RFLP mapping of the dwarfing (Ddw1) and hairy peduncle (Hp) genes on chromosome 5 of rye (Secale cereale L.)

Korzun, Viktor; Börner, Andreas; Melz, G.

doi:10.1007/bf00224051

Cited by 58 publications

(26 citation statements)

References 18 publications

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“…Several genes determining reduced plant height, vernalization response, selfincompatibility and a range of morphological mutants have been mapped successfully (Plaschke et al 1993(Plaschke et al , 1995bKorzun et al 1996Korzun et al , 1997Senft and Wricke 1996;Fig. 1 (continued) Voylokov et al 1998).…”

Section: Discussionmentioning

confidence: 96%

Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags

et al. 2004

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The genetic map of rye contains predominantly restriction fragment length polymorphism (RFLP) markers but also a limited number of microsatellite markers, which are known to be more reliable and easier to apply. We report here the saturation of the genomic map of rye with additional microsatellite-derived markers that we obtained from the rye expressed sequence tag (EST) databases and the Gatersleben collection of wheat microsatellite markers (WMS). A total of 99 loci (39 EST and 60 WMS) were mapped into the RFLP frameworks of four rye mapping populations consisting of 139, 64, 58 and 60 RFLPs, respectively. For another ten WMS loci, which amplified PCR products not polymorphic in any of the mapping populations, chromosome and chromosome arm locations were determined using wheat-rye addition lines. Chromosomes 1R, 2R, 3R, 4R, 5R, 6R and 7R were enriched with 9, 19, 9, 13, 27, 16 and 16 microsatellite loci, respectively. The microsatellite loci mapped were evenly distributed along the chromosomes, which is important for the further application of these markers for gene mapping or diversity studies in rye. Forty-four of the WMS loci mapped in rye were found to be homologous to those mapped in bread wheat ( Triticum aestivum L.).

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Section: Discussionmentioning

confidence: 96%

Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags

et al. 2004

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“…On chromosome 5R in population D, the major dwarfing gene Ddw1 is located (25), explaining 72% of phenotypic variation for plant height and having large effects on heading stage (64%) and FHB index (40%). This gene is GA sensitive (27) and, thus, not comparable with RhtB1 and RhtD1 dwarfing genes from wheat in this respect. A second QTL on chromosome 6A also affected all three traits in population C. An association between FHB resistance and plant height for a QTL with a similar map position was previously found by Haeberle et al (21), reporting that the resistance-QTL allele reduced FHB index by 27% but increased plant height by 14 cm.…”

Section: Discussionmentioning

confidence: 87%

Genetic Architecture of Fusarium Head Blight Resistance in Four Winter Triticale Populations

Kalih¹,

Maurer²,

Miedaner³

2015

Phytopathology®

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Fusarium head blight (FHB) is a devastating disease that causes significant reductions in yield and quality in wheat, rye, and triticale. In triticale, knowledge of the genetic architecture of FHB resistance is missing but essential due to modern breeding requirements. In our study, four doubled-haploid triticale populations (N=120 to 200) were evaluated for resistance to FHB caused by artificial inoculation with Fusarium culmorum in four environments. DArT markers were used to genotype triticale populations. Seventeen quantitative trait loci (QTL) for FHB resistance were detected across all populations; six of them were derived from rye genome and located on chromosomes 4R, 5R, and 7R, which are here reported for the first time. The total cross-validated ratio of the explained phenotypic variance for all detected QTL in each population was 41 to 68%. In all, 17 QTL for plant height and 18 QTL for heading stage were also detected across all populations; 3 and 5 of them, respectively, were overlapping with QTL for FHB. In conclusion, FHB resistance in triticale is caused by a multitude of QTL, and pyramiding them contributes to higher resistance.

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“…Ddw1 was originally named as Humilus (Hl, Sturm and Engel 1980), and it is located on the long arm of 5R, between the genes for hairy peduncle (Hp1) and b-amylase . The two closest markers found near Ddw1 are the wheat RFLP marker Xwg199 and the isozyme marker b-amy-R1 (Börner et al 1999;Korzun et al 1996). As the bestknown short straw mutant, EM-1 has been included in many Eastern European, as well as Finnish, rye breeding programs to prevent lodging in rye (Milach and Federizzi 2001).…”

Section: Introductionmentioning

confidence: 99%

Tagging the dwarfing gene Ddw1 in a rye population derived from doubled haploid parents

Tenhola-Roininen

Tanhuanpää

2009

Euphytica

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To prevent lodging and sprouting damage in rye, a dominant dwarfing gene, Ddw1, has been incorporated into breeding lines to reduce plant height in Finland. However, the inability to identify heterozygous plants makes it difficult to purify breeding lines. Doubled haploidy (EM-1 9 Voima) and bulked segregant analysis were used to search for an efficient PCR-based tool to identify homozygous short plants. In addition, SNP (single nucleotide polymorphism) markers were created from the endosperm-specific b-amylase gene and the microsatellite locus REMS1218 expressed in rye and known to be located near Ddw1. The best marker was a combination of the microsatellite REMS1218 and the SNP created from it, located 13 cM from the QTL corresponding to Ddw1. However, for a rye breeder, the SNP alone is an adequate tool to identify plants homozygous for the EM-1 allele and it can be used in selection for the desirable growth habit in rye.

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RFLP mapping of the dwarfing (Ddw1) and hairy peduncle (Hp) genes on chromosome 5 of rye (Secale cereale L.)

Cited by 58 publications

References 18 publications

Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags

Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags

Genetic Architecture of Fusarium Head Blight Resistance in Four Winter Triticale Populations

Tagging the dwarfing gene Ddw1 in a rye population derived from doubled haploid parents

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