Verification of the Mechanism of Glyphosate Resistance in Italian Ryegrass Biotypes

Várgas, Leandro; Ruchel, Q.; Agostinetto, Dirceu; Lamego, Fabiane Pinto; Langaro, Ana Cláudia; Piesanti, Sandro Roberto

doi:10.1590/s0100-83582016340300017

Cited by 4 publications

(4 citation statements)

References 16 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…So, the aims of this work are firstly to test the in vitro phytotoxic activity (of previously analysed commercial essential oils, winter savory, peppermint, and anise, in order to assure their main compounds by GC/MS) against seed germination and seedling growth of P. oleracea , a cosmopolitan annual weed of tropical and subtropical climates; L. multiflorum , because Lolium spp. has been ranked as one of the specimens most frequently exhibiting herbicide resistance in many countries [30,31,32]; and E. crus-galli , a serious weed of irrigation crops, especially rice. Secondly, we aim to corroborate in vivo the previous in vitro phytotoxic effect using a commercial emulsifiable concentrate with the more phytotoxic essential oils and finally, we will study their potential hazard against maize ( Zea mays L.), rice ( Oryza sativa L.), and tomato ( Solanum lycopersicum L.) seeds in order to obtain selective bioherbicides for food crops.…”

Section: Introductionmentioning

confidence: 99%

Phytotoxicity of Essential Oils on Selected Weeds: Potential Hazard on Food Crops

Ibáñez

Blázquez

2018

Plants

View full text Add to dashboard Cite

The chemical composition of winter savory, peppermint, and anise essential oils, and in vitro and in vivo phytotoxic activity against weeds (Portulaca oleracea, Lolium multiflorum, and Echinochloa crus-galli) and food crops (maize, rice, and tomato), have been studied. Sixty-four compounds accounting for between 97.67–99.66% of the total essential oils were identified by Gas Chromatography-Mass Spectrometry analysis. Winter savory with carvacrol (43.34%) and thymol (23.20%) as the main compounds produced a total inhibitory effect against the seed germination of tested weed. Menthol (48.23%), menthone (23.33%), and iso-menthone (16.33%) from peppermint only showed total seed germination inhibition on L. multiflorum, whereas no significant effects were observed with trans-anethole (99.46%) from anise at all concentrations (0.125–1 µL/mL). Low doses of peppermint essential oil could be used as a sustainable alternative to synthetic agrochemicals to control L. multiflorum. The results corroborate that in vivo assays with a commercial emulsifiable concentrate need higher doses of the essential oils to reproduce previous in vitro trials. The higher in vivo phytotoxicity of winter savory essential oil constitutes an eco-friendly and less pernicious alternative to weed control. It is possible to achieve a greater in vivo phytotoxicity if less active essential oil like peppermint is included with other active excipients.

show abstract

Section: Introductionmentioning

confidence: 99%

Phytotoxicity of Essential Oils on Selected Weeds: Potential Hazard on Food Crops

Ibáñez

Blázquez

2018

Plants

View full text Add to dashboard Cite

show abstract

“…A total of 25 seedlings from five glyphosate-resistant diploid Italian ryegrass plants (SVA04 biotype) harvested in São Valentin-RS, previously characterized with nontarget site resistance (Vargas et al 2016), were transplanted separately in trays containing soil and substrate in a 3:1 proportion. Glyphosate herbicide (Glifosato Atanor 48) at a rate of 2160 g a.e.•ha -1 was applied at the beginning tillering stage to confirm the herbicide-resistance.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Italian ryegrass (Lolium multiflorum Lam.) is a troublesome winter annual grass found in agricultural systems cultivated with wheat and barley, and widely used as forage to animal feed and soil cover plant (Vargas et al 2016). In the last years, the area cultivated with tetraploid Italian ryegrass has been increasing in the Southern Brazilian fields due to the occurrence of diploid glyphosate-resistant populations, and its characteristics such as higher yield of pasture and nutritive value compared to diploid plants (Oliveira et al 2014).…”

Section: Introductionmentioning

confidence: 99%

May the crossing between diploid and tetraploid Italian ryegrass transfer glyphosate resistance to the next generation?

