Establishment of Efficient in vitro Culture and Plantlet Generation of Tomato (Lycopersicon esculentum Mill.) and Development of Synthetic Seeds

Oceania, Chirom; Doni, Thingujam; Tikendra, Leimapokpam; Nongdam, Potshangbam

doi:10.3923/jps.2015.15.24

Cited by 11 publications

(9 citation statements)

References 31 publications

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“…Improvement of the plants in culture іn vіtro can be considered as the reason for this increase in yield. As a clonal microreproduction result, better planting material was received, with better sprout tillering, their leaf cover and accordingly considerable inflorescences quantity, and, as is known (Oceania et al, 2015), intensive second level sprouts development and their dense flowering lead to increase in content of essential oil ketones (Tanasіenko, 1985). From Fejеr et al (2018) article it is known that 0.5 mg/l cytokinin BAP addition increases sprouts quantity de novo.…”

Section: Discussionmentioning

confidence: 99%

Effect of clonal reproduction on quantitative indices and component composition of essential oil of peppermint varieties

Talankova-Sereda

Kolomiets

Likhanov³

et al. 2018

Regul. Mech. Biosyst.

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Quantitative and qualitative composition of essential oils of peppermint breeds Lebedinaya Pesnya, Lubenchanka, Lidiya, Ukrainskaya Perechnaya, Mama, Chornolista was investigated before and after clonal microreproduction by the method of isolated tissues and bodies culture in vitro. Methods of essential oil steam distillation, capillary gas chromatography and statistical analysis were used in the research. It is established that increase in essential oil quantity was observed for peppermint breeds on which reproduction and improvement іn vіtro technology was applied. As a result of clonal microreproduction of peppermint plants in culture іn vіtro on nutrient medium Murasige and Skug, in which the growth regulators 0.75 mg/l of 6-benzylaminopurine, 0.1 mg/l of adenine, 0.05 mg/l of indolil-3-acetic acid and 0.5 mg/l of gibberellins acid were added and virocide Ribavirin in concentration 10 mg/l, improvement was obtained in comparison with vegetatively reproduced plants; increase in essential oil quantity per hectare was established for the following breeds; Chornolista by 54.2%, Lebedinaya pesnya by 38.2%, Ukrainskaya Perechnaya by 36.7%, Mama by 28.5%, Lubenchanka by 17.1% and Lidiya by 11.6%. For oil content the highest indices were noted for Lubenchanka, Mama and Lebedinaya Pesnya peppermint breeds with product yield 4.02%, 3.98% and 3.84% respectively. It was established that the essential oil component composition in non-clonal peppermint plants raw materials and plants-regenerants after culture in vitro is variable depending on breed. Limonene, cineole, menthone, menthofuran, iso-menthone, menthyl acetate, β-caryophyllene, iso-menthol, menthol, pulegone, germacren, piperitone, carvone were identified in peppermint essential oil. High content of menthol, low content of carvone, piperitone, pulegone (except for Chornolista, Ukrainskaya Perechnaya breeds) and menthofuran (except for Chornolista, Ukrainskaya Perechnaya and Lubenchanka breeds) are characteristic for Ukrainian selection peppermint investigated breeds. A clear tendency to menthol and menthone content ratio increase is observed in plants which were improved in conditions іn vіtro. Pulegone was not detected in essential oil samples of Lebedinaya Pesnya, Lidiya and Mama breeds. Biochemical markers of Lebedinaya Pesnya, Lubenchanka, Mama breeds, which differentiate them within the group of investigated breeds, are higher limonene, piperitone and menthol pool; for Ukrainskaya Perechnaya and Chornolista breeds – pulegone, cineole and menthone; for Lidiya breed – iso-menthone.

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Section: Discussionmentioning

confidence: 99%

Effect of clonal reproduction on quantitative indices and component composition of essential oil of peppermint varieties

Talankova-Sereda

Kolomiets

Likhanov³

et al. 2018

Regul. Mech. Biosyst.

