Polyphenol oxidase (PPO) based biosensors for detection of phenolic compounds: A Review

doi:10.7324/jabb.2017.50313

Cited by 15 publications

(16 citation statements)

References 116 publications

(156 reference statements)

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“…Tyrosinases catalyze the ortho-hydroxylation of monophenols (monophenolase activity) and the following diphenolase reaction. The advantages of PPOs, such as the ability to catalyze reactions without additional cofactors and to use dissolved oxygen for the oxidation of phenolic compounds, are widely used for PPO analytical applications [2]. In recent years, the interest in using plant (e.g., banana, sweet potato eggplant, avocado) tissues and crude extracts such as PPO as a source for analytical purposes has been observed [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Agaricus bisporus Crude Extract: Characterization and Analytical Application

et al. 2020

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In the present work crude Agaricus bisporus extract (ABE) has been prepared and characterized by its tyrosinase activity, protein composition and substrate specificity. The presence of mushroom tyrosinase (PPO3) in ABE has been confirmed using two-dimensional electrophoresis, followed by MALDI TOF/TOF MS-based analysis. GH27 alpha-glucosidases, GH47 alpha-mannosidases, GH20 hexosaminidases, and alkaline phosphatases have been also detected in ABE. ABE substrate specificity has been studied using 19 phenolic compounds: polyphenols (catechol, gallic, caffeic, chlorogenic, and ferulic acids, quercetin, rutin, dihydroquercetin, l-dihydroxyphenylalanine, resorcinol, propyl gallate) and monophenols (l-tyrosine, phenol, p-nitrophenol, o-nitrophenol, guaiacol, o-cresol, m-cresol, p-cresol). The comparison of ABE substrate specificity and affinity to the corresponding parameters of purified A. bisporus tyrosinase has revealed no major differences. The conditions for spectrophotometric determination have been chosen and the analytical procedures for determination of 1.4 × 10−4–1.0 × 10−3 M l-tyrosine, 3.1 × 10−6–1.0 × 10−4 M phenol, 5.4 × 10−5–1.0 × 10−3 M catechol, 8.5 × 10−5–1.0 × 10−3 M caffeic acid, 1.5 × 10−4–7.5 × 10−4 M chlorogenic acid, 6.8 × 10−5–1.0 × 10−3 M l-DOPA have been proposed. The procedures have been applied for the determination of l-tyrosine in food supplements, l-DOPA in synthetic serum, and phenol in waste water from the food manufacturing plant. Thus, we have demonstrated the possibility of using ABE as a substitute for tyrosinase in such analytical applications, as food supplements, medical and environmental analysis.

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Section: Introductionmentioning

confidence: 99%

Agaricus bisporus Crude Extract: Characterization and Analytical Application

et al. 2020

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“…Enzymatic systems, including biosensors, are an efficient analytical tool for phenolic compounds determination having such advantages as selectivity, sensitivity, reproducibility, high sample throughput, and simplicity in use. Polyphenol oxidase (PPO) demonstrates some advantages over other enzymes: the ability to catalyze oxidation reaction without any additional cofactors, oxidation of phenolic compounds in the presence of oxygen and good stability [11,12]. PPO is a bifunctional enzyme that catalyzes two different reactions: cresolase activity (the hydroxylation of monophenols to o -diphenols) and catecholase activity (the oxidation of o -diphenols to o -quinones).…”

Section: Introductionmentioning

confidence: 99%

“…PPO is a bifunctional enzyme that catalyzes two different reactions: cresolase activity (the hydroxylation of monophenols to o -diphenols) and catecholase activity (the oxidation of o -diphenols to o -quinones). PPO is actively used for creating various biosensors for phenolic compounds determination, these biosensors show high selectivity, sensitivity, good stability and short reaction times [11].…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric and Smartphone-Assisted Determination of Phenolic Compounds Using Crude Eggplant Extract

