Core–shell gold–silver nanoparticles based impedimetric immunosensor for cancer antigen CA125

Raghav, Ragini; Srivastava, Sudha

doi:10.1016/j.snb.2015.05.108

Cited by 59 publications

(24 citation statements)

References 34 publications

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“…The photostable fluorescence emission arising in a number of nanomaterial classes due to quantum confinement and edge effects may allow one to monitor the activity of the drug and its release into the tissues in real time [ 8 ]. A variety of different nanoparticles have shown promising potential in anticancer drug delivery and imaging, including inorganic quantum dots [ 9 ], silicon nanomaterials [ 10 ], silver nanoparticles [ 11 ], liposomes [ 12 ], and gold nanoparticles [ 13 ]. Gold nanoparticles include nanoshells [ 14 ], nanorods [ 15 ], and other nanoscale gold constructs utilized for drug delivery [ 16 ], early cancer cell identification by EGFR (epidermal growth factor receptor) overexpression [ 17 ], or Raman-based detection of epithelial cancers [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…Simple models have already been used to make predictions about the effectiveness of cancer treatments [ 41 , 42 ], including combination therapies [ 39 , 43 ]. Current strategies for modeling efficacies of drugs alone use the IC 50 (concentration of the drug at half maximum effect) measurement and, less commonly, the E max (the maximum reduction in the growth rate [ 11 ]) measurement to give the correct drug dosage to a patient. The real time-dependent nature of these curves [ 44 ] can lead to inaccuracies in theoretical models due to their bias toward exponential growth and delays in drug effect stabilization, [ 44 , 45 ], thus requiring new modeling approaches that can estimate time-independent parameters.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Doxorubicin Delivery by Nitrogen-Doped Graphene Quantum Dots on Cancer Cell Growth: Experimental Study and Mathematical Modeling

et al. 2021

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With 18 million new cases diagnosed each year worldwide, cancer strongly impacts both science and society. Current models of cancer cell growth and therapeutic efficacy in vitro are time-dependent and often do not consider the Emax value (the maximum reduction in the growth rate), leading to inconsistencies in the obtained IC50 (concentration of the drug at half maximum effect). In this work, we introduce a new dual experimental/modeling approach to model HeLa and MCF-7 cancer cell growth and assess the efficacy of doxorubicin chemotherapeutics, whether alone or delivered by novel nitrogen-doped graphene quantum dots (N-GQDs). These biocompatible/biodegradable nanoparticles were used for the first time in this work for the delivery and fluorescence tracking of doxorubicin, ultimately decreasing its IC50 by over 1.5 and allowing for the use of up to 10 times lower doses of the drug to achieve the same therapeutic effect. Based on the experimental in vitro studies with nanomaterial-delivered chemotherapy, we also developed a method of cancer cell growth modeling that (1) includes an Emax value, which is often not characterized, and (2), most importantly, is measurement time-independent. This will allow for the more consistent assessment of the efficiency of anti-cancer drugs and nanomaterial-delivered formulations, as well as efficacy improvements of nanomaterial delivery.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Doxorubicin Delivery by Nitrogen-Doped Graphene Quantum Dots on Cancer Cell Growth: Experimental Study and Mathematical Modeling

et al. 2021

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“…Both substrates are characterized by a similar value of impedance module, but the AgNPs/TNT platform (25 cycles) contains approximately 56% of silver weight less than Ag/TNT, which indicates that the elaborated electrode has a large surface area. Achieved results, according to results described in literature [48,49,50,51,52,53,54,55] in which the addition of silver nanoparticles is deposited onto various substrates, caused a decrease in the impedance module of the created sensor, in some cases even more than by 50%. Structures formed after 10, 15, and 30 cycles of deposition were characterized by lower conductivity, possibly due to the presence of agglomerates, which impede the electron transfer along a tubular structure.…”

Section: Resultsmentioning

confidence: 70%

Influence of the Silver Nanoparticles (AgNPs) Formation Conditions onto Titanium Dioxide (TiO2) Nanotubes Based Electrodes on Their Impedimetric Response

