An electrochemical membrane-based aptasensor for detection of severe acute respiratory syndrome coronavirus-2 receptor-binding domain

Tabrizi, Mahmoud Amouzadeh; Acedo, Pablo

doi:10.1016/j.apsusc.2022.153867

Cited by 15 publications

(7 citation statements)

References 64 publications

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“…The three-dimensional (3D) image and surface profile were obtained from a two-dimensional (2D) image by the imageJ software. The LEGE and the flow cell fabrication process have been reported in our previous work ( Amouzadeh Tabrizi and Acedo, 2022b ). The geometric surface area (A gsa ) of the working electrode was 0.125 cm 2 .…”

Section: Methodsmentioning

confidence: 99%

Highly sensitive aptasensor for the detection of SARS-CoV-2-RBD using aptamer-gated methylene blue@mesoporous silica film/laser engraved graphene electrode

Tabrizi

Acedo²

2022

Biosensors and Bioelectronics

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Section: Methodsmentioning

confidence: 99%

Highly sensitive aptasensor for the detection of SARS-CoV-2-RBD using aptamer-gated methylene blue@mesoporous silica film/laser engraved graphene electrode

Tabrizi

Acedo²

2022

Biosensors and Bioelectronics

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“…The majority of aptamer‐based biosensor comprises foodborne pathogens, especially Salmonella and E. coli , even though there are different kinds of pathogens rather than foodborne ones. Besides, there were aptamer‐based biosensor studies based on detection of viruses, such as norovirus, 58–62 hepatitis B virus, 63,64 influenza virus, 65–70 and of course SARS‐CoV‐2 71,72 …”

Section: Electrochemical Aptasensors For Pathogenic Point‐of‐care Dia...mentioning

confidence: 99%

“…Besides, there were aptamer-based biosensor studies based on detection of viruses, such as norovirus, [58][59][60][61][62] hepatitis B virus, 63,64 influenza virus, [65][66][67][68][69][70] and of course SARS-CoV-2. 71,72 Salmonella is a rod-shaped, gram-negative, nonspore forming, motile, and one of the most known foodborne pathogen bacterium that is member of Enterobacteriaceae. 73 The bacteria is one of the major worldwide concerns intensified by nearly 95 million foodborne illness and 160,000 deaths per year.…”

Section: Electrochemical Aptasensors For Pathogenic Point-of-care Dia...mentioning

confidence: 99%

Electrochemical aptasensors for pathogenic detection toward point‐of‐care diagnostics

Kızılkurtlu

Demirbaş

Ağel

2023

Biotech and App Biochem

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A biosensor system refers to a biomedical device, which detects biological, chemical, or biochemical components by converting those signals to an electrical signal by utilizing and uniting physical or chemical transducer with biorecognition elements. An electrochemical biosensor is generally based on the reaction of either production or consumption of electrons under a three‐electrode system. Biosensor systems are exploited in a wide range of areas, such as medicine, agriculture, husbandry, food, industry, environment protection, quality control, waste disposal, and the military. Pathogenic infections are the third leading cause of death worldwide after cardiovascular diseases and cancer. Therefore, there is an urgent need for effective diagnostic tools to control food, water, and soil contamination result in protecting human life and health. Aptamers are peptide or oligonucleotide‐based molecules that show very high affinity to their targets that are produced from large pools of random amino acid or oligonucleotide sequences. Generally, aptamers have been utilized for fundamental sciences and clinical implementations for their target‐specific affinity and have been intensely exploited for different kinds of biosensor applications for approximately 30 years. The convergence of aptamers with biosensor systems enabled the construction of voltammetric, amperometric, and impedimetric biosensors for the detection of specific pathogens. In this review, electrochemical aptamer biosensors were evaluated by discussing the definition, types, and production techniques of aptamers, the advantages of aptamers as a biological recognition element against their alternatives, and a wide range of aptasensor examples from literature in the detection of specific pathogens.

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“…Laser engraved graphene electrode modified with electrochemicaly fabricated nanoporous anodic oxide of aluminium membrane (NPAOM) functionalized with 3‐mercaptopropyl trimethoxysilane (NPAOM‐Si‐SH) which was bound to gold nanoparticles via thiol linkage and further with thiolated aptamer was used as the sensing probe. The binding of RBD protein was perceived by analyzing the reduction in the signal of [Fe(CN) 6 ] 3−/4− using SWV [76] …”

Section: Voltammetric Sensors For Diagnosis Of Sars‐cov‐2 and Its Bio...mentioning

confidence: 99%

Voltammetric Sensors: A Versatile Tool in COVID‐19 Diagnosis and Prognosis

2023

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The recent events of outbreaks of corona virus caused by the Severe Acute Respiratory Syndrome Coronavirus −2(SARS‐CoV‐2) raised a concern and increased urge for quick and rapid detection techniques of disease causing pathogens. The detection of the virus with high sensitivity and accuracy holds great importance. Nowadays, the most commonly employed technique is the Quantitative Reverse Transcription Polymerase Chain Reaction test (qRT‐PCR) and antigen test. While RT‐PCR technique is time consuming, expensive and labour intensive, antigen tests though simple, can often give false positive and false negatives. In this context, electrochemical biosensors developed in recent times have been identified as a potential strategy to overcome the limitations of these common techniques. This review article summarizes the current advancements in the field of voltammetric sensors for the detection of COVID‐19 virus and various biomarkers associated with it.

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An electrochemical membrane-based aptasensor for detection of severe acute respiratory syndrome coronavirus-2 receptor-binding domain

Cited by 15 publications

References 64 publications

Highly sensitive aptasensor for the detection of SARS-CoV-2-RBD using aptamer-gated methylene blue@mesoporous silica film/laser engraved graphene electrode

Highly sensitive aptasensor for the detection of SARS-CoV-2-RBD using aptamer-gated methylene blue@mesoporous silica film/laser engraved graphene electrode

Electrochemical aptasensors for pathogenic detection toward point‐of‐care diagnostics

Voltammetric Sensors: A Versatile Tool in COVID‐19 Diagnosis and Prognosis

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