Biomimetic electrodynamic nanoparticles comprising ginger-derived extracellular vesicles for synergistic anti-infective therapy

Abstract

Nanotechnology enlightens promising antibacterial strategies while the complex in vivo infection environment poses a great challenge to the rational design of nanoplatforms for safe and effective anti-infective therapy. Herein, a biomimetic nanoplatform (EV-Pd-Pt) integrating electrodynamic Pd-Pt nanosheets and natural ginger-derived extracellular vesicles (EVs) is proposed. The introduction of ginger-derived EVs greatly endows EV-Pd-Pt with prolonged blood circulation without immune clearance, as well as accumulation at infection sites. More interestingly, EV-Pd-Pt can enter the interior of bacteria in an EV lipid-dependent manner. At the same time, reactive oxygen species are sustainably generated in situ to overcome the limitations of their short lifetime and diffusion distance. Notably, EV-Pd-Pt nanoparticle-mediated electrodynamic and photothermal therapy exhibit synergistic effects. Furthermore, the desirable biocompatibility and biosafety of the proposed nanoplatform guarantee the feasibility of in vivo applications. This proof-of-concept work holds significant promise for developing biomimetic nanoparticles by exploiting their intrinsic properties for synergistic anti-infective therapy.

Semiconducting Titanate Supported Ruthenium Clusterzymes for Ultrasound‐Amplified Biocatalytic Tumor Nanotherapies

“…We characterized the Zeta potentials of the BTO, RuO/BTO, RuNP/BTO, and RuNC/BTO in PBS solution, as shown in Figure S13, Supporting Information; all the samples are negatively charged, ensuring the good dispersion for nanoparticles in blood circulation. [ 62 ] We also explored the dispersibility of RuNC/BTO in different media, thus suggesting good dispersion stability (Figures S14–S16, Supporting Information). In the follow‐up study, the RuNP/BTO and RuO/BTO will be used as the control samples to the RuNC/BTO for comparison.…”

Recent advances of cell membrane-coated nanoparticles for therapy of bacterial infection

“…EV-Pd-Pt got access to the interior of bacteria via an EV lipiddependent means. Remarkably, electrodynamic and photothermal therapies mediated by EV-Pd-Pt nanoparticles exhibited synergistic benefits and great efficiency in elimination of S. aureus biofilms (Qiao et al, 2022).…”

Guanidine and Galactose Decorated Nanophotosensitizer with Oxygen Self‐Sufficient Capability for the Localized Ablation of Oral Biofilm

“…[ 21 ] However, the hypoxia in the infectious microenvironment greatly limits the therapeutic efficiency of the oxygen‐dependent PDT. [ 22 ] Copolymerized perfluoropolyether, an effective oxygen carrier, was utilized to build a self‐oxygenated system, for improving the efficiency of PDT and the oxidative degradation of the EPS skeleton in biofilms, thereby achieving the purpose of eradicating the oral biofilm. To reduce the toxicity of guanidine and interact with the carbohydrate‐binding site of bacteria, galactose was introduced to the nanophotosensitizer (Scheme S1, Supporting Information).…”