Development of a Novel Magnetic-Bead-Based Automated Strategy for Efficient and Low-Cost Sample Preparation for Ochratoxin A Detection Using Mycotoxin–Albumin Interaction

Abstract

The mycotoxin ochratoxin A (OTA) is toxic to humans and frequently contaminates wine and beer. Antibodies are essential recognition probes for the detection of OTA. However, they have several drawbacks, such as high costs and difficulty in preparation. In this study, a novel magnetic-bead-based automated strategy for efficient and low-cost OTA sample preparation was developed. Human serum albumin, which is an economical and stable receptor based on the mycotoxin–albumin interaction, was adapted and validated to replace conventional antibodies to capture OTA in the sample. Ultra-performance liquid chromatography–fluorescence detection was used in combination with this preparation method for efficient detection. The effects of different conditions on this method were investigated. The recovery of OTA samples spiked at three different concentrations ranged from 91.2% to 102.1%, and the relative standard deviations (RSDs) were 1.2%–8.2% in wine and beer. For red wine and beer samples, the LODs were 0.37 and 0.15 µg/L, respectively. This reliable method overcomes the drawbacks of conventional methods and offers significant application prospects.

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

“…Third, the multiple off‐line steps of “pre‐sampling (extraction, filtration, and purification)” prior to “analysis” hamper the reliable detection of mycotoxins in foodstuffs, since the errors resulting from manual processing are inevitable. Hence, the development of automated and portable devices that integrate magnetic immunoaffinity‐based clean‐up and detection steps, such as CHRONECT Symbiosis RIDA ® CREST, would greatly shorten the processing duration, expand their applications for multiple samples, and enhance their anti‐interference capacity against complex foodstuffs (Ni, Ye et al., 2022; Ye et al., 2023). Fourth, the confirmation of the MNP‐based biosensors for commodities testing has not yet been validated.…”