A photo-electrochemical aptasensor for the determination of severe acute respiratory syndrome coronavirus 2 receptor-binding domain by using graphitic carbon nitride-cadmium sulfide quantum dots nanocomposite

Abstract

Graphical abstract Schematic diagram of the response mechanism of PEC detection of Sars-Cov-2-RBD by using Aptamer/Chitosan/CdS QDs-gC 3 N 4 /ITO electrode

The role of electrochemical biosensors in SARS-CoV-2 detection: a bibliometrics-based analysis and review

“…In addition to conventional electrochemical signals, photoelectrochemical signals can also be used for detection. Tabrizi et al 160 assembled an aptamer-based photoelectrochemical biosensor to detect S-protein RBD. The photogenerated current was used as the detection signal and could be detected linearly in the range of 0.5–32.0 nM, with a detection limit of 0.12 nM.…”

An electrochemical membrane-based aptasensor for detection of severe acute respiratory syndrome coronavirus-2 receptor-binding domain

“… Aptasensor Biomarkers Technique Linear range Limit of detection Incubation time Ref. SPCE/Aunano/MPA/ biotinylated aptamer SARS-CoV-2-RBD DPV 10–50 ng/mL 2.63 ng/mL 40 min [61] CB/CSPE combined with Magnetic bead-based immunosensor SARS-CoV-2 nucleocapsid proteinSpike protein DPVDPV 0.01–0.6 µg/mL0.04–10 µg/mL 8 ng/mL19 ng/mL 30 min30 min [62] Pad/Aptamer SARS-CoV-2 nucleocapsid protein SPR 0.5–16 ng/mL 1 ng/mL 110 min [63] Gold electrode/Aptamer-Methylene blue SARS-CoV-2-RBD SWV 10 11 -10 10 – 5 min [2] GCE/Yb-TCPP-4/ Au NPs/ Aptamer Spike protein PEC 0.5–8 μg/mL 72 ng/mL 70 min [64] ITO electrode/CdS QDs- Chitosan/gC 3 N 4 Aptamer SARS-CoV-2-RBD PEC 0.5–32.0 nM 0.12 nM 40 [13] LEGE/NPAOM-Si-S-Au nano -Aptamer SARS-CoV-2-RBD SWV 2.5–40.0 ng/mL(71.5 pM-1.14 nM) 0.8 ng/mL(23 pM) 20 min This work DPV: Differential pulse voltammetry; SPR: Surface plasmon resonance; PEC: photoelectrochemistry; MPA: 3-Mercaptopropionic acid; Cu(OH) 2 NRs: Copper hydroxide nanorods; GCE: Glassy carbon electrode; Au NPs: Gold nanoparticles; Yb-TCPP-4: Two-dimensional metal–organic framework …”

“…Hence, several research groups have been working to fabricate the biosensors to detect the SARS-CoV-2 in the early stage of COVID-19. The spike protein [2] , [3] , [4] , [5] , nucleocapsid protein [6] , [7] , [8] , a short fragment of its gene [9] , [10] , [11] , and receptor-binding domain (RBD) [12] , [13] , [14] of the SARS-CoV-2 are the common biomarkers that were detected in the real samples to diagnose the COVID-19.…”

Multifunctional carbon nanomaterials for diagnostic applications in infectious diseases and tumors

“…gC 3 N 4 and fullerenes are also used to construct PEC sensors. Tabrizi et al constructed a PEC aptasensor for SARS-CoV-2 RBD detection with chitosan/cadmium sulfide (CdS)-gC 3 N 4 -modified ITO electrode [ 91 ]. Visible light-driven gC 3 N 4 with a band gap of 2.69 eV compensates for the high recombination rate of photogenerated electrons and holes in CdS quantum dots, while the binding of SARS-CoV-2 RBD to the aptamer in the sensor hinders the natural diffusion of ascorbic acid acting as an electron donor (PEC signal off).…”