An inexpensive and easy to construct miniaturized biosensor is described for the determination of uric acid in biological fluids. The amperometric biosensor was prepared by using a carbon paste electrode prepared with uricase from Arthrobacter globiforms and tetracyanoquinodimethane as electron transfer mediator. When incorporated into a flow-injection system it was enabled to perform 50 measurements/h of uric acid in the analytical range of 1 -100 mmol dm À3 with a relative standard deviation of 0.20% (n ¼ 14). The system was applied to human serum samples analysis providing good data correlation with those obtained by the reference spectrophotometric method. A linear relationship AM (mmol dm À3 ) ¼ 1.02 (AE 0.05) SP (mmol dm À3 ) À 0.12 (AE 0.13) was obtained evidencing the absence of significant error. The constructed biosensor was successfully used for at least four months (250 assays) with only a 13% of decrease in the enzymatic activity.
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