A new HPLC method for azithromycin quantitation

Zubata, Patricia; Ceresole, Rita; Rosasco, María Ana; Pizzorno, M. T.

doi:10.1016/s0731-7085(01)00554-4

Cited by 55 publications

(25 citation statements)

References 4 publications

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“…6 However, the disadvantages of this method are time-consuming and cumbersome, many impact factors and bigger error etc. According to reported, liquid chromatography, [7][8][9][10][11] reverse liquid phase chromatography, 12 UV irradiation 13,14 and liquid chromatography-mass spectrometry (LC-MS) [15][16][17] were used to analyse azithromycin and its related substances. Owing to no conjugated double bonds in the chemical structure of azithromycin, the end absorption just existed in the vicinity of 190-200 nm wavelength.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Application of Electrochemiluminescence Sensor by Immobilizing Tris(2, 2′-bipyridine) ruthenium(II) on the Surface of Gold Electrode with Silica sol/Nano-Au/PVP/<i>L</i>-cysteine

Luo

et al. 2015

Electrochemistry

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In order to establish a simple, sensitive and rapid method for the determination of azithromycin, the gold electrode modified with Silica sol/Nano-Au/PVP/Ru(bpy) 3 2+ was prepared, and the electrochemiluminescence (ECL) method was developed for the determination of azithromycin based on the sensitizing effect of azithromycin on Ru(bpy) 3 2+. Moreover, the electrochemical behaviors and the electrochemical luminescence behaviors of the Ru(bpy) 3 2+ system and the Ru(bpy) 3 2+-azithromycin system were investigated by ECL respectively. The results show that the proposed electrode showed very good electrochemical activity and electrochemiluminescence response to the Ru(bpy) 3 2+-azithromycin system, under the optimum experimental condition, the linearity of azithromycin concentration to ECL intensity was achieved in the concentration of azithromycin from 1.0 × 10 −7 to 1.0 × 10 −4 mol L −1 and the detection limit (S/N = 3) was 1.33 × 10 −9 mol L −1. Through the parallel determination of 1.0 × 10 −5 mol L −1 azithromycin for 8 times, the RSD of results was 1.82%, and the recovery ranging from 97.9% to 101.6% was obtained in the detection of azithromycin tabelets and the RSD was 1.36% (n = 5). The proposed method showed high selectivity and sensitivity and simplicity, and it could be applied to the analysis of azithromycin with satisfactory results.

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Section: Introductionmentioning

confidence: 99%

Preparation and Application of Electrochemiluminescence Sensor by Immobilizing Tris(2, 2′-bipyridine) ruthenium(II) on the Surface of Gold Electrode with Silica sol/Nano-Au/PVP/<i>L</i>-cysteine

Luo

et al. 2015

Electrochemistry

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“…On the other hand, the development of HPLC methods with UV detection is not a common subject because of the extremely low absorption capacity of the azithromycin in this spectral range. Nevertheless, Zubata et al 15 proposed a simple and lowsensitivity methodology for the azithromycin determination in pharmaceuticals using this approach.…”

Section: Introductionmentioning

confidence: 99%

Novel spectrophotometric method for the determination of azithromycin in pharmaceutical formulations based on its charge transfer reaction with quinalizarin

Paula¹,

Almeida²,

Cassella³

2010

J. Braz. Chem. Soc.

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Neste trabalho é proposto um novo método para a determinação espectrofotométrica simples e rápida de azitromicina em formulações farmacêuticas. O método está baseado na reação de transferência de carga entre a azitromicina e a quinalizarina em meio de metanol. O efeito de diversas variáveis foi avaliado com o intuito de alcançar máxima sensibilidade analítica. A reação de transferência de carga foi caracterizada, sendo determinadas a estequiometria da reação, a absortividade molar aparente e a constante de associação. Melhores resultados foram obtidos em meio de metanol e com uma concentração de quinalizarina de 50 mg L -1 . Nestas condições, o ânion radicalar (produto da reação de transferência de carga) foi formado imediatamente após a mistura dos reagentes, apresentando máxima absorção em 564 nm. O método apresentou um limite de detecção de 0,35 mg L -1 e um limite de quantificação de 1,2 mg L -1 , sendo aplicado com sucesso na determinação de azitromicina em três amostras de medicamentos comerciais contendo azitromicina. This paper proposes a new method for simple and fast spectrophotometric determination of azithromycin in pharmaceutical formulations. The method is based on the charge transfer reaction between the azithromycin and quinalizarin in methanol medium. In order to achieve maximum sensitivity the effect of some chemical variables such as the type of solvent, reagent concentration and reaction time were evaluated. The reaction was characterized in terms of stability of the product formed and its stoichiometry, and the apparent molar absorptivity and association constant were derived. Best conditions for the analytical determination of azithromycin were observed in methanol medium with a quinalizarin concentration of 50 mg L -1 . At these conditions, the radical anion (absorbing specie) was formed in the medium immediately after mixing of the reagents and showed maximum absorption at 564 nm. The method presented a limit of detection of 0.35 mg L -1 and a limit of quantification of 1.2 mg L -1 . It was successfully applied in the determination of azithromycin in three commercial pharmaceutical formulations of azithromycin and no matrix interferences were observed.

