A Nanohook‐Equipped Bionanocatalyst for Localized Near‐Infrared‐Enhanced Catalytic Bacterial Disinfection

Abstract

Novel bionanocatalysts have opened a new era in fighting multidrug‐resistant (MDR) bacteria. They can kill bacteria by elevating the level of reactive oxygen species (ROS) in the presence of chemicals like H2O2. However, ROSs’ ultrashort diffusion distance limit their bactericidal activity. We present a nanohook‐equipped bionanocatalyst (Ni@Co‐NC) with bacterial binding ability that shows robust ROS‐generating capacity under physiological H2O2 levels. The Ni@Co‐NC's pH‐dependent performance confines its effects to the biofilm microenvironment, leaving healthy tissue unaffected. Furthermore, it can generate heat upon NIR laser irradiation, enhancing its catalytic performance while achieving heat ablation against bacteria. With the Ni@Co‐NC's synergistic effects, bacterial populations fall by >99.99 %. More surprisingly, the mature biofilm shows no recurrence after treatment with the Ni@Co‐NC, demonstrating its tremendous potential for treating MDR bacterial related infections.

Biomimetic electrodynamic nanoparticles comprising ginger-derived extracellular vesicles for synergistic anti-infective therapy

“…After NIR light was applied, elevated levels of ROS were detected in the EV-Pd-Pt + E + L group, which is consistent with the NIR-enhanced catalytic activity of the Pd-Pt nanosheets (Fig. 3a) 42 . These results demonstrate the great potential of EV-Pd-Pt to combat bacterial infections through the synergistic effect of EDT and PTT.…”

Antibacterial Theranostic Agents with Negligible Living Cell Invasiveness: AIE-Active Cationic Amphiphiles Regulated by Alkyl Chain Engineering

“…As shown in Figure 4D, PyBu-PIO is found to localize on the bacterial surface whose hollow interior overlays well with the nucleic acid region labeled by Hoechst 33342, illustrating that PyBu-PIO mainly stains the envelope structures of Gram-positive bacteria, probably involving the cell wall and membranes. 72,73 Satisfactorily, this finding is well expanded to other Gram-positive bacteria strains including S. epidermidis, B. subtilis, and E. faecalis. In addition, when the bacterial envelope is destroyed by 75% ethanol, PyBu-PIO can enter the cytoplasm of both S. aureus and E. coli (Figure 4E), reflecting an intact envelope structure is critical to the selectivity and binding effect of PyBu-PIO to living Grampositive bacteria.…”

Photothermal Therapy with Ag Nanoparticles in Mesoporous Polydopamine for Enhanced Antibacterial Activity

“…Then, the solution was incubated at room temperature. A sample (2 mL) was taken at predetermined points of time (3,6,9,12,24,30,36, and 48 h), replenishing with an equal volume of fresh phosphate buffer each time. The solution was centrifuged (13,000 rpm, 10 min), and the supernatant was filtered with a 0.22 μm membrane to guarantee that the nanoparticles were removed as pellets and Ag + remained in the supernatant.…”

“…Infectious diseases caused by pathogenic bacteria raise great social concern and exert a huge burden on public health and economy. , There is no doubt that antibiotics play an extremely important role in treating bacterial infections, which reduced morbidity and mortality greatly. − However, due to the abuse and overuse of antibiotics in past decades, more and more bacteria have become drug-resistant strains, causing the antibacterial efficiency of many traditional antibiotics to decrease gradually, , and drug-resistant bacterial infections now appear as one of the harsh challenges to public healthcare . Furthermore, another bad news is that the discovery of completely new antibiotics is far behind the evolution of bacterial resistance, posing a serious threat to antibiotic treatments in the world. , Therefore, it is high time that researchers should develop efficient antibacterial strategies to address bacterial infections.…”