Clonal propagation and synthetic seed production from nodal segments ofCape gooseberry (Physalis peruviana L.), a tropical fruit plant

Abstract

IntroductionPhysalis peruviana L. is a species from the family Solanaceae and genus Physalis, commonly known as Cape gooseberry (also known as physalis, golden berry, etc.). It is an herbaceous, semishrub, perennial plant, native to tropical Peru and other warm temperate and subtropical regions throughout the world (Ramirez et al., 2013). Cape gooseberry resembles tomato in flavor and appearance, though the sour and sweet taste is richer in this tropical fruit. This plant has increasing popularity due to its nutritional and medicinal values. Cape gooseberry is a source of health-related compounds found in the fruits and other parts of the plant, including leaves and stems. It has been widely used for treating diabetes, hepatitis, ulcers, and several other diseases (Mayorga et al., 2002;Arun and Asha, 2007). It also contains high levels of vitamins A, C, and B-complex, as well as compounds with antiinflammatory and antioxidant properties (Strik, 2007). Wu et al. (2009) reported that supercritical carbon dioxide extracts of P. peruviana leaves induced cell cycle arrest and apoptosis in human lung cancer cells. Similarly, Çakir et al. (2014) reported that phenolic content of ethanolic leaf extracts (100 µg mL -1 ) possessed high cytotoxic effects on HeLa cells (an immortal cell line).The use of tissue culture methods for the selection of the best cultivars for agricultural practices and clonal propagation might be conducive for micropropagation, especially when taking into account high consumption due to value-added natural compounds, limited plant production, and requirements of manpower, transport, and storage (Rodrigues et al., 2013a). Though P. peruviana was reported as recalcitrant for shoot organogenesis through adventitious regeneration from leaf explants (Torres, 1991), there are a limited number of in vitro regeneration studies concerning the micropropagation of P. peruviana (Rodrigues et al., 2013b), not containing information about the synergistic effects of plant growth regulator (PGR) combinations on growth parameters. Several studies on in vitro propagation of other Physalis species, including P. minima, P. ixocarpa, and P. pruinosa,

Analysis of metabolic variations throughout growth and development of adventitious roots in Silybum marianum L. (Milk thistle), a medicinal plant

“…Generally, sucrose is the most frequently used carbohydrate acting as a signaling molecule, osmoticum and a source of carbon and energy in culture media for following in vitro morphogenetic processes such as adventitious rooting, callus organogenesis and somatic embryogenesis (Khan et al 2015;Yücesan et al 2015). The amount of tryptophan accumulated at LOG phase was significantly higher than other growth phases during adventitious root culture (Fig.…”

An efficient regeneration system and steviol glycoside analysis of Stevia rebaudiana Bertoni, a source of natural high-intensity sweetener

“…However, in the present study, a detailed SG analysis was performed on both micropropagation-and seed-derived plants of S. rebaudiana. For micropropagation, nodal segments are highly capable of shoot induction under in vitro conditions (Sivaram and Mukundan 2003;Thiyagarajan and Venkatachalam 2012;Singh et al 2014;Yücesan et al 2015). It is also noteworthy that MS medium without any growth regulator was found to be highly practical for direct clonal propagation.…”

Effects of salicylic acid and heat acclimation on thermotolerance and withanolide accumulation under high temperature stress in the Cape gooseberry (Physalisperuviana L.)

“…Surface sterilization of seeds and germination were performed according to a method previously published by Yücesan et al (2015). Nodal segments were used as explants from 30-day germinated seedlings for culture initiation.…”