A homogeneous electrochemical aptasensor based on DNA assembly for zearalenone detection

Abstract

In this work, a kind of homogeneous electrochemical aptasensor based on DNA assembly was designed to detect zearalenone (ZEN). The three DNA strands of ZEN aptamer (Apt), complementary strand DNA1, and complementary strand DNA2 are first mixed to form DNA duplex structure, which cannot be cleaved by RecJ f Exonuclease (RecJ f Exo) and hinders the electron transfer after addition to the electrode surface. With the existence of ZEN, the combination of ZEN and Apt destroys the duplex structure, and RecJ f Exo can catalyze the cleavage of Apt to form short DNA strands, which accelerates the electron transfer after addition to the electrode surface. According to the current difference with or without ZEN, the detection purpose is achieved. The linear relationship between I and LgC ZEN was found in the range of 5.0 Â 10 À6 ng/ml to 50 ng/ml, and the detection limit is as low as 0.51 fg/ml. The prepared sensor showed high selectivity, favorable repeatability, and reproducibility and was effectively used for testing the spiked corn flour and beer samples.

Detection of AFB1 in corn by MXene paper‐based unlabeled aptasensor

“…The Chinese national standards specifics a maximum limit of less than 20 μg/kg in food in corn, corn flakes, and corn products. The common laboratory methods for detecting AFB1 are immunochromatography (Liu et al, 2020), chemiluminescence (Shim et al, 2014), high‐performance liquid chromatography (Zhang et al, 2020), chromatography–mass spectrometry (Dong et al, 2018), and so on (Qu et al, 2021). However, these approaches are time‐consuming and expensive and require sample preparation and trained personnel to operate the instruments.…”

“…, chromatography-mass spectrometry (Dong et al, 2018), and so on (Qu et al, 2021). However, these approaches are timeconsuming and expensive and require sample preparation and trained personnel to operate the instruments.…”

An immobilization-free electrochemical aptamer-based assay for zearalenone based on target-triggered dissociation of DNA from polydopamine nanospheres with strand displacement amplification

“…Since the aptamer for ZEN was screened in 2012, 8 it has been applied to the development of various aptasensors for ZEN. [9][10][11][12][13][14][15][16] In order to achieve efficient signal amplication, nucleic acid amplication strategies are powerful tools. 17 PCR is a classic nucleic acid amplication process, which requires complex thermal cycling.…”

Evanescent Wave Optical-Fiber Aptasensor for Rapid Detection of Zearalenone in Corn with Unprecedented Sensitivity

“…Refs. [ 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ] are cite in Supplementary Materials file.…”