Photothermal therapy (PTT) is a promising method to kill pathogenic bacteria. However, using single-modal PTT, it is easy to cause tissue damage because of the overtemperature. Hence, it is necessary to combine PTT with other antibacterial strategies. In this study, we employed silver nanoparticles (Ag NPs) and mesoporous polydopamine (MPDA) nanoparticles to establish a multimodal antibacterial platform (MPDA@Ag). MPDA nanoparticles not only served as a photothermal transduction agent to convert light-energy to heat under the irradiation of near-infrared (NIR) light but also acted as a reducer to reduce Ag NPs in situ. Because Ag NPs can effectively kill bacteria, MPDA@Ag can overcome the drawbacks of the single-modal PTT antibacterial strategy, achieving a synergistic antibacterial effect. Moreover, as an antibiotic-free strategy, MPDA@Ag was less likely to induce drug resistance when killing bacteria. In summary, this platform offered a simple and practical means to achieve excellent antibacterial activity and supplied a good alternative to fight pathogenic bacteria.
Vibrio parahaemolyticus is a common marine foodborne pathogen that causes gastroenteritis. With the long‐term use of antibiotics, many bacteria become resistant; therefore, developing antibiotic‐free antimicrobial strategies is urgent. Epigallocatechin gallate (EGCG), a constituent of polyphenols present abundantly in tea extract, has broad‐spectrum antibacterial activity and is non‐toxic. Here, we take advantage of these properties of EGCG to evaluate its inhibition effect on the growth and biofilm formation of V. parahaemolyticus 17802, and explore its antibacterial mechanism. It was found that EGCG showed antibacterial activity against V. parahaemolyticus 17802, and the minimum inhibitory concentration (MIC) was estimated to be 128 μg ml−1. Results of crystal violet staining and confocal laser scanning microscope (CLSM) evidenced that EGCG hindered its biofilm formation. Moreover, the swimming motility and extracellular polysaccharides were also notably inhibited. The antibacterial mechanism was further confirmed by several assays, such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), and live/dead staining assay, together with membrane permeability assay, which all suggested that EGCG caused damage to cell membrane and made it lose integrity, eventually resulting in the death of V. parahaemolyticus 17802. The bactericidal activity of EGCG verified its potential as a promising candidate to combat foodborne pathogen.
The synthesis of functional antibacterial nanoplatforms for killing pathogenic bacteria is urgent. Among them, combining traditional antibiotics with antimicrobial photodynamic therapy (PDT) is an attractive means. Here, we synthesized a multimodal antibacterial nanoplatform (denoted as Van/PCN) based on . PCN-224 is a kind of photosensitizer-based nanoscale metal organic framework (pMOF), which not only possessed large surface area and many binding sites for loading Van but also had the ability to produce ROS under visible light irradiation. Van/PCN killed bacteria by Van and ROS, achieving a synergistic antibacterial effect. Since Gram-positive bacteria are sensitive to Van, this antibacterial nanoplatform has great potential to treat bacterial infections caused by Gram-positive pathogens.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.