A novel antibacterial biomaterial mesh coated by chitosan and tigecycline for pelvic floor repair and its biological performance

Abstract

The biomaterials composed of mammalian extracellular matrix (ECM) have a great potential in pelvic floor tissue repair and functional reconstruction. However, bacterial infection does cause great damage to the repair function of biomaterials which is the major problem in clinical utilization. Therefore, the development of biological materials with antimicrobial effect is of great clinical significance for pelvic floor repair. Chitosan/tigecycline (CS/TGC) antibacterial biofilm was prepared by coating CS/TGC nanoparticles on mammalian-derived ECM. Infrared spectroscopy, scanning electron microscopy, bacteriostasis circle assay and static dialysis methods were used to characterize the membrane. MTS assay kit and DAPI fluorescence staining were used to evaluate cytotoxicity and cell adhesion. The biocompatibility was assessed by subabdominal implantation model in goats. Subcutaneous antimicrobial test in rabbit back was used to evaluate the antimicrobial and repairing effects on the infected wounds in vivo. Infrared spectroscopy showed that the composite coating had been successfully modified. The antibacterial membrane retained the main structure of ECM multilayer fibers. In vitro release of biomaterials showed sustained release and stability. In vivo studies showed that the antibacterial biological membrane had low cytotoxicity, fast degradation, good compatibility, anti-infection and excellent repair ability.

Chitooligosaccharides Derivatives Protect ARPE-19 Cells against Acrolein-Induced Oxidative Injury

“…For the FT-IR spectra ( Figure 1 D), the bands at 3370 cm −1 , 2876 cm −1 , and 1073 cm −1 were corresponded to stretching vibrations of O-H, C-H and C-O, respectively. The spectra of N -acetyl chitosan oligosaccharides showed the characteristic absorptions of 1649 cm −1 , 1549 cm −1 and 1314 cm −1 , which were attributed to amide I, II and III bands of amide, respectively [ 30 ]. Moreover, there was no 1735 cm −1 band (-C(=O)O-) in the N -acetylated chitosan oligosaccharide, indicating no acetylation on the OH groups of COSs.…”

Recent advances in adhesive materials used in the biomedical field: adhesive properties, mechanism, and applications

“…178,179 Desirable biological functions can be achieved by combining and utilizing different adhesive materials. 179 CS can promote Schwann cell growth, 41 pelvic floor tissue repair, 180 and 3D tissue growth, and is often used to make skin tissue regeneration, antibacterial, and anti-oxidation multi-functional hydrogels. CS-Based multifunctional hydrogels can achieve rapid in situ gelation at the wound site and completely cover irregular wounds, induce skin tissue remodeling, promote vascular repair and hair follicle regeneration, and accelerate wound healing.…”

Hemocompatibility Multi-in-One Hydrogel Coating with ROS-Triggered Inflammation Suppression and Anti-Infection Properties for Blood-Contacting Device

“…12−14 Once the biofilm forms, the equipment has to be replaced, and serious infections can even cause the death of the patient. 15,16 Finally, since the body functions of patients are weaker and more complex than those of people without diseases, the body's inflammatory response to medical devices is almost inevitable. Inflammation will further aggravate a patient's disease and might result in a variety of other diseases.…”

“…Blood-contacting medical devices have been widely used clinically to save the lives of countless patients, such as central venous catheters (CVCs), extracorporeal circulation equipment, artificial hearts, and venous filters. − These devices might also cause a series of complications, including thrombosis, infection, inflammation, and so on. − When blood contacts the device, a cascade of events are initiated, such as protein adsorption and platelet and fibrin activation. These events may result in the formation of thrombus on the device surface. , Thrombus causes device failure; the detached thrombus can also cause blood vessel blockage, induce cerebral infarction, pulmonary embolism, and so on. ,, On the other hand, medical devices with long-term implantation (especially percutaneous implantation) are prone to induce bacterial adhesion on the surface, and further forming the biofilm, making antibiotics ineffective. − Once the biofilm forms, the equipment has to be replaced, and serious infections can even cause the death of the patient. , Finally, since the body functions of patients are weaker and more complex than those of people without diseases, the body’s inflammatory response to medical devices is almost inevitable. Inflammation will further aggravate a patient’s disease and might result in a variety of other diseases …”