A Novel P106L Mutation in EPSPS and an Unknown Mechanism(s) Act Additively To Confer Resistance to Glyphosate in a South African <i>Lolium rigidum</i> Population

Kaundun, Shiv Shankhar; Dale, Richard P.; Zelaya, Ian A.; Dinelli, Giovanni; Marotti, Ilaria; McIndoe, Eddie; Cairns, A. L. P.

doi:10.1021/jf104934j

Cited by 81 publications

(84 citation statements)

References 43 publications

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“…Plants that have mutations in the amino acid sequence in EPSPs confer low levels of herbicide resistance, about two to four times (Kaundun et al, 2011), while the resistance to action mechanisms of ACCase, ALS, and photosystem II inhibitors provide levels between 20 and 100 times, compared to the susceptible (Devine & Shukla, 2000).…”

Section: Resultsmentioning

confidence: 99%

Glyphosate influence on the physiological parameters of Conyza bonariensis biotypes

et al. 2014

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-The objective of this study was to determine changes in gas exchange and inhibition of EPSPs, based on the accumulation of shikimic acid in horseweed biotypes resistant and sensitive to glyphosate. Two experiments were conducted in a factorial model. The first one evaluated horseweed biotypes (one resistant and one susceptible to glyphosate), and herbicide rates (0 and 1,080 g a.e. ha 1 ) applied on the weed. In the second experiment, the horseweed biotypes (susceptible and resistant to glyphosate) were evaluated in five periods as following: 0, 3, 7, 10, and 14 days after herbicide application (DAH). The photosynthetic rate, transpiration, carboxylation efficiency, and water efficiency were determined using an infrared gas analyzer (IRGA), and shikimic acid concentration by HPLC. The application of glyphosate damaged the photosynthetic parameters of the susceptible biotype, causing complete inhibition of the photosynthetic rate, transpiration rate, carboxylation efficiency and water use efficiency, starting from the 7 DAH. On the other hand, total inhibition of the photosynthetic parameters was not observed for the resistant biotype. Shikimic acid accumulation occurred in both biotypes after glyphosate application but the susceptible biotype had the highest concentrations, indicating greater sensitivity of the enzyme EPSPs. The accumulation of shikimic acid in the resistant biotype indicates that the mechanism of resistance is not related to the total insensitivity of the enzyme EPSPs to glyphosate and/or that other resistance mechanisms may be involved.Keywords: hairy fleabane, resistance, photosynthesis, EPSPs, shikimic acid. RESUMO -Objetivou-se neste trabalho determinar alterações na fotossíntese e na inibição da EPSPs

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Section: Resultsmentioning

confidence: 99%

Glyphosate influence on the physiological parameters of Conyza bonariensis biotypes

et al. 2014

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“…The resistance mechanism to glyphosate in other countries' goosegrass involves replacement of a proline by serine or threonine at position 106 in the EPSPS gene (P106S or P106T) (Baerson et al, 2002;Ng et al2004;Yu et al2007;Kaundun et al, 2008Kaundun et al, , 2011, or the EPSPS gene amplification in resistant plants (Chen et al, 2015). Such single mutation confers intermediate levels of glyphosate resistance (RF = 2 to 4).…”

