A Novel Conductometric Urea Biosensor with Improved Analytical Characteristic Based on Recombinant Urease Adsorbed on Nanoparticle of Silicalite

Velychko, Taras; Солдаткін, О. О.; Мельник, В. Г.; Марченко, С. В.; Kirdeciler, Salih Kaan; Akata, Burcu; Солдаткин, А. П.; El’skaya, A. V.; Dzyadevych, S. V.

doi:10.1186/s11671-016-1310-3

Cited by 40 publications

(18 citation statements)

References 30 publications

(28 reference statements)

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“…The procedure of enzyme adsorption on silicalite was tested for a number of enzymes—acetylcholinesterase [27], urease [23, 25], recombinant urease [26], and butyrylcholinesterase [27]. Additionally, the effectiveness of this technique has been shown for enzyme biosensors based on pH-sensitive field-effect transistors [28–32].…”

Section: Introductionmentioning

confidence: 99%

A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

et al. 2017

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In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.

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Section: Introductionmentioning

confidence: 99%

A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

et al. 2017

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“…This decrease of the response may be due to the leak of the immobilized enzyme on the chitosan cryogel beads. The general disadvantage of biosensors with adsorbed enzymes was that the enzyme could slowly be released because the bonds between enzymes and supporting immobilization matrices are weak (Velychko et al, 2016). This urea biosensor stability was similar to the amylase enzyme activity that was immobilized using chitosan remained 43.3% at the fifth usage (Zusfahair, Ningsih, Kartika, Fatoni, & Zuliana, 2017).…”

Section: Operational Stabilitymentioning

confidence: 99%

“…Urea in blood or urine is an important marker for the diagnosis of kidney disease. The normal urea levels in serum are about 2.5 mM to 6.7 mM (Saeedfar, Heng, Ling, & Rezayi, 2013), In patients with kidney failure, the urea levels in serum could increase to 50 -150 mM (Velychko et al, 2016). Kidney disease is one of the common chronic diseases in Indonesia.…”

Section: Introductionmentioning

confidence: 99%

Development of Urea Biosensor Based on Immobilized Urease in Chitosan Cryogel

Zusfahair

Ningsih

Lestari

et al. 2019

Molekul

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The development of biosensors using biological components has an important role in detecting the disease early because it has good selectivity and accuracy. In this study, a biosensor which made is a urea biosensor, based on immobilization urease in chitosan using adsorption techniques, to measure urea levels by colorimetric analysis with bromothymol blue (BTB) as an indicator. The purpose of this study was to find out how to measure urea levels using biosensors based on urease immobilization in chitosan and find out the biosensor performance including optimum enzymatic reaction time, linearity, the limit of detection, repetition, and determination of disrupting compounds. The study began with the making of an immobilization supporting matrix using chitosan which was made in the form of cryogel through an ionic gelation process which adsorbs the urease enzyme. Cryogel urease catalyzes the hydrolysis of urea into NH4+ and CO2-. The reaction product was added with the BTB indicator, and the color change formed was measured using a spectrophotometer. The results showed that the performance of urea biosensors was good enough for urea level detection systems by producing enzymatic reaction times at 15 minutes, linearity at 0.9951, detection limit at 0.018 mM, not affected by the addition of 0.05 mM ascorbic acid and 0.4 mM uric acid. This urea biosensor can be used up to 5 repetitions.

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“…Differential pulse or square wave voltammetry is useful for analyzing multiple analytes or endogenous reactive species in the same sample [ 40 ]. Conductometric relies on the measurement of conductivity or resistivity [ 41 ]. A capacitor changes its capacitance when exposed to the desired analyte, which binds to the capacitor’s surface.…”

Section: Fundamental Aspects Of Electrochemistry and Biorecognitiomentioning

confidence: 99%

Recent Advances of Conducting Polymers and Their Composites for Electrochemical Biosensing Applications

Luong

Narayan

Solanki

et al. 2020

JFB

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Conducting polymers (CPs) have been at the center of research owing to their metal-like electrochemical properties and polymer-like dispersion nature. CPs and their composites serve as ideal functional materials for diversified biomedical applications like drug delivery, tissue engineering, and diagnostics. There have also been numerous biosensing platforms based on polyaniline (PANI), polypyrrole (PPY), polythiophene (PTP), and their composites. Based on their unique properties and extensive use in biosensing matrices, updated information on novel CPs and their role is appealing. This review focuses on the properties and performance of biosensing matrices based on CPs reported in the last three years. The salient features of CPs like PANI, PPY, PTP, and their composites with nanoparticles, carbon materials, etc. are outlined along with respective examples. A description of mediator conjugated biosensor designs and enzymeless CPs based glucose sensing has also been included. The future research trends with required improvements to improve the analytical performance of CP-biosensing devices have also been addressed.

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A Novel Conductometric Urea Biosensor with Improved Analytical Characteristic Based on Recombinant Urease Adsorbed on Nanoparticle of Silicalite

Cited by 40 publications

References 30 publications

A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

Development of Urea Biosensor Based on Immobilized Urease in Chitosan Cryogel

Recent Advances of Conducting Polymers and Their Composites for Electrochemical Biosensing Applications

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