A novel encapsulation technique for the production of artificial seeds

Patel, Anant; Pusch, I.; Mix-Wagner, Gunda; Vorlop, Klaus‐Dieter

doi:10.1007/s002990000223

Cited by 50 publications

(56 citation statements)

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“…The synthetic seed technology which involves the encapsulation of small sized plant propagules with protective nutrient gel provides another promising option for mass propagation because of higher scale up capacity, possibility of automation of whole product process and direct delivery to field. Though synthetic seeds have been developed for potato (Patel et al, 2000), apple (Piccioni, 1997), Catharanthus roseus (Maqsood et al, 2012), banana (Hassanein et al, 2011), eggplant (Huda et al, 2007), black pepper (Nair and Gupta, 2007) and orchid (Mohanraj et al, 2009;Nagananda et al, 2011), reports on synthetic seed production in tomato are very scarce. The present study was conducted with an aim of establishing efficient and reproducible in vitro regeneration protocol for large scale propagation of tomato through seeds and shoot-tip culture.…”

Section: Introductionmentioning

confidence: 99%

Establishment of Efficient in vitro Culture and Plantlet Generation of Tomato (Lycopersicon esculentum Mill.) and Development of Synthetic Seeds

Oceania¹,

Doni²,

Tikendra³

et al. 2014

J. of Plant Sciences

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Tomato is a highly popular crop for its rich nutritional values and constitutes an integral part of daily diets for substantial portion of human population of the world. Because of limitations in conventional breeding methods and increasing demand of this important crop for fast growing population, large scale propagation of tomato through plant tissue culture techniques becomes highly significant. Efficient in vitro generation protocols for tomato have been established successfully by employing seed and tissue culture derived shoot-tip explants. 6-benzyl amino purine (BAP) was highly effective in inducing seed germination and rapid leaf multiplication though presence of 0.4% activated charcocal (AC) had negative influence on culture development. The used of in vitro derived shoot-tips as explants removed the deleterious effect of AC resulting in the production of highest number of leaves in MS+3 mg LG NAA generated highest root formation when shoot-tip propagules were used for culture initiation. Preliminary investigation of synthetic seed development of tomato revealed the gel matrix produced from the complexation between 2% sodium alginate and 100 mM CaCl 2 for 20-40 min as the most favourable for efficient encapsulation of in vitro derived shoot-tip propagules. The in vitro protocols developed from the present investigation can be utilized for propagation of tomato in larger scale for commercial purposes as well as local domestic consumption.

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Section: Introductionmentioning

confidence: 99%

Establishment of Efficient in vitro Culture and Plantlet Generation of Tomato (Lycopersicon esculentum Mill.) and Development of Synthetic Seeds

Oceania¹,

Doni²,

Tikendra³

et al. 2014

J. of Plant Sciences

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“…From this point of view, encapsulation technology has provided a new understanding in recent years, because of its wide use in germplasm conservation and delivery of tissuecultured plants for commercial and research purposes. Calcium alginate hollow beads are used frequently for plant cells, shoot tips, nodal segments, or somatic embryos for conservation purposes, and especially for synthetic seed production (Patel et al, 2000;Rai et al, 2009;Benelli et al, 2013;Sharma, 2013;Kocak et al, 2014). Synthetic seed production in Physalis might also provide year-round productivity, because most plants produce seeds only during certain months of the year.…”

Section: Introductionmentioning

confidence: 99%

Clonal propagation and synthetic seed production from nodal segments ofCape gooseberry (Physalis peruviana L.), a tropical fruit plant

