AuNPs modified, disposable, ITO based biosensor: Early diagnosis of heat shock protein 70

Karaboğa, Münteha Nur Sonuç; Şimşek, Çiğdem Sayıklı; Sezgintürk, Mustafa Kemal

doi:10.1016/j.bios.2015.08.044

Cited by 60 publications

(14 citation statements)

References 35 publications

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“…Cyclic voltammograms of AuNBPs/SAM/ITO (curve i) electrodes exhibited well-defined oxidation and reduction potential peaks at 0.24 V and 0.04 V, respectively, whereas anti-AFB 1 /AuNBPs/SAM/ITO electrodes showed oxidation peak potential at 0.27 V and reduction peak potential at 0.0006V. These increase and decrease in oxidation and reduction peak potentials, were attributed to high electrocatalytic activity and electrical conductivity of AuNBPs which improve the electron transfer rate between electrode and the electrolyte redox probe [24].…”

Section: Electrochemical Characterizationmentioning

confidence: 95%

Gold nanobipyramids integrated ultrasensitive optical and electrochemical biosensor for Aflatoxin B1 detection

Bhardwaj

Sumana

Marquette

2021

Talanta

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This work reports the development of an electrical and optical biosensing for label-free detection of Aflatoxin B 1 (AFB 1) using gold (Au) nanobipyramids (NBPs). AuNBPs were synthesized through a two-step seed-mediated growth process followed by an exchange of capping agent from surfactant to lipoic acid. Pure and monodispersed AuNBPs of 70 nm base length were obtained and deposited on indium tin oxide (ITO)-coated glass substrate modified with self-assembled (3-Aminopropyl) triethoxysilane (APTES) film. The characterization of the obtained surfaces using spectroscopy, microscopy and diffractometry confirms the formation of AuNBPs, the conjugation to ITO electrode substrate and the immobilization of anti-AFB 1 antibodies. AuNBPs modified ITO substrates were used for both electrochemical and Surface Plasmon Resonance biosensing studies. Localized Surface Plasmon Resonance (LSPR) local field enhancement was demonstrated. SPR based AFB 1 detection was found to be linear in the 0.1 to 500 nM range with a limit of detection of 0.4 nM, whereas, impedimetric AFB 1 detection was shown to be linear in the 0.1 to 25 nM range with a limit of detection of 0.1 nM. The practical utility of the impedimetric sensor was tested in spiked maize samples and 95-100% recovery percentage were found together with low relative standard deviation, proof of the robustness of this AFB 1 sensor.

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Section: Electrochemical Characterizationmentioning

confidence: 95%

Gold nanobipyramids integrated ultrasensitive optical and electrochemical biosensor for Aflatoxin B1 detection

Bhardwaj

Sumana

Marquette

2021

Talanta

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“…The semicircle part at higher frequencies reflected the electron-transfer limited process and the linear portion at lower frequencies corresponded to the diffusionlimited process. The semicircle diameter indicated the charge transfer resistance (Rct) [35]. Here, the semicircle diameters of different coatings followed such order as: P(BT) < P Figure 4B shows the FTIR spectra of P(3-MeT), P(BT), P(BT-3-MeT), rGO, and P(BT-3-MeT)/rGO coatings.…”

Section: Characterization Of Poly(22-bithiopheneco-3-methylthiophenementioning

confidence: 95%

Graphene‐doped electrochemical copolymer coating of 2,2‐bithiophene and 3‐methylthiophene for the highly effective solid‐phase microextraction of volatile benzene homologues

Guo

Zhao

Zeng

2018

J of Separation Science

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We report the electrochemical fabrication of a poly(2,2-bithiophene-co-3-methylthiophene)-graphene composite coating and its application in the headspace solid-phase microextraction and gas chromatography determination of benzenes (i.e., bromobenzene, 4-bromotoluene, 2-nitrotoluene, 3-nitrotoluene and 1,2,4-trichlorobenzene). The coating was uniform and showed cauliflower-like microstructure. It had high thermal stability (up to 375°C) and could be used for at least 180 times of solid-phase microextraction without a decrease in extraction performance. Furthermore, it presented high extraction capacity for the benzenes due to the hydrophobic effect and π-π interaction between the analytes and the coating. Under optimized extraction conditions, good linearity (correlation coefficients higher than 0.9946), wide linear range (0.01-50 μg/L), and low limits of detection (5.25-12.5 ng/L) were achieved for these analytes. The relative standard deviation was lower than 5.7% for five successive measurements with one fiber, and the relative standard deviation for fiber-to-fiber was 4.9-6.8% (n = 5). The solid-phase microextraction and gas chromatography method was successfully applied for the determination of three real samples, and the recoveries for standards added were 89.6-106% for nail polish, 85.8-110% for hair dye, and 90-106.2% for correction fluid, respectively.

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“…Additionally, for sustainability and cost of studies, practical and appropriate cost immunosensors were important criteria in our study. Immunosensors are biosensors that bind to a specific antigen‐antibody . Antibodies are structures produced by the immune system against foreign material or organisms entering the body (bacteria, protozoa and virus).…”

Section: Introductionmentioning

confidence: 92%

A High Sensitive, Reproducible and Disposable Immunosensor for Analysis of SOX2

Tarimeri

Sezgintürk

2020

Electroanalysis

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In this study, an ITO (indium tin oxide) based biosensor was constructed to detect SOX2. SOX2 helps the regulation of cell pluripotency and is closely related to early embryonic development, neural and sexual differentiation. SOX2 is amplified and overexpressed in some malignant tumors such as squamous cell, lung, prostate, breast, esophageal cell, colon, ovarian, glioblastoma, pancreatic cancer, gastric cancer, head and neck squamous cell carcinoma. To generate a hydroxylated clean electrode surface, ITO electrodes were treated with NH4OH/H2O2/H2O. Later, ITO‐PET electrode surfaces were modified with 3‐glycidoxypropyl trimethoxysilane (3‐GOPS). Then, Anti‐SOX2 was covalently immobilized onto the electrode surfaces. 3‐GOPS concentration, Anti‐SOX2 concentration and incubation time, SOX2 incubation time were optimized. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were utilized in order to follow up the immobilization processes and the optimization steps of the biosensor. To characterize the analytical properties of constructed immunosensor; linear range, repeatability, reproducibility and regeneration studies were investigated. The linear range of the immunosensor was detected as 0.625 pg/mL–62.5 pg/mL. Square wave voltammetry technique was also applied to the biosensor. Storage life of the biosensor was determined for identifying the possible usability of the biosensor in clinical field. Finally, the designed biosensor was applied to the real human serum samples. The results obtained with the presented biosensor were also compared with ELISA results.

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AuNPs modified, disposable, ITO based biosensor: Early diagnosis of heat shock protein 70

Cited by 60 publications

References 35 publications

Gold nanobipyramids integrated ultrasensitive optical and electrochemical biosensor for Aflatoxin B1 detection

Gold nanobipyramids integrated ultrasensitive optical and electrochemical biosensor for Aflatoxin B1 detection

Graphene‐doped electrochemical copolymer coating of 2,2‐bithiophene and 3‐methylthiophene for the highly effective solid‐phase microextraction of volatile benzene homologues

A High Sensitive, Reproducible and Disposable Immunosensor for Analysis of SOX2

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