Highly sensitive aptasensor for the detection of SARS-CoV-2-RBD using aptamer-gated methylene blue@mesoporous silica film/laser engraved graphene electrode

Tabrizi, Mahmoud Amouzadeh; Acedo, Pablo

doi:10.1016/j.bios.2022.114556

Cited by 16 publications

(8 citation statements)

References 40 publications

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“…Moreover, the detection of bacterial infections using aptamer probe‐gated SNPs was also reported in samples of food, environmental surfaces, and live organisms [21–26] . In another study, as a viral infection, CoV‐2 was determined using aptamer‐gated methylene blue@mesoporous silica film/laser engraved graphene electrode as a LOD 0.36 ng/mL [27] . Furthermore, single‐stranded oligonucleotide‐gated systems target various molecules such as small molecules like ATP, [28] mRNA, [29] and genomic DNA [19] .…”

Section: Introductionmentioning

confidence: 94%

“…[21][22][23][24][25][26] In another study, as a viral infection, CoV-2 was determined using aptamer-gated methylene blue@mesoporous silica film/laser engraved graphene electrode as a LOD 0.36 ng/mL. [27] Furthermore, singlestranded oligonucleotide-gated systems target various molecules such as small molecules like ATP, [28] mRNA, [29] and genomic DNA. [19] In this context, recently, we reported the potential usage of SNPs in proof-of-concept design for the detection of CoV-2 viral infection using both synthetic DNA and human samples.…”

Section: Introductionmentioning

confidence: 99%

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Direct Detection of Viral Infections from Swab Samples by Probe‐Gated Silica Nanoparticle‐Based Lateral Flow Assay

Durdabak,

Dogan,

Tekol

et al. 2023

ChemistryOpen

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Point‐of‐care diagnosis is crucial to control the spreading of viral infections. Here, universal‐modifiable probe‐gated silica nanoparticles (SNPs) based lateral flow assay (LFA) is developed in the interest of the rapid and early detection of viral infections. The most superior advantage of the rapid assay is its utility in detecting various sides of the virus directly from the human swab samples and its adaptability to detect various types of viruses. For this purpose, a high concentration of fluorescein and rhodamine B as a reporting material was loaded into SNPs with excellent loading capacity and measured using standard curve, 4.19 μmol ⋅ g−1 and 1.23 μmol ⋅ g−1, respectively. As a model organism, severe acute respiratory syndrome coronavirus‐2 (CoV‐2) infections were selected by targeting its nonstructural (NSP9, NSP12) and envelope (E) genes as target sites of the virus. We showed that NSP12‐gated SNPs‐based LFA significantly outperformed detection of viral infection in 15 minutes from 0.73 pg ⋅ mL−1 synthetic viral solution and with a dilution of 1 : 103 of unprocessed human samples with an increasing test line intensity compared to steady state (n=12). Compared to the RT‐qPCR method, the sensitivity, specificity, and accuracy of NSP12‐gated SNPs were calculated as 100 %, 83 %, and 92 %, respectively. Finally, this modifiable nanoparticle system is a high‐performance sensing technique that could take advantage of upcoming point‐of‐care testing markets for viral infection detections.

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Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Direct Detection of Viral Infections from Swab Samples by Probe‐Gated Silica Nanoparticle‐Based Lateral Flow Assay

Durdabak,

Dogan,

Tekol

et al. 2023

ChemistryOpen

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“…An aptasensor was created by Tabrizi et al [64] to identify the RBD of the SARS-CoV-2 virus using methylene blue (MB) encapsulated in a mesoporous silica film (MPSF). Aminofunctionalized MPSF was electrochemically synthesized on the LEGE surface using silicon precursor and a template molecule (cetyltrimethylammonium bromide).…”

