Preparation and Application of Electrochemiluminescence Sensor by Immobilizing Tris(2, 2&prime;-bipyridine) ruthenium(II) on the Surface of Gold Electrode with Silica sol/Nano-Au/PVP/<i>L</i>-cysteine

Abstract

In order to establish a simple, sensitive and rapid method for the determination of azithromycin, the gold electrode modified with Silica sol/Nano-Au/PVP/Ru(bpy) 3 2+ was prepared, and the electrochemiluminescence (ECL) method was developed for the determination of azithromycin based on the sensitizing effect of azithromycin on Ru(bpy) 3 2+. Moreover, the electrochemical behaviors and the electrochemical luminescence behaviors of the Ru(bpy) 3 2+ system and the Ru(bpy) 3 2+-azithromycin system were investigated by ECL respectively. The results show that the proposed electrode showed very good electrochemical activity and electrochemiluminescence response to the Ru(bpy) 3 2+-azithromycin system, under the optimum experimental condition, the linearity of azithromycin concentration to ECL intensity was achieved in the concentration of azithromycin from 1.0 × 10 −7 to 1.0 × 10 −4 mol L −1 and the detection limit (S/N = 3) was 1.33 × 10 −9 mol L −1. Through the parallel determination of 1.0 × 10 −5 mol L −1 azithromycin for 8 times, the RSD of results was 1.82%, and the recovery ranging from 97.9% to 101.6% was obtained in the detection of azithromycin tabelets and the RSD was 1.36% (n = 5). The proposed method showed high selectivity and sensitivity and simplicity, and it could be applied to the analysis of azithromycin with satisfactory results.

Electrochemiluminescence Sensor Based on Electrospun Three-Dimensional Carbon Nanofibers for the Detection of Difenidol Hydrochloride

“…The buffer type and pH value used will greatly determine the stability and signal strength in ECL-based detection systems [48]. Thus, selecting the best buffer solution is vital for the detection of difenidol.…”