A new clean-up approach for stomach content toxicological analysis

Peres, Mariana Dadalto; Nascimento, Simone; Pelição, Fabrício Souza

doi:10.1016/j.forsciint.2019.109936

Cited by 10 publications

(4 citation statements)

References 18 publications

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“…In general, the method described presents a much faster analysis time, a higher precision and similar accuracy to the chromatographic methods found in the literature. However, chromatographic methods generally present better LoDs [ 29 , 30 ].…”

Section: Resultsmentioning

confidence: 99%

Electropolymerization of Metallo-Octaethylporphyrins: A Study to Explore Their Sensing Capabilities

et al. 2022

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The electropolymerization of metallo-octaethylporphyrins (OEP) containing copper, zinc or nickel metal were performed using cyclic voltammetry at three different potential ranges. The electropolymerized porphyrins were characterized by UV-Vis and Raman spectroscopies and the Soret band (393–445 nm) and Raman bands were used to assess the degree of electropolymerization obtained. The application for an analytical use of the modified electrodes to determine phenobarbital in aqueous solution was evaluated. The electropolymerized CuOEP produced at potentials ranging from 0.0 to 2.2 V was the best performer with a limit of detection (LoD) of 10 mg L−1 (43.07 µM), a linear range of 10–150 mg L−1 (43.07 to 646 µM), an average precision of 4.3% (%RSD) and an average % recovery of 101.34%. These results indicate that the CuOEP-modified electrode is suitable for the analysis of phenobarbital in human samples, as the concentration range varies from 10 to 40 mg L−1 (43.07 to 172.27 µM), typically found in antiepileptic treatments, to those at the toxic level (172–258 µM) or lethal levels (345–650 µM).

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Section: Resultsmentioning

confidence: 99%

Electropolymerization of Metallo-Octaethylporphyrins: A Study to Explore Their Sensing Capabilities

et al. 2022

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“…Stomach or gastric content has been used in toxicological analysis and in simple visual examination for tablets, capsules, pill fragments, blotter paper, or other signs of drug use. Depending on the state of degradation, it may be possible to perform a presumptive visible identification of these types of evidence if identifying marks or by analysis by typical analytical techniques (Peres et al, 2019). The analysis of gastric contents can be useful when other specimens are not available for analysis.…”

Section: Gastric Contentsmentioning

confidence: 99%

An overview of sample preparation in forensic toxicology

Jones

McGowan

Boyle

et al. 2021

WIREs Forensic Science

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Sample preparation is an important step in forensic toxicological analysis. With the technological advancements and the availability of mass spectrometers with increasing sensitivities, the need to remove potential interferences, such as matrix components or non‐relevant analytes to the analysis, is essential to optimizing an analytical method. Forensic toxicological analysis involves diverse analytes with a variety of chemical properties that need to be considered when selecting an appropriate sample preparation technique. The push for broad standards and best practice documents for forensic toxicology demonstrates the need for laboratories to ensure they meet the required capabilities to not only detect specific classes of drugs commonly encountered in forensic case work, but also ensure method validity. The objective of this article is to provide the forensic toxicology community a high‐level overview of biological matrices and their relevant components that should be considered during sample pre‐treatment as well as traditional and non‐traditional sample preparation techniques and present general applications. This article is categorized under: Toxicology > Drug Analysis

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“…Arce‐López and colleagues achieved the simultaneous determination of 19 mycotoxins in human plasma through an LC–MS/MS‐based method, due to the great clean‐up efficiency and good recovery of the EMR‐lipid method (Arce‐Lopez, Lizarraga, Flores‐Flores, Irigoyen, & Gonzalez‐Penas, ). Moreover, Peres, Nascimento, and Pelição () established a new GC–MS method, by incorporating an EMR‐lipid method. Their findings demonstrate that the EMR‐lipid method can offer a reliable clean‐up for high lipids samples, and be suitably used for analyzing pesticides and drugs of forensic interests.…”

Section: Sample Clean‐up and Recoverymentioning

confidence: 99%

Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies

Chen

Gao

Zhong

2020

Biomedical Chromatography

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Chromatographic method has long been recognized as the most widely used separation method in bioanalytical research. However, the relatively low sensitivity of existing chromatographic methods remains a significant challenge, as the requirements for experimental procedures become more demanding. This review discusses the main causes for the low sensitivity of chromatographic methods and aims to introduce different technologies for enhancing their sensitivity in the following aspects: (i) different pretreatment methods for improving clean‐up efficiency and recovery; (ii) derivatization step for altering the chromatographic behavior of analytes and enhancing MS ionization efficiency; (iii) optimal LC–MS conditions and appropriate separation mechanism; and (iv) applications of other chromatographic methods, including miniaturized LC, 2D‐LC, 2D‐GC, and supercritical fluid chromatography. Altogether, this review is devoted to summarizing the recent technologies reported in the literature and providing new strategies for the detection of bioanalytes.

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A new clean-up approach for stomach content toxicological analysis

Cited by 10 publications

References 18 publications

Electropolymerization of Metallo-Octaethylporphyrins: A Study to Explore Their Sensing Capabilities

Electropolymerization of Metallo-Octaethylporphyrins: A Study to Explore Their Sensing Capabilities

An overview of sample preparation in forensic toxicology

Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies

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