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“…1,2 It is used in respiratory tract infections, like pharyngitis, pneumonia, chronic bronchitis, bronchopneumonia, skin and soft tissue infections and some sexually transmitted diseases. [2][3][4][5] Several methods have been described in the literature for the determination of AZ in pharmaceutical formulations and biological fluids, including spectrophotometry, 2,6-10 spectrofluorometry, 11 voltammetry, 4,5,12,13 high-performance liquid chromatography using electrochemical, 14,15 amperometric, 16 fluorescence, 17 UV, 18,19 and electrospray ionization mass spectrometry, 20 detection and bioassay. 21,22 Some of these methods display good detection limits and accuracy.…”

Section: Introductionmentioning

confidence: 99%

“…21,22 Some of these methods display good detection limits and accuracy. 5,[11][12][13][14][15][16][17][18]20,22 However, most of them are time-consuming 2,4-9,11 -13,15,17,21,22 and in some cases require expensive equipment. 11,[14][15][16][17][18][19][20] The United States Pharmacopeia 23 proposes a chromatographic method with amperometric detection that needs the use of a specific "Gamma-alumina" column, which is expensive and difficult to obtain commercially.…”

Section: Introductionmentioning

confidence: 99%

Flow-Injection Spectrophotometric Determination of Azithromycin in Pharmaceutical Formulations Using p-Chloranil in the Presence of Hydrogen Peroxide

Rufino

Pezza

2008

ANAL. SCI.

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A flow-injection (FI) spectrophotometric procedure exploiting merging zones is proposed for the determination of azithromycin in pharmaceutical formulations. The method is based on the reaction of azithromycin with tetrachloro-pbenzoquinone (p-chloranil) accelerated by hydrogen peroxide and conducted in a methanol medium, producing a purplered color compound (lmax = 540 nm). The FI system and the experimental conditions were optimized using a multivariate method. Beer's law is obeyed in a concentration range of 50 -1600 mg mL -1 with an excellent correlation coefficient (r = 0.9998). The detection limit and the quantification limit were 6.6 and 22.1 mg mL -1 , respectively. No interference was observed from the common excipients, and the recoveries were within 98.6 to 100.4%. The procedure was applied to the determination of azithromycin in pharmaceuticals with a high sampling rate (65 samples h -1 ). The results obtained by the proposed method were in good agreement with those obtained by the comparative method at 95% confidence level.

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A new HPLC method for azithromycin quantitation

Cited by 55 publications

References 4 publications

Preparation and Application of Electrochemiluminescence Sensor by Immobilizing Tris(2, 2′-bipyridine) ruthenium(II) on the Surface of Gold Electrode with Silica sol/Nano-Au/PVP/<i>L</i>-cysteine

Preparation and Application of Electrochemiluminescence Sensor by Immobilizing Tris(2, 2′-bipyridine) ruthenium(II) on the Surface of Gold Electrode with Silica sol/Nano-Au/PVP/<i>L</i>-cysteine

Novel spectrophotometric method for the determination of azithromycin in pharmaceutical formulations based on its charge transfer reaction with quinalizarin

Flow-Injection Spectrophotometric Determination of Azithromycin in Pharmaceutical Formulations Using p-Chloranil in the Presence of Hydrogen Peroxide

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A new HPLC method for azithromycin quantitation

Cited by 55 publications

References 4 publications

Preparation and Application of Electrochemiluminescence Sensor by Immobilizing Tris(2, 2&prime;-bipyridine) ruthenium(II) on the Surface of Gold Electrode with Silica sol/Nano-Au/PVP/<i>L</i>-cysteine

Preparation and Application of Electrochemiluminescence Sensor by Immobilizing Tris(2, 2&prime;-bipyridine) ruthenium(II) on the Surface of Gold Electrode with Silica sol/Nano-Au/PVP/<i>L</i>-cysteine

Novel spectrophotometric method for the determination of azithromycin in pharmaceutical formulations based on its charge transfer reaction with quinalizarin

Flow-Injection Spectrophotometric Determination of Azithromycin in Pharmaceutical Formulations Using p-Chloranil in the Presence of Hydrogen Peroxide

Contact Info

Product

Resources

About

Preparation and Application of Electrochemiluminescence Sensor by Immobilizing Tris(2, 2′-bipyridine) ruthenium(II) on the Surface of Gold Electrode with Silica sol/Nano-Au/PVP/<i>L</i>-cysteine

Preparation and Application of Electrochemiluminescence Sensor by Immobilizing Tris(2, 2′-bipyridine) ruthenium(II) on the Surface of Gold Electrode with Silica sol/Nano-Au/PVP/<i>L</i>-cysteine