A novel signal‐on fluorescent aptasensor for ochratoxin A detection based on RecJ<sub>f</sub> exonuclease‐induced signal amplification

Wei, Min; He, Xing; Xie, Yanli

doi:10.1002/jccs.201900423

Cited by 6 publications

(3 citation statements)

References 35 publications

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“…The three A bases at the 5′ end of Apt are favorable for RecJ f Exo to cleave the single strands in Apt–ZEN complex. [ 29,30 ]…”

Section: Methodsmentioning

confidence: 99%

A homogeneous electrochemical aptasensor based on DNA assembly for zearalenone detection

Xin

et al. 2021

J Chinese Chemical Soc

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In this work, a kind of homogeneous electrochemical aptasensor based on DNA assembly was designed to detect zearalenone (ZEN). The three DNA strands of ZEN aptamer (Apt), complementary strand DNA1, and complementary strand DNA2 are first mixed to form DNA duplex structure, which cannot be cleaved by RecJ f Exonuclease (RecJ f Exo) and hinders the electron transfer after addition to the electrode surface. With the existence of ZEN, the combination of ZEN and Apt destroys the duplex structure, and RecJ f Exo can catalyze the cleavage of Apt to form short DNA strands, which accelerates the electron transfer after addition to the electrode surface. According to the current difference with or without ZEN, the detection purpose is achieved. The linear relationship between I and LgC ZEN was found in the range of 5.0 Â 10 À6 ng/ml to 50 ng/ml, and the detection limit is as low as 0.51 fg/ml. The prepared sensor showed high selectivity, favorable repeatability, and reproducibility and was effectively used for testing the spiked corn flour and beer samples.

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“…The three A bases at the 5′ end of Apt are favorable for RecJ f Exo to cleave the single strands in Apt–ZEN complex. [ 29,30 ]…”

Section: Methodsmentioning

confidence: 99%

A homogeneous electrochemical aptasensor based on DNA assembly for zearalenone detection

Xin

et al. 2021

J Chinese Chemical Soc

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“…Ochratoxin A (OTA) is the most toxic and common ochratoxin and can lead to severe nephrotoxic, carcinogenic, teratogenic, mutagenic, genotoxic, and immunotoxic effects [2][3][4]. The International Agency for Research on Cancer has classified OTA as a Group 2B potential human carcinogen [5]. OTA is predominantly found in cereals, grapes, beer, wine, dried fruit, cocoa, coffee, and other foods [6,7].…”

Section: Introductionmentioning

confidence: 99%

Development of a Novel Magnetic-Bead-Based Automated Strategy for Efficient and Low-Cost Sample Preparation for Ochratoxin A Detection Using Mycotoxin–Albumin Interaction

Jin

Bao

Zheng

et al. 2023

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The mycotoxin ochratoxin A (OTA) is toxic to humans and frequently contaminates wine and beer. Antibodies are essential recognition probes for the detection of OTA. However, they have several drawbacks, such as high costs and difficulty in preparation. In this study, a novel magnetic-bead-based automated strategy for efficient and low-cost OTA sample preparation was developed. Human serum albumin, which is an economical and stable receptor based on the mycotoxin–albumin interaction, was adapted and validated to replace conventional antibodies to capture OTA in the sample. Ultra-performance liquid chromatography–fluorescence detection was used in combination with this preparation method for efficient detection. The effects of different conditions on this method were investigated. The recovery of OTA samples spiked at three different concentrations ranged from 91.2% to 102.1%, and the relative standard deviations (RSDs) were 1.2%–8.2% in wine and beer. For red wine and beer samples, the LODs were 0.37 and 0.15 µg/L, respectively. This reliable method overcomes the drawbacks of conventional methods and offers significant application prospects.

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“…However, this would greatly reduce sensitivity. To solve the problem of low sensitivity, enzyme amplification techniques were usually introduced into FABs. ,, For example, Wu et al introduced a ligation-rolling circle amplification (L-RCA) technique into the aptamer beacon of platelet-derived growth factor (PDGF). Upon target binding, the aptamer structure switching would trigger the L-RCA process, which resulted in a detection limit (LOD) of PDGF at the attomolar scale …”

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confidence: 99%

Versatile Integration of Liquid-Phase Microextraction and Fluorescent Aptamer Beacons: A Synergistic Effect for Bioanalysis

et al. 2021

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Fluorescent aptamer beacons (FABs) are a major category of biosensors widely used in environmental analysis. However, due to their low compatibility, it is difficult to use the common FABs for biological samples. To overcome this challenge, construction of FABs with complex structures to adapt the nature of biological samples is currently in progress in this field. Unlike previous works, we moved our range of vision from the FAB itself to the biological sample. Inspired by this idea, in this work, flat membrane-based liquid-phase microextraction (FM-LPME) with sufficient sample cleanup and preconcentration capacities was integrated with FABs. With the merits of both FM-LPME and FABs, the integrated LPME-FAB system displayed a clear synergistic enhancement for target analysis. Specifically, LPME in the LPME-FAB system provided purified and enriched Hg 2+ for the FAB recognition, while the FAB recognition event promoted the extraction efficiency of LPME. Due to superior performances, the LPME-FAB system achieved highly sensitive analysis of Hg 2+ in urine samples with a detection limit of 27 nM and accuracies in the range of 98−113%. To the best of our knowledge, this is the first time that an integrated LPME-FAB system was constructed for target analysis in biological samples. We believe that this study will provide a new insight into the next generation of biosensors, where the integration of sample preparation with detection probes is as important as the design of complex probes in the field of bioanalysis.

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A novel signal‐on fluorescent aptasensor for ochratoxin A detection based on RecJ_f exonuclease‐induced signal amplification

Cited by 6 publications

References 35 publications

A homogeneous electrochemical aptasensor based on DNA assembly for zearalenone detection

A homogeneous electrochemical aptasensor based on DNA assembly for zearalenone detection

Development of a Novel Magnetic-Bead-Based Automated Strategy for Efficient and Low-Cost Sample Preparation for Ochratoxin A Detection Using Mycotoxin–Albumin Interaction

Versatile Integration of Liquid-Phase Microextraction and Fluorescent Aptamer Beacons: A Synergistic Effect for Bioanalysis

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A novel signal‐on fluorescent aptasensor for ochratoxin A detection based on RecJf exonuclease‐induced signal amplification

Cited by 6 publications

References 35 publications

A homogeneous electrochemical aptasensor based on DNA assembly for zearalenone detection

A homogeneous electrochemical aptasensor based on DNA assembly for zearalenone detection

Development of a Novel Magnetic-Bead-Based Automated Strategy for Efficient and Low-Cost Sample Preparation for Ochratoxin A Detection Using Mycotoxin–Albumin Interaction

Versatile Integration of Liquid-Phase Microextraction and Fluorescent Aptamer Beacons: A Synergistic Effect for Bioanalysis

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A novel signal‐on fluorescent aptasensor for ochratoxin A detection based on RecJ_f exonuclease‐induced signal amplification