2019

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In order to develop a simple, reliable and low cost enzymatic method for the determination of phenolic compounds we studied polyphenol oxidase activity of crude eggplant (S. melongena) extract using 13 phenolic compounds. Catechol, caffeic and chlorogenic acids, and l-DOPA have been rapidly oxidized with the formation of colored products. Monophenolic compounds have been oxidized at a much slower speed. Ferulic acid, quercetin, rutin, and dihydroquercetin have been found to inhibit polyphenol oxidase activity of crude eggplant extract. The influence of pH, temperature, crude eggplant extract amount, and 3-methyl-2-benzothiazolinone hydrazone (MBTH) concentration on the oxidation of catechol, caffeic acid, chlorogenic acid, and l-DOPA has been investigated spectrophotometrically. Michaelis constants values decrease by a factor of 2 to 3 in the presence of MBTH. Spectrophotometric (cuvette and microplate variants) and smartphone-assisted procedures for phenolic compounds determination have been proposed. Average saturation values (HSV color model) of the images of the microplate wells have been chosen as the analytical signal for smartphone-assisted procedure. LOD values for catechol, caffeic acid, chlorogenic acid, and l-DOPA equaled 5.1, 6.3, 5.8 and 30.0 µM (cuvette procedure), 12.2, 13.2, 13.2 and 80.4 µM (microplate procedure), and 23.5, 26.4, 20.8 and 120.6 µM (smartphone procedure). All the variants have been successfully applied for fast (4-5 min) and simple TPC determination in plant derived products and l-DOPA determination in model biological fluids. The values found with smartphone procedure are in good agreement with both spectrophotometric procedures values and reference values. Using crude eggplant extract- mediated reactions combined with smartphone camera detection has allowed creating low-cost, reliable and environmentally friendly analytical method for the determination of phenolic compounds.

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“…The choice of support material is essential for conferring stability, selectivity and even improve enzyme activity. Consequently, the support material must be inert, stable, and resistant [ 27 ]. The immobilization technique is highly significant; without immobilization, the enzyme cannot be stable and reusable.…”

Section: Enzyme Based Biosensorsmentioning

confidence: 99%

Enzyme (Single and Multiple) and Nanozyme Biosensors: Recent Developments and Their Novel Applications in the Water-Food-Health Nexus

et al. 2021

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The use of sensors in critical areas for human development such as water, food, and health has increased in recent decades. When the sensor uses biological recognition, it is known as a biosensor. Nowadays, the development of biosensors has been increased due to the need for reliable, fast, and sensitive techniques for the detection of multiple analytes. In recent years, with the advancement in nanotechnology within biocatalysis, enzyme-based biosensors have been emerging as reliable, sensitive, and selectively tools. A wide variety of enzyme biosensors has been developed by detecting multiple analytes. In this way, together with technological advances in areas such as biotechnology and materials sciences, different modalities of biosensors have been developed, such as bi-enzymatic biosensors and nanozyme biosensors. Furthermore, the use of more than one enzyme within the same detection system leads to bi-enzymatic biosensors or multi-enzyme sensors. The development and synthesis of new materials with enzyme-like properties have been growing, giving rise to nanozymes, considered a promising tool in the biosensor field due to their multiple advantages. In this review, general views and a comparison describing the advantages and disadvantages of each enzyme-based biosensor modality, their possible trends and the principal reported applications will be presented.

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Polyphenol oxidase (PPO) based biosensors for detection of phenolic compounds: A Review

Cited by 15 publications

References 116 publications

Agaricus bisporus Crude Extract: Characterization and Analytical Application

Agaricus bisporus Crude Extract: Characterization and Analytical Application

Spectrophotometric and Smartphone-Assisted Determination of Phenolic Compounds Using Crude Eggplant Extract

Enzyme (Single and Multiple) and Nanozyme Biosensors: Recent Developments and Their Novel Applications in the Water-Food-Health Nexus

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