Yücesan

Mohammed²,

Arslan³

et al. 2015

Turk J Agric For

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IntroductionPhysalis peruviana L. is a species from the family Solanaceae and genus Physalis, commonly known as Cape gooseberry (also known as physalis, golden berry, etc.). It is an herbaceous, semishrub, perennial plant, native to tropical Peru and other warm temperate and subtropical regions throughout the world (Ramirez et al., 2013). Cape gooseberry resembles tomato in flavor and appearance, though the sour and sweet taste is richer in this tropical fruit. This plant has increasing popularity due to its nutritional and medicinal values. Cape gooseberry is a source of health-related compounds found in the fruits and other parts of the plant, including leaves and stems. It has been widely used for treating diabetes, hepatitis, ulcers, and several other diseases (Mayorga et al., 2002;Arun and Asha, 2007). It also contains high levels of vitamins A, C, and B-complex, as well as compounds with antiinflammatory and antioxidant properties (Strik, 2007). Wu et al. (2009) reported that supercritical carbon dioxide extracts of P. peruviana leaves induced cell cycle arrest and apoptosis in human lung cancer cells. Similarly, Çakir et al. (2014) reported that phenolic content of ethanolic leaf extracts (100 µg mL -1 ) possessed high cytotoxic effects on HeLa cells (an immortal cell line).The use of tissue culture methods for the selection of the best cultivars for agricultural practices and clonal propagation might be conducive for micropropagation, especially when taking into account high consumption due to value-added natural compounds, limited plant production, and requirements of manpower, transport, and storage (Rodrigues et al., 2013a). Though P. peruviana was reported as recalcitrant for shoot organogenesis through adventitious regeneration from leaf explants (Torres, 1991), there are a limited number of in vitro regeneration studies concerning the micropropagation of P. peruviana (Rodrigues et al., 2013b), not containing information about the synergistic effects of plant growth regulator (PGR) combinations on growth parameters. Several studies on in vitro propagation of other Physalis species, including P. minima, P. ixocarpa, and P. pruinosa,

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“…Somatic embryogenesis is a process having several advantages over organogenesis, such as the probable single cell origin, which reduces somaclonal variations in several geophytes and is applicable for medium-or long-term storage in sodium alginate beads (Ballester et al, 1997;Patel et al, 2000;Gurel and Gurel, 2014). Heavy collection of bulbs from their natural habitats and their use for breeding propagation purposes could have negative impacts on the plant species.…”

Section: Discussionmentioning

confidence: 99%

“…Encapsulation technology provides easy handling of living materials; new understanding towards the storability, uniformity, and the use of sowing equipment; and a barrier against the transmission of pathogens (Patel et al, 2000;Winkelmann et al, 2003). This study describes a simple and effective protocol for both in vitro direct and indirect somatic embryo production from scale explants for subsequent bulblet formation followed by encapsulation of immature bulblets for conservation and storage purposes without disturbing the natural habitats for the first time in an ornamental species.…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and encapsulation of immature bulblets of an ornamental species, grape hyacinths (Muscari armeniacum Leichtlin ex Baker)

Yücesan¹,

Çiçek²,

Gürel³

2014

Turk J Agric For

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An efficient in vitro regeneration system was developed for the ornamental Muscari armeniacum on Linsmaier and Skoog basal medium (LS) supplemented with benzyladenine (BA) at varying concentrations (0.5, 1.0, or 2.0 mg/L) alone or in combination with 0.5 mg/L α-naphthalene acetic acid (NAA). The highest mean number of direct somatic embryo formations was observed on LS medium containing 2.0 mg/L BA and 0.5 mg/L NAA, with a mean of 7.9 somatic embryos per explant after 10 weeks of culture. Green nodular calli induced on LS medium containing 5.0 mg/L BA alone or in combination with 0.5 mg/L NAA were transferred to LS medium supplemented with or without 0.5 mg/L gibberellic acid (GA3) for 8 weeks, producing 23.3 immature bulblets. Immature bulblets produced in vitro were either embedded in a sodium alginate matrix for the encapsulation process or were transferred directly to LS medium supplemented with or without GA 3 at 0.5 or 1.0 mg/L for growth and development for 6 weeks. Encapsulated bulblets were then stored at 4 °C in darkness for 10 weeks and almost all encapsulated bulblets retained their viability and resumed their growth under nonaxenic greenhouse conditions.

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A novel encapsulation technique for the production of artificial seeds

Cited by 50 publications

References 0 publications

Establishment of Efficient in vitro Culture and Plantlet Generation of Tomato (Lycopersicon esculentum Mill.) and Development of Synthetic Seeds

Establishment of Efficient in vitro Culture and Plantlet Generation of Tomato (Lycopersicon esculentum Mill.) and Development of Synthetic Seeds

Clonal propagation and synthetic seed production from nodal segments ofCape gooseberry (Physalis peruviana L.), a tropical fruit plant

Somatic embryogenesis and encapsulation of immature bulblets of an ornamental species, grape hyacinths (Muscari armeniacum Leichtlin ex Baker)

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