A Meta-Analysis in Assessing Oxidative Stress Using Malondialdehyde in Oral Submucous Fibrosis

Abstract

Objective This study aims to evaluate the oxidative stress marker Malondialdehyde (MDA) in oral submucous fibrosis with available literature. Materials and Methods We conducted a literature search electronically in PubMed (MeSH), Science Direct, Scopus, and Google Scholar using specific keywords. Results A systematic search in PubMed, Science Direct, and Google Scholar identified 334 articles. Of these, four were duplicate reports, and three were animal studies. After reading the abstracts of the collected articles, 288 articles were excluded for the following reasons: low quality, not relevant to the research question, or did not meet the inclusion criteria. The remaining 46 articles were chosen for full-text assessment. Finally, the present qualitative synthesis included 23 articles for evaluation. The selected studies in MDA analysis in a random-effects model showed higher heterogeneity (Q = 477.636, p < 0.001, I2 = 95.394%). The standard difference in mean MDA concentration between oral submucous fibrosis (OSMF) and healthy subjects was estimated as 2.73 nmol/mL (95% confidence interval: 2.08–3.38). Conclusion The selected studies showed significantly higher MDA levels in various biological samples of patients with OSMF. Therefore, further studies are needed to estimate oxidative stress levels by using different biomarkers in OSMF to direct future therapy.

Effects of oxidative stress regulation in inflammation-associated gastric cancer progression treated using traditional Chinese medicines: A review

“…MDA is a cytotoxic product formed by lipid peroxidation in the presence of oxygen free radicals which can react with proteins and nucleic acids, destroying cell membrane structure and function, changing membrane permeability, and participates in crosstalk with oxidative stress pathway target proteins to disrupt the redox balance and activate inflammatory factor and pro-oncogene expression, thus accelerating the progression of a variety of tumors (e.g., thyroid, lung, breast, and colon cancers). [8][9][10] It has been reported that oxidative stress damage is more severe with higher MDA content. [11] It has been found that H pylori (Hp) acts as a pro-inflammatory mediator, inducing DNA methylation, activating oxidative stress pathways, upregulating MDA content, and damaging the gastric mucosa leading to transformation of gastric "inflammation-carcinoma."…”

Distinct and Dissociable EEG Networks Are Associated With Recovery of Cognitive Function Following Anesthesia-Induced Unconsciousness

“…The effect size ranges from 0 to 100% and reflects the proportion of cross block covariance accounted for by each latent variable. It is calculated as the ratio of the square of its singular value to the sum of all squared singular values derived from the decomposition (Berman et al, 2014;Mišić et al, 2016). A design salience (optimal contrast) is depicted for each latent variable.…”

“…Bootstrap ratios were thresholded at the 99% confidence interval to generate a network of functional connectivities between EEG electrodes that reliably express the statistical effect (pattern of functional connectivity variance) captured by the latent variable. Positive bootstrap ratios indicate that the associated functional connectivity network expresses the contrast in the depicted orientation whereas negative bootstrap ratios indicate that the network expresses the contrast in the opposite orientation (Mišić et al, 2016).…”

Development and validation of a minimally invasive protocol for assessing oxidative stress markers in exfoliated oral cells

“…11,14,15 So far, only a few protocols have been proposed to assess oxidative stress markers in the oral mucosa. [16][17][18][19][20] Intracellular levels of ROS and GSH, as well as ΔΨm, are significant biomarkers of oxidative stress 7,21,22 that have been effectively employed to measure oxidative stress status in a variety of cells and can be semi-quantitatively measured using fluorescent probes. 8,10,11,16,[23][24][25] The use of exfoliated oral cells to assess oxidative stress biomarkers has several advantages and is a promising approach, as these cells can be easily collected, ensuring patient comfort and facilitating longitudinal studies with minimal discomfort.…”

“…8,10,11,16,[23][24][25] The use of exfoliated oral cells to assess oxidative stress biomarkers has several advantages and is a promising approach, as these cells can be easily collected, ensuring patient comfort and facilitating longitudinal studies with minimal discomfort. [16][17][18][19]21,22,26 Additionally, exfoliated oral cells constitute a dynamic and heterogeneous population that reflects the oral environment, offering a comprehensive understanding of the state of oxidative stress in the oral cavity. 21,22 However, to the best of our knowledge, there are no reports of standardization of protocols that allow the use of fluorescent probes to measure biomarkers of oxidative stress in oral cells.…”