2019

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This paper presents the comparison of the effects of three methods of production of silver spherical and near-spherical nanoparticles (AgNPs) on the titanium dioxide nanotubes (TNT) base: cyclic voltammetry, chronoamperometry, and sputter deposition. It also evaluates the influence of silver nanoparticles on the electrochemical properties of the developed electrodes. The novelty of this research was to fabricate regular AgNPs free of agglomerates uniformly distributed onto the TNT layer, which has not been accomplished with previous attempts. The applied methods do not require stabilizing and reducing reagents. The extensive electrochemical characteristic of AgNP/TNT was performed by open circuit potential and electrochemical impedance spectroscopy methods. For AgNPs/TNT obtained by each method, the impedance module of these electrodes was up to 50% lower when compared to TNT, which means that AgNPs enabled more efficient electron transfer due to the effective area increase. In addition, the presence of nanoparticles increases the corrosion resistance of the prepared electrodes. These substrates can be used as electrochemical sensors due to their high electrical conductivity, and also as implants due to the antibacterial properties of both the TNT and AgNPs.

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“…The carboxylic encapsulated gold nanoparticles having 5 nm size were incubated on the surface of the SAM modified sensor for 12 h. After the incubation step, the gold nanoparticles surface was activated using 50 mM of EDC and NHS. This process enables the antibodies to attach covalently to the carboxylic gold nanoparticles [27]. The surface activation of the carboxylic gold nanoparticles is shown in Fig.…”

Section: Methods/experimentalmentioning

confidence: 99%

Detection of cancer antigens (CA-125) using gold nano particles on interdigitated electrode-based microfluidic biosensor

et al. 2019

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Integrating microfluidics with biosensors is of great research interest with the increasing trend of lab-on-the chip and point-of-care devices. Though there have been numerous studies performed relating microfluidics to the biosensing mechanisms, the study of the sensitivity variation due to microfluidic flow is very much limited. In this paper, the sensitivity of interdigitated electrodes was evaluated at the static drop condition and the microfluidic flow condition. In addition, this study demonstrates the use of gold nanoparticles to enhance the sensor signal response and provides experimental results of the capacitance difference during cancer antigen-125 (CA-125) antigen–antibody conjugation at multiple concentrations of CA-125 antigens. The experimental results also provide evidence of disease-specific detection of CA-125 antigen at multiple concentrations with the increase in capacitive signal response proportional to the concentration of the CA-125 antigens. The capacitive signal response of antigen–antibody conjugation on interdigitate electrodes has been enhanced by approximately 2.8 times (from 260.80 to 736.33 pF at 20 kHz frequency) in static drop condition and approximately 2.5 times (from 205.85 to 518.48 pF at 20 kHz frequency) in microfluidic flow condition with gold nanoparticle-coating. The capacitive signal response is observed to decrease at microfluidic flow condition at both plain interdigitated electrodes (from 260.80 to 205.85 pF at 20 kHz frequency) and gold nano particle coated interdigitated electrodes (from 736.33 to 518.48 pF at 20 kHz frequency), due to the strong shear effect compared to static drop condition. However, the microfluidic channel in the biosensor has the potential to increase the signal to noise ratio due to plasma separation from the whole blood and lead to the increase concentration of the biomarkers in the blood volume for sensing.

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Core–shell gold–silver nanoparticles based impedimetric immunosensor for cancer antigen CA125

Cited by 59 publications

References 34 publications

Effects of Doxorubicin Delivery by Nitrogen-Doped Graphene Quantum Dots on Cancer Cell Growth: Experimental Study and Mathematical Modeling

Effects of Doxorubicin Delivery by Nitrogen-Doped Graphene Quantum Dots on Cancer Cell Growth: Experimental Study and Mathematical Modeling

Influence of the Silver Nanoparticles (AgNPs) Formation Conditions onto Titanium Dioxide (TiO2) Nanotubes Based Electrodes on Their Impedimetric Response

Detection of cancer antigens (CA-125) using gold nano particles on interdigitated electrode-based microfluidic biosensor

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