A facile approach to obtain highly tough and stretchable LAPONITE®-based nanocomposite hydrogels

Liu, Xinyu; Niu, Xiaofeng; Fu, Zhinan; Liu, Liqun; Bai, Shengyu; Wang, Jie; Li, Li; Wang, Yiming; Guo, Xuhong

doi:10.1039/d0sm01132k

Cited by 30 publications

(26 citation statements)

References 29 publications

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“…[12,13] LAP clusters in polymers markedly affect the homogeneity of nanocomposites, resulting in a decrease in mechanical properties, while exfoliated and intercalated LAP can be homodispersed into polymers, leading to a cross-linking effect and therefore enhancing the mechanical properties. [31,32] Therefore, aqueous exfoliation of LAP is more commonly used for polymer functionalization. To address this issue, chemical modification is usually applied to completely exfoliate hydrophilic LAP in hydrophobic polymers, but it can cause premature degradation of polymers or can decrease the polymers' mechanical properties.…”

Section: Discussionmentioning

confidence: 99%

Nanosilicate‐Functionalized Polycaprolactone Orchestrates Osteogenesis and Osteoblast‐Induced Multicellular Interactions for Potential Endogenous Vascularized Bone Regeneration

Jin

Xiao

et al. 2021

Macromolecular Bioscience

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Massive oral and maxillofacial bone defect regeneration remains a major clinical challenge due to the absence of functionalized bone grafts with ideal mechanical and proregeneration properties. In the present study, Laponite (LAP), a synthetic nanosilicate, is incorporated into polycaprolactone (PCL) to develop a biomaterial for bone regeneration. It is explored whether LAP-embedded PCL would accelerate bone regeneration by orchestrating osteoblasts to directly and indirectly induce bone regeneration processes. The results confirmed the presence of LAP in PCL, and LAP is distributed in the exfoliated structure without aggregates. Incorporation of LAP in PCL slightly improved the compressive properties. LAP-embedded PCL is biocompatible and exerts pronounced enhancements in cell viability, osteogenic differentiation, and extracellular matrix formation of osteoblasts. Furthermore, osteoblasts cultured on LAP-embedded PCL facilitate angiogenesis of vessel endothelial cells and alleviate osteoclastogenesis of osteoclasts in a paracrine manner. The addition of LAP to the PCL endows favorable bone formation in vivo. Based upon these results, LAP-embedded PCL shows great potential as an ideal bone graft that exerts both space-maintaining and vascularized bone regeneration synergistic effects and can be envisioned for oral and maxillofacial bone defect regeneration.

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Section: Discussionmentioning

confidence: 99%

Nanosilicate‐Functionalized Polycaprolactone Orchestrates Osteogenesis and Osteoblast‐Induced Multicellular Interactions for Potential Endogenous Vascularized Bone Regeneration

Jin

Xiao

et al. 2021

Macromolecular Bioscience

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“…36,37 Recently, an increasing number of studies have shown the efficacy of LAP-containing materials in the osteogenesis of BMSCs and osteoblasts as well as their ability to promote bone regeneration. [38][39][40] Furthermore, LAP has been shown to degrade into non-toxic components (Na + , Mg 2+ , Si(OH) 4 , Li + ) in physiological conditions, which can promote cellular adhesion and stimulate osteogenesis. 41 Herein, we show the successful development of an RSF/LAP 3D porous scaffold by simply mixing LAP dispersion in a RSF aqueous solution, followed by the previously established procedure (n-butanol addition coupled with freezing treatment).…”

Section: Introductionmentioning

confidence: 99%

Protein–inorganic hybrid porous scaffolds for bone tissue engineering

Sun

Yao

et al. 2022

J. Mater. Chem. B

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“…LAP can act as a filler in hydrogels to promote mechanical properties and tune bone regeneration by regulating the stiffness of hydrogels [ 16–18 ]. Furthermore, its hydrophilic surface and negative charge can stimulate protein adsorption to increase cell adhesion and can be used as a therapeutic delivery vehicle to deliver a range of molecules [ 18 , 19 ]. An increasing number of studies have confirmed the bioactivity of LAP-containing biomaterials in the osteogenesis of BMSCs and osteoblasts and the promotion of bone regeneration in different bone defects [ 18 , 20–22 ].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, its hydrophilic surface and negative charge can stimulate protein adsorption to increase cell adhesion and can be used as a therapeutic delivery vehicle to deliver a range of molecules [ 18 , 19 ]. An increasing number of studies have confirmed the bioactivity of LAP-containing biomaterials in the osteogenesis of BMSCs and osteoblasts and the promotion of bone regeneration in different bone defects [ 18 , 20–22 ]. Most of them focused on incorporation of LAP into different polymers (such as PCL and PLGA) to obtain functionalized