Section: Using Graphenementioning

confidence: 99%

Graphene-Based Electrochemical Nano-Biosensors for Detection of SARS-CoV-2

Sengupta¹,

Hussain

2023

Inorganics

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COVID-19, a viral respiratory illness, is caused by Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2), which was first identified in Wuhan, China, in 2019 and rapidly spread worldwide. Testing and isolation were essential to control the virus’s transmission due to the severity of the disease. In this context, there is a global interest in the feasibility of employing nano-biosensors, especially those using graphene as a key material, for the real-time detection of the virus. The exceptional properties of graphene and the outstanding performance of nano-biosensors in identifying various viruses prompted a feasibility check on this technology. This paper focuses on the recent advances in using graphene-based electrochemical biosensors for sensing the SARS-CoV-2 virus. Specifically, it reviews various types of electrochemical biosensors, including amperometric, potentiometric, and impedimetric biosensors, and discusses the current challenges associated with biosensors for SARS-CoV-2 detection. The conclusion of this review discusses future directions in the field of electrochemical biosensors for SARS-CoV-2 detection, underscoring the importance of continued research and development in this domain.

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“…The growth of vertically ordered mesoporous silica nanochannel film (VMSF) on the electrode surface is an important approach for constructing nanostructured electrodes [ 33 , 34 , 35 , 36 ]. Currently, large-area VMSF-modified electrodes (e.g., tens of square centimeters) can be conveniently prepared using the Stöber solution growth method [ 37 , 38 , 39 , 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

Immunosensor with Enhanced Electrochemiluminescence Signal Using Platinum Nanoparticles Confined within Nanochannels for Highly Sensitive Detection of Carcinoembryonic Antigen

Zhang,

et al. 2023

Molecules

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Rapid, highly sensitive, and accurate detection of tumor biomarkers in serum is of great significance in cancer screening, early diagnosis, and postoperative monitoring. In this study, an electrochemiluminescence (ECL) immunosensing platform was constructed by enhancing the ECL signal through in situ growth of platinum nanoparticles (PtNPs) in a nanochannel array, which can achieve highly sensitive detection of the tumor marker carcinoembryonic antigen (CEA). An inexpensive and readily available indium tin oxide (ITO) glass electrode was used as the supporting electrode, and a layer of amino-functionalized vertically ordered mesoporous silica film (NH2-VMSF) was grown on its surface using an electrochemically assisted self-assembly method (EASA). The amino groups within the nanochannels served as anchoring sites for the one-step electrodeposition of PtNPs, taking advantage of the confinement effect of the ultrasmall nanochannels. After the amino groups on the outer surface of NH2-VMSF were derivatized with aldehyde groups, specific recognition antibodies were covalently immobilized followed by blocking nonspecific binding sites to create an immunorecognition interface. The PtNPs, acting as nanocatalysts, catalyzed the generation of reactive oxygen species (ROS) with hydrogen peroxide (H2O2), significantly enhancing the ECL signal of the luminol. The ECL signal exhibited high stability during continuous electrochemical scanning. When the CEA specifically bound to the immunorecognition interface, the resulting immune complexes restricted the diffusion of the ECL emitters and co-reactants towards the electrode, leading to a reduction in the ECL signal. Based on this immune recognition-induced signal-gating effect, the immunosensor enabled ECL detection of CEA with a linear range of 0.1 pg mL−1 to 1000 ng mL−1 with a low limit of detection (LOD, 0.03 pg mL−1). The constructed immunosensor demonstrated excellent selectivity and can achieve CEA detection in serum.

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Highly sensitive aptasensor for the detection of SARS-CoV-2-RBD using aptamer-gated methylene blue@mesoporous silica film/laser engraved graphene electrode

Cited by 16 publications

References 40 publications

Direct Detection of Viral Infections from Swab Samples by Probe‐Gated Silica Nanoparticle‐Based Lateral Flow Assay

Direct Detection of Viral Infections from Swab Samples by Probe‐Gated Silica Nanoparticle‐Based Lateral Flow Assay

Graphene-Based Electrochemical Nano-Biosensors for Detection of SARS-CoV-2

Immunosensor with Enhanced Electrochemiluminescence Signal Using Platinum Nanoparticles Confined within Nanochannels for Highly Sensitive Detection of Carcinoembryonic Antigen

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