A Novel Catechol Oxidase Enzyme Electrode for the Specific Determination of Catechol

Dinçkaya, Erhan; Akyılmaz, Erol; Akgöl, Sinan; Önal, Seçil; Zihnioğlu, Figen; Telefoncu, Azmi

doi:10.1271/bbb.62.2098

Cited by 7 publications

(5 citation statements)

References 9 publications

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“…5). Chemical immobilization of uricase in gelatine support [3,11] or in sepharose beads [27] revealed poorer robustness since activity decreases of 17% and 30% were respectively obtained in shorter time (25 days). The evaluation of the extent of interferences was carried out for different chemical species normally present in human serum by injection of 50 mmol dm À3 of uric acid solutions to which interferents were added in concentrations similar or above those found in human serum samples.…”

Section: Resultsmentioning

confidence: 99%

“…In the first approach the O 2 consumption or the H 2 O 2 formed is usually monitored while in the second the peroxidase consumption of H 2 O 2 produced in the enzymatic oxidation of uric acid is determined. However, the construction procedures described usually requires time consuming enzyme immobilization procedures [3,7,8], are of relative complex preparation or exhibit lack of robustness [4 -7, 9 -11], and are applied to real sample analysis in a manual batch fashion. Moreover the analytical benefits of the above-mentioned preparations is questionable since some of these electrodes are still capable to oxidize another analytes present in serum samples, or exhibit slow responses [3,4] and short useful lifetime [3,7,11].…”

Section: Introductionmentioning

confidence: 99%

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An Inexpensive Biosensor for Uric Acid Determination in Human Serum by Flow-Injection Analysis

Dutra

Moreira²,

Oliveira³

et al. 2004

Electroanalysis

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An inexpensive and easy to construct miniaturized biosensor is described for the determination of uric acid in biological fluids. The amperometric biosensor was prepared by using a carbon paste electrode prepared with uricase from Arthrobacter globiforms and tetracyanoquinodimethane as electron transfer mediator. When incorporated into a flow-injection system it was enabled to perform 50 measurements/h of uric acid in the analytical range of 1 -100 mmol dm À3 with a relative standard deviation of 0.20% (n ¼ 14). The system was applied to human serum samples analysis providing good data correlation with those obtained by the reference spectrophotometric method. A linear relationship AM (mmol dm À3 ) ¼ 1.02 (AE 0.05) SP (mmol dm À3 ) À 0.12 (AE 0.13) was obtained evidencing the absence of significant error. The constructed biosensor was successfully used for at least four months (250 assays) with only a 13% of decrease in the enzymatic activity.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An Inexpensive Biosensor for Uric Acid Determination in Human Serum by Flow-Injection Analysis

Dutra

Moreira²,

Oliveira³

et al. 2004

Electroanalysis

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“…The catalytic properties of laccase and tyrosinase, which have been used in biosensors to assess the TPI in FRSs, are discussed below. Catechol oxidase (EC 1.10.3.1) is rarely used in biosensors [ 74 , 75 ] due to the ortho-phenol specificity of this enzyme [ 76 ]. However, the recent study [ 77 ] argued that Taraxacum officinale catechol oxidase unexpectedly exhibited tyrosinase activity, i.e., it was able not only to oxidize ortho-diphenols (catecholase activity), but also to ortho-oxygenate monophenols (cresolase activity).…”

Section: Phenol Oxidasesmentioning

confidence: 99%

Biosensors Based on Phenol Oxidases (Laccase, Tyrosinase, and Their Mixture) for Estimating the Total Phenolic Index in Food-Related Samples

Tarasov

Stozhko

Bukharinova

et al. 2023

Life

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Plant phenolic compounds demonstrate bioactive properties in vitro and/or in vivo, which creates demand for their precise determination in life sciences and industry. Measuring the concentration of individual phenolic compounds is a complex task, since approximately 9000 plant phenolic substances have been identified so far. The determination of the total phenolic content (TPC) is less laborious and is used for the qualimetric evaluation of complex multicomponent samples in routine analyses. Biosensors based on phenol oxidases (POs) have been proposed as alternative analytical devices for detecting phenolic compounds; however, their effectiveness in the analysis of food and vegetal matrices has not been addressed in detail. This review describes catalytic properties of laccase and tyrosinase and reports on the enzymatic and bienzymatic sensors based on laccase and tyrosinase for estimating the total phenolic index (TPI) in food-related samples (FRSs). The review presents the classification of biosensors, POs immobilization, the functions of nanomaterials, the biosensing catalytic cycle, interference, validation, and some other aspects related to TPI assessment. Nanomaterials are involved in the processes of immobilization, electron transfer, signal formation, and amplification, and they improve the performance of PO-based biosensors. Possible strategies for reducing interference in PO-based biosensors are discussed, namely the removal of ascorbic acid and the use of highly purified enzymes.

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“…Catechol oxidases (o-diphenol oxidases) oxidize odiphenols into the respective o-quinones in oxygen dependent reactions (Figure 2) [40,41]. A pair of coupled cupric ions is present in active site of catechol oxidase rendering them a status as type-3 copper proteins.…”

Section: Characteristics Of Catechol Oxidasementioning

confidence: 99%

Polyphenol oxidase (PPO) based biosensors for detection of phenolic compounds: A Review

2017

J App Biol Biotech

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The present review summarizes the literature on applications and development of polyphenol oxidase-based biosensors for detection of phenolic compounds present in industrial waste waters. Phenolic compounds including phenol and its derivatives: bisphenol A, catechol, and cresol are widely used in industrial processes. These compounds cause toxicity to living organisms and can be bioaccumulated in environment and food chain. Global production of phenolic compounds is about 50,000 tons per annum. The presence of these compounds in air, water, and food poses toxicity risks to human health and environment. Monitoring of concentration of phenolic compounds is necessary to avoid the risks posed by these compounds. Conventional methods for the detection and quantification of these compounds include laboratory-based spectrophotometric and chromatographic methods. Biosensors can be an efficient alternative to conventional methods due to their inherent specificity, simplicity and quick responsiveness. Biosensors can play an important role to improve the quality of life. Polyphenol oxidasebased biosensors can potentially be applied to detect phenolic compounds in various biological and non-biological materials.

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A Novel Catechol Oxidase Enzyme Electrode for the Specific Determination of Catechol

Cited by 7 publications

References 9 publications

An Inexpensive Biosensor for Uric Acid Determination in Human Serum by Flow-Injection Analysis

An Inexpensive Biosensor for Uric Acid Determination in Human Serum by Flow-Injection Analysis

Biosensors Based on Phenol Oxidases (Laccase, Tyrosinase, and Their Mixture) for Estimating the Total Phenolic Index in Food-Related Samples

Polyphenol oxidase (PPO) based biosensors for detection of phenolic compounds: A Review

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