A novel impedimetric disposable immunosensor for rapid detection of a potential cancer biomarker

Asav, Engin; Sezgintürk, Mustafa Kemal

doi:10.1016/j.ijbiomac.2014.02.032

Cited by 43 publications

(20 citation statements)

References 33 publications

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“…Likewise, the imaginary portion of the experimental data was used to calculate the real part of a stable, linear, and causal circuit. By this calculation, "Goodness of Fit" values were also procured (Asav and Sezgintürk, 2014;Şimşek et al, 2015). Goodness of fit values can be seen in Supplemental information Table 1.…”

Section: Analytical Characteristics Of the Biosensormentioning

confidence: 99%

AuNPs modified, disposable, ITO based biosensor: Early diagnosis of heat shock protein 70

Karaboğa

Şimşek

Sezgintürk

2016

Biosensors and Bioelectronics

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Section: Analytical Characteristics Of the Biosensormentioning

confidence: 99%

AuNPs modified, disposable, ITO based biosensor: Early diagnosis of heat shock protein 70

Karaboğa

Şimşek

Sezgintürk

2016

Biosensors and Bioelectronics

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“…Lately, nanomaterials based electrochemical immunosensors have become popular since they offer enhanced biomolecule loading capacity to increase the dynamic range of measurements. The technique has been extended by employing various nano-architectured electrode surfaces for determination of several carcinoma biomarkers-CA125 [14,15], human epidermal growth factor receptor [16], human chorionic gonadotropin [17] to biomolecules-interleukin-6 [18] and inorganic molecules-dopamine [19] and okadaic acid [20]. The reports for impedimetric immunosensor for CA125 are limited.…”

Section: Introductionmentioning

confidence: 99%

Core–shell gold–silver nanoparticles based impedimetric immunosensor for cancer antigen CA125

Raghav

Srivastava

2015

Sensors and Actuators B: Chemical

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“…When the I-V behavior of TRIS-PAGE-PGEs and PAGE-PGEs is compared with the PGEs, a reduction in anodic and cathodic current (Figure 3) is demonstrated due to the reduced electron transfer rate of the redoks pair as a result of the polymer coating [16]. The decrease in oxidative and reductive peak current may be due to the changes in load transfer resistance of electrode surface [19,20].…”

Section: Discussionmentioning

confidence: 99%

“…For the electrochemical analysis, 20 mM K 3 [Fe(CN) 6 ] redox pair in phosphate buffer solution was used (redoks-PBS, 137mM NaCl, 2.7mM KCl, 10mM Na 2 HPO 4 , 1.8mM KH 2 PO 4 , pH 7.4) [14][15][16].…”

Section: Materials and Experimental Methodsmentioning

confidence: 99%

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A Molecularly Imprinted Polymer Based Biosensor for Electrochemical Impedance Spectroscopic Analysis

Utku¹,

Ozdemir²,

Bakay³

2018

IU-JEEE

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Biosensor, a device used in the detection of an analyte, combines a biological/chemical sensor component with a physicochemical transducer. The sensor and the transducer elements recognize and detect the analyte qualitatively and/or quantitatively [1][2][3]. The biological sensor element may be in the form of tissue, microorganism, organelle, receptor, enzyme, antibody, nucleic acid, molecule, etc., which may be attached to the metal, polymer or glass surface of the electrode through chemical and physical means.A biological sensor element may be relatively short-lived and with complications in handling; therefore, they may be replaced with artificial elements that are components of a receptor-based sensing system. Molecular Imprinting Technology (MIT) is a method that aims to overcome these complications by producing selectively specific artificial receptors. It utilizes molecular imprinting polymer (MIP), formed as a dependable molecular recognition element with room temperature stability that mimicks natural recognition elements, such as antibodies and receptors. MIT is used in the detection, separation and purification of biological and chemical molecules, such as amino acids and proteins, nucleotids, toxins, drugs, etc. A 3D polymeric network is formed between the analyte and monomer through functional hydrogen bonds, dipole-dipole and ionic interactions. After polymerization, upon removal of the analyte, specific recognition sites that are in the shape, size and chemical structure of the analyte are formed in the polymer [4][5][6][7][8][9][10][11].There are examples of MIP based sensors equipped more commonly with SPRS and QCM based transducers [4]. However, they have not been experimented in the detection of small ABSTRACTA molecularly imprinted polymer (MIP)-based impedimetric biosensor was developed for the electrochemical analysis of low-weight biological molecules. Synthetic polymeric matrices with specific and selective recognition sites, which are complementary to the shapes and sizes of the functional groups of analytes, can be prepared using the molecular imprinting method. In this study, a small molecule, tris(hydroxymethyl)aminomethane (TRIS), was used to coat a graphite pencil tip with a TRIS-containing polyacrylamide gel to fabricate a working electrode. The electrode modification and performance were evaluated using cyclic voltammetry and electrochemical impedance spectroscopy. The electrochemical properties of the modified electrodes were observed using an electrochemical cell comprising a Ag/AgCl reference electrode, a Pt wire as the counter electrode, and a pencil graphite tip as the working electrode using a redox-phosphate buffer solution with different concentrations of TRIS and Ethylenediaminetetraacetic acid (EDTA). The I-V and impedance performance of the chemically modified graphite pencil-tip electrodes exhibited decreased conductance and increased impedance correlating with the increase in TRIS concentration. Thus, MIP-based small-molecule biosensor prototypes can be promising eco...

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A novel impedimetric disposable immunosensor for rapid detection of a potential cancer biomarker

Cited by 43 publications

References 33 publications

AuNPs modified, disposable, ITO based biosensor: Early diagnosis of heat shock protein 70

AuNPs modified, disposable, ITO based biosensor: Early diagnosis of heat shock protein 70

Core–shell gold–silver nanoparticles based impedimetric immunosensor for cancer antigen CA125

A Molecularly Imprinted Polymer Based Biosensor for Electrochemical Impedance Spectroscopic Analysis

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