A General, Label-Free and Homogeneous Electrochemical Strategy for Probing of Protease Activity and Screening of Inhibitor

Feng, Yongan; Liu, Gang; Zhang, Fan; Liu, Jianwen; La, Ming; Xia, Ning

doi:10.3390/mi13050803

Cited by 3 publications

(1 citation statement)

References 36 publications

(48 reference statements)

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“…Meanwhile, it was found that the Cu 2+ complexes performed with the His1-containing peptides reveal high activity for the catalytic oxidation of OPD to produce fluorescent 2,3-diaminophenazine (OPDox) and maintained excellent selectivity for PSA detection even in the presence of high concentration of interfering proteins [ 88 ]. Based on the difference in the redox potential and current of Cu 2+ before and after being coordinated by the His1- or His3-containing peptide, Feng et al proposed a general, label-free, and homogeneous electrochemical biosensor for the detection of protease activity [ 89 ]. The angiotensin-converting enzyme (ACE) and thrombin were tested as the analyte examples.…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Progress in the Development of Biosensors Based on Peptide–Copper Coordination Interaction

et al. 2022

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Copper ions, as the active centers of natural enzymes, play an important role in many physiological processes. Copper ion-based catalysts which mimic the activity of enzymes have been widely used in the field of industrial catalysis and sensing devices. As an important class of small biological molecules, peptides have the advantages of easy synthesis, excellent biocompatibility, low toxicity, and good water solubility. The peptide–copper complexes exhibit the characteristics of low molecular weight, high tenability, and unique catalytic and photophysical properties. Biosensors with peptide–copper complexes as the signal probes have promising application prospects in environmental monitoring and biomedical analysis and diagnosis. In this review, we discussed the design and application of fluorescent, colorimetric and electrochemical biosensors based on the peptide–copper coordination interaction.

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Section: Electrochemical Biosensorsmentioning

confidence: 99%

Progress in the Development of Biosensors Based on Peptide–Copper Coordination Interaction

et al. 2022

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Application of Oxygen Reduction Reaction, Oxygen Evolution Reaction and Hydrogen Evolution Reaction in Electrochemical Biosensing

Zhou¹

2022

International Journal of Electrochemical Science

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Signal amplification based on catalytic reactions is one of the effective strategies to improve the sensitivity of electrochemical biosensors in which additional chemicals as the substrates are acquired. Water and oxygen are two simple and natural small molecules existed in the reaction medium of electrochemical biosensors. In this work, we reviewed the progress in the design of electrochemical biosensors with water or oxygen as the reaction substrate by oxygen reduction reaction (ORR), oxygen evolution reaction (OER) or hydrogen evolution reaction (HER) for signal enhancement.

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Electrochemical Protein-based Bioanalytical Devices for Drug Analysis

Sanz

Diculescu

2023

CTMC

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Proteins are vital components of living cells and the loss of their native functions has been associated with a wide variety of medical conditions. From this point of view, investigation of the protein microenvironment is crucial to support the development of therapeutic approaches capable of ensuring cellular functions. Therefore, analytical assays for the detection, quantification, and characterization of proteins, drugs, and protein-drug complexes play an essential role in fundamental research and clinical applications. Electrochemistry arises as an alternative methodology for fast assessment of proteins and drugs and is attractive due to the adaptability to miniaturization and scalability of electroanalytical devices, which then can be further employed as strategies towards personalized medical care. Thus, this review summarizes electrochemical investigations in the past 10 years on protein-based analytical devices and biosensors. A general overview of electrochemical assays that integrate proteins with nanostructured materials and conductive polymers is presented. Applications of electrochemical assays and biosensors were divided into four categories. First, those designed for drug screening strategies that focus on targeting specific intracellular, extracellular, or membrane protein subdomains to modulate their functions, aggregation/misfolding of proteins, and protein degradation pathways. Then, drug metabolism assays that involve mimicking natural metabolic pathways to identify potential safety and efficacy issues related to a drug or its metabolites. The third was dedicated to electrochemical drug delivery systems with anchored drugs in the form of bioconjugates, while the fourth was dedicated to electroanalytical methodologies for quantitative drug assays, where the electroactivity of the target species is often used to correlate the electrochemical signal to their concentration.

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A General, Label-Free and Homogeneous Electrochemical Strategy for Probing of Protease Activity and Screening of Inhibitor

Cited by 3 publications

References 36 publications

Progress in the Development of Biosensors Based on Peptide–Copper Coordination Interaction

Progress in the Development of Biosensors Based on Peptide–Copper Coordination Interaction

Application of Oxygen Reduction Reaction, Oxygen Evolution Reaction and Hydrogen Evolution Reaction in Electrochemical Biosensing

Electrochemical Protein-based Bioanalytical Devices for Drug Analysis

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