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confidence: 99%

Goosegrass Resistant to Glyphosate in Brazil

et al. 2017

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-Several cases of herbicide resistance in goosegrass have been confirmed worldwide. Reports of control failures after glyphosate application have been observed, especially in the Midwest region of Paraná State. The objective of this study was to evaluate the existence of goosegrass populations resistant to glyphosate. For this, 25 populations collected in two consecutive seasons (2013/ 2014 and 2014/2015) were sown and grown in greenhouse. Glyphosate dose-response curve experiments were performed using doses of 0, 60, 120, 240, 480, 960, 1,920, 3,840, 7,680 and 15,360 g a.e. ha -1 . The application stages were from two to three tillers (E1) for the populations of 2013/2014 and E1 and five to six tillers (E2) for the populations of 2014/2015. Furthermore, three of the populations supposedly considered resistant in these experiments (populations 7, 19 and 25) have had their F1 submitted to the herbicide dose-response test in order to verify whether the resistance was inheritable. With the results obtained in this study, it was concluded that the populations 19 and 25, from Campo Mourão and Luziânia (Midwest of Paraná) are the first confirmed cases of goosegrass resistant to glyphosate in Brazil (RF = 3.99 to 6.81), following all the criteria for confirmation of new weed resistance cases. (2013/2014 e 2014/2015) foram semeadas e cultivadas em casa de vegetação. Experimentos de curva de dose-resposta de glyphosate foram realizados utilizando-se as doses de 0, 60, 120, 240, 480, 960, 1.920, 3.840, 7.680 e 15.360 g e.a. ha -1 Keywords: Eleusine indica, dose-response, application timming, resistance factor. RESUMO -Diversos casos de resistência de capim-pé-de-galinha têm

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“…The EPSPs partial sequence for resistant and susceptible ryegrass plants analyzed did not show any mutation at Pro 106 ( Table 2) that has been associated with glyphosate resistance reported for the Lolium rigidum Gaudin (Kaundun et al, 2011) and Lolium multiflorum (Perez-Jones et al, 2005) species. However, the results found corroborate with those by Salas et al (2012), which did not observe any mutation on the EPSPs gene on Lolium perenne biotypes.…”

Section: Epsps Gene Sequencingmentioning

confidence: 87%

“…When related to the site of action, resistance may be a result of the reduced affinity of the herbicide for the site of action (enzyme), as a result of DNA mutation, or due to the overexpression of this enzyme. Different EPSPs gene mutations have been reported for conferring resistance to glyphosate on Lolium rigidum (Kaundun et al, 2011), Lolium multiflorum (Perez-Jones et al, 2005) and Echinochloa colona (Alarcón-Reverte et al, 2013). When the resistance is not related to the site of action, it may occur due to mechanisms such as reduced absorption, reduced or increased translocation and increased metabolism and compartmentalization (Powles and Yu, 2010).…”

Section: Introductionmentioning

confidence: 99%

Verification of the Mechanism of Glyphosate Resistance in Italian Ryegrass Biotypes

et al. 2016

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-The intense use of glyphosate for weed control led to the emergence of several cases of resistance to this herbicide. Weeds can survive the application of herbicides due to several factors, which may or may not be related to the herbicide site of action. The objectives of this study were to quantify the accumulation of shikimate in ryegrass biotypes in response to glyphosate application; investigate possible mutations on the EPSPs gene in susceptible and resistant biotypes; and evaluate the response of ryegrass biotypes to the application of glyphosate after treatment with a metabolism inhibitor of cyt P450 monooxygenase. The seeds of ryegrass biotypes with suspected resistance came from the municipality of São Valentim, RS (SVA 1 and SVA 4) and Passo Fundo, RS (PFU 5) and the seeds of the susceptible biotype (SVA 2), from São Valentim. The results demonstrated that, SVA biotype 2 accumulated more shikimate than any of the resistant biotypes, regardless of the herbicide dose used. The EPSPs gene showed no point mutation previously associated with the resistance to glyphosate, and the evaluated biotypes show no metabolism of glyphosate by the cyt P450 complex concerning inhibition by piperonyl butoxide (PBO) and malathion.Keywords: shikimic acid, EPSPs, Lolium multiflorum, metabolism. RESUMO -A intensa utilização do glyphosate para controle de plantas daninhas levou ao surgimento de vários casos de resistência a esse herbicida. As plantas daninhas podem sobreviver à aplicação de herbicidas devido a diversos fatores, os quais podem estar relacionados ou não

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A Novel P106L Mutation in EPSPS and an Unknown Mechanism(s) Act Additively To Confer Resistance to Glyphosate in a South African Lolium rigidum Population

Cited by 81 publications

References 43 publications

Glyphosate influence on the physiological parameters of Conyza bonariensis biotypes

Glyphosate influence on the physiological parameters of Conyza bonariensis biotypes

Goosegrass Resistant to Glyphosate in Brazil

Verification of the Mechanism of Glyphosate Resistance in Italian Ryegrass Biotypes

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