et al. 2020

View full text Add to dashboard Cite

Italian ryegrass (Lolium multiflorum Lam.) is a grass weed found in winter cereals, with several glyphosate-resistant diploid populations. Furthermore, the use of Italian ryegrass tetraploid genotypes creates an opportunity to increase the forage demand in replacement of diploid populations. The aim of this research was to evaluate the crossing capacity between glyphosate-resistant diploid and tetraploid Italian ryegrass to transfer glyphosate resistance to the next generation. Reciprocal crosses were performed using the susceptible tetraploid cultivar INIA Titan and resistant diploid (SVA04 biotype) plants to obtain the F1 progeny. Germination testing, survival percentage, inheritance of glyphosate-resistance, and ploidy level were evaluated in F1 progeny. Crossing of susceptible tetraploid plants ♂ and glyphosateresistant diploid ♀ affected the germination and exhibited 4.5% of triploid plants. F1 progeny from the glyphosate-resistant diploid ♂ and susceptible tetraploid ♀ did not show germination problems, but 46% of the plants died during the initial seedling stage. For this progeny, 10 and 25% of the seedlings were composed of triploid and pentaploid individuals, respectively. Crosses between susceptible tetraploid ♀ and resistant diploid ♂ did not transfer the glyphosateresistant for the plants. Susceptible tetraploid ♂ crossing with diploid resistant ♀ had a reduction of 47% the glyphosate-resistant plants compared to the homozygous glyphosateresistant progeny. Overall, tetraploid Italian ryegrass can be a safe tool to reduce and manage the increase of glyphosate-resistant diploid ryegrass in field conditions.

show abstract

“…The resistance mechanism of ryegrass to glyphosate has not yet been fully understood (Vargas et al, 2016). Some studies have suggested a reduction in the absorption and translocation (Ghanizadeh et al, 2016;Fernández-Moreno et al, 2017) and others the overexpression of the EPSPs enzyme (Salas et al, 2015) and the sequestration of the herbicide in the vacuole (Ge et al, 2012).…”

Section: Figure 6 -mentioning

confidence: 99%

Quick Test for Detecting Glyphosate-Resistant Ryegrass Seeds

et al. 2019

Self Cite

View full text Add to dashboard Cite

The commercialization of ryegrass seeds with the presence of resistant biotypes is a serious issue that increses the dispersion of resistance to new areas without this problem. Therefore, this study aimed to develop a quick test to detect susceptible and resistant seeds to the glyphosate herbicide in order to identify contaminated seed lots with glyphosate resistance. Three experiments were carried out, one in greenhouse and the other two in the seed laboratory. First, the resistance factor (RF) was determined by a dose-response curve experiment in biotypes suspected to be resistant and susceptible to glyphosate. Then, the germination test was conducted with the selected biotypes under increasing glyphosate rates (0, 3.5, 6.9, 13.9, 27.8, 55.5, 111, 222, 445, 890, and 1,780 mg a.e. L -1 ), as the second experiment. The third experiment was made to verify the methodological efficiency of the germination test with glyphosate to identify different contamination ratios (0, 4, 12, 36, and 100%) of the resistant biotype in the seed lot. The different levels of susceptibility of the biotypes to glyphosate were confirmed by the RF of 154.7 based on C 50 . Germination of the susceptible biotype was inhibited more than 99% by the rate of 127 mg a.e. L -1 , while the resistant biotype was a little affected. The germination test with 127 mg a.e. L -1 of glyphosate showed contaminations of 2, 5, 19, 39, and 86% in lots with 0, 4, 12, 36, and 100% of contaminated seeds, respectively. This methodology can detect glyphosate susceptible and resistant seeds and identify contaminated seed lots with resistant glyphosate biotypes.Planta Daninha 2019; v37:e019187165 SCHMITZ, M.F. et al. Quick test for detecting glyphosate-resistant ryegrass seeds 2 em lotes com a presença de 0, 4, 12, 36 e 100% de sementes contaminadas, respectivamente, quando expostas à dose de 127 mg e.a. L -1 . A metodologia testada possibilitou diferenciar sementes suscetíveis e resistentes ao herbicida glifosato e identificar lotes de sementes contaminadas com biótipos resistentes.Palavras-chave: Lolium multiflorum, germinação, herbicida, metodologia.

show abstract

Verification of the Mechanism of Glyphosate Resistance in Italian Ryegrass Biotypes

Cited by 4 publications

References 16 publications

Phytotoxicity of Essential Oils on Selected Weeds: Potential Hazard on Food Crops

Phytotoxicity of Essential Oils on Selected Weeds: Potential Hazard on Food Crops

May the crossing between diploid and tetraploid Italian ryegrass transfer glyphosate resistance to the next generation?

Quick Test for Detecting Glyphosate-Resistant Ryegrass Seeds

Contact Info

Product

Resources

About