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“…Maximum number of shoots per explant was induced at 2.5 mg/L BAP in comparison KIN used as a sole growth regulator. Similarly, Oceania et al (2015) and Papry et al (2016) have obtained the maximum shoot multiplication through callus cultures on MS medium augmented with 3.0 mg/L and 2.0 mg/L BAP respectively in Solanum lycopersicum. While the addition of low level of auxin (0.5 mg/L IAA) to cytokinin (BAP/KIN) showed the enhancement in the development of shoots per explant from all the concentrations of cytokinins tested.…”

Section: Discussionmentioning

confidence: 88%

High Frequency Callus Mediated Plantlet Regeneration from Different Explants of Ethno-medicinal Plant Turkey Berry (Solanum torvum Sw)

Malothu¹,

Marka²,

Nanna³

2022

JSR

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We report on the influence of different plant growth regulators (PGRs) as well as the type of explants on callus mediated regeneration in Turkey berry/pea egg medicinal plant, S.torvum. The explants viz., hypocotyl (0.4-0.8 cm long), cotyledon (0.6-0.8 cm 2 ) from 3 week old and leaves (1.0 cm 2 ) from 6 week old in vitro grown seedlings were cultured on MS medium with various concentrations (0.5-4.0 mg/L) of BAP/KIN alone and also in combination with 0.5 mg/L NAA/IAA. The calli developed from all the explants have shown the maximum percentage of response (94% leaf and cotyledon, 88% hypocotyl) and highest number of multiple shoots induction (36.2±1.50 leaf, 33.2±0.25 cotyledon and 23.4±0.11 hypocotyl explants) per explant with maximum length of shoots (3.9±0.18 leaf, 4.0±0.11 cotyledon and 3.7±0.19 hypocotyl) at 0.5 mg/L NAA+2.5 mg/L BAP (leaf), 0.5 mg/L IAA+2.5 mg/L BAP (cotyledon) and 0.5 mg/L IAA+3.0 mg/L BAP (hypocotyl) in comparison to different concentrations of cytokinins used alone and as well as in combination with IAA/NAA. The calli induced from cotyledon explants were found to be more potent in inducing high frequency number of shoots per explant among all other explants tested in the present investigations. Cytokinins BAP/KIN alone or in combination with IAA/NAA was found to be more effective in inducing callus mediated shoot regeneration in all the explants of S. torvum. For in vitro rooting, the elongated microshoots were transferred on to root induction medium (RIM) fortified with 0.25-2.0 mg/L NAA/IAA. Maximum percentage of response (90%), average number of roots (18.2±0.03) per micro-shoot with highest length of roots (7.8±0.09 cms) was observed at 1.0 mg/L IAA. In vitro rooted plantlets were acclimatized in the green house and transferred into field. The survival percentage was found to be 90% and the plantlets were found to be normal in morphology, flowering and fruiting. Thus, the regeneration protocol developed in the present investigation can be used for the conservation and genetic transformation experiments in S. torvum, not only as a medicinal plant but also as a model plant, based on its regeneration potentiality.

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“…The plant components are combined with sodium alginate gel (0.5-5.0 % w/v) and then pipette-dropped into a calcium chloride solution (30-100 mM) to form hydrated seeds. As ion exchange takes place, spherical and solid calcium alginate beads containing somatic embryos are formed, resulting in the sodium ions being replaced by calcium ions [20,21].…”

Section: Hydrated Synthetic Seedsmentioning

confidence: 99%

Production of Synthetic Seeds in Vegetable Crops: A Review

Abbas

Mahood

Alhasan

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Over the last few decades, biotechnology research has advanced to the point where it is now possible to improve essential agricultural crops. Synthetic seed manufacturing has opened up new possibilities of in vitro plant propagation technique since it has various practical benefits on a commercial scale for the cultivation of a wide range of agricultural crops. It is one of the most important tools for plant tissue culture breeders and scientists, since it provides significant benefits for massive production of distinct (elite) plant species. Synthetic seeds offer a promising strategy for mass plant production in a way of encapsulation of non-embryonic or embryonic (somatic embryos) tissues in a gel-like matrix, such as shoot buds, shoot tips, microshoots, protocoms and nodal segments. When the strategies minimize the cost of the production of elite plant genotypes, this strategy can be adapted for commercial production. The technology of the synthetic seed necessitates the use of in vitro growth systems in order to produce viable materials that can be converted into plants on a wide scale. The discovery of an artificial seed technology opens up new possibilities of a promising strategy for improving a wide range of commercially significant plant species, including cereals, fruit crops, medicinal plants, and vegetable crops, which we focus on in this review article.

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Establishment of Efficient in vitro Culture and Plantlet Generation of Tomato (Lycopersicon esculentum Mill.) and Development of Synthetic Seeds

Cited by 11 publications

References 31 publications

Effect of clonal reproduction on quantitative indices and component composition of essential oil of peppermint varieties

Effect of clonal reproduction on quantitative indices and component composition of essential oil of peppermint varieties

High Frequency Callus Mediated Plantlet Regeneration from Different Explants of Ethno-medicinal Plant Turkey Berry (Solanum torvum Sw)

Production of Synthetic Seeds in Vegetable Crops: A Review

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