during the wheat life cycle and from tissues subjected to stress. We also examined their expression profiles in silico. As full-length cDNAs are indispensable to certify the collected ESTs and annotate the genes in the wheat genome, we performed a systematic survey and sequencing of the full-length cDNA clones. This sequence information is a valuable genetic resource for functional genomics and will enable carrying out comparative genomics in cereals.ResultsAs part of the functional genomics and development of genomic wheat resources, we have generated a collection of full-length cDNAs from common wheat. By grouping the ESTs of recombinant clones randomly selected from the full-length cDNA library, we were able to sequence 6,162 independent clones with high accuracy. About 10% of the clones were wheat-unique genes, without any counterparts within the DNA database. Wheat clones that showed high homology to those of rice were selected in order to investigate their expression patterns in various tissues throughout the wheat life cycle and in response to abiotic-stress treatments. To assess the variability of genes that have evolved differently in wheat and rice, we calculated the substitution rate (Ka/Ks) of the counterparts in wheat and rice. Genes that were preferentially expressed in certain tissues or treatments had higher Ka/Ks values than those in other tissues and treatments, which suggests that the genes with the higher variability expressed in these tissues is under adaptive selection.ConclusionWe have generated a high-quality full-length cDNA resource for common wheat, which is essential for continuation of the ongoing curation and annotation of the wheat genome. The data for each clone's expression in various tissues and stress treatments and its variability in wheat and rice as a result of their diversification are valuable tools for functional genomics in wheat and for comparative genomics in cereals.

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Section: Resultsmentioning

confidence: 99%

Assessment of adaptive evolution between wheat and rice as deduced from full-length common wheat cDNA sequence data and expression patterns

et al. 2009

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“…The pollen-specific expression of these proteins implied a possible function in pollen-stigma interaction or pollen tube growth. However, recently expression of a Tri a 3 transcript, showing a high degree of homology to group 2 and 3 grass pollen allergens, was found in the parenchymatous tissue of wheat ovaries, exclusively at the day of anthesis in male fertile wheat lines [13].…”

Section: Cellular Localization and Biological Functions Of Grass Pollmentioning

confidence: 99%

Grass pollen allergens: new developments

Wissenbach

Holm

Neerven

et al. 1998

Clin Experimental Allergy

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RFLP mapping of the dwarfing (Ddw1) and hairy peduncle (Hp) genes on chromosome 5 of rye (Secale cereale L.)

Korzun

Börner

Melz³

1996

Theoret. Appl. Genetics

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An F2 population was established for mapping the two dominant genes for dwarfness (Ddw1) and hairy peduncle (Hp) on chromosome 5R. The location of both genes was shown to be on the segment of chromosome 5RL which was ancestrally translocated and is homoeologous to Triticeae 4L. Hp cosegregated with the wheat gDNA probe WG199, localised in wheat on chromosomes 5AL, 4BL and 4DL. No segregation was observed between the traits hairy peduncle and hairy leaf sheath. The locus for Ddw1 was found to map distally to Hp/Xwg199 but proximal to the isozyme marker β-amy-R1. The genetical distances were 5.6 cM between Hp/Xwg199 and Ddw1 and ll.ScM between Ddw1 and β-amy-R1, respectively. The map position of Ddw1 suggests that it is homoeologous to the wheat dominant dwarfing gene Rht12, present on chromosome 5AL and linked to β-amy-A1.

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A pollen allergen-encoding gene is expressed in wheat ovaries

Cited by 5 publications

References 37 publications

Assessment of adaptive evolution between wheat and rice as deduced from full-length common wheat cDNA sequence data and expression patterns

Assessment of adaptive evolution between wheat and rice as deduced from full-length common wheat cDNA sequence data and expression patterns

Grass pollen allergens: new developments

RFLP mapping of the dwarfing (Ddw1) and hairy peduncle (Hp) genes on chromosome 5 of rye (Secale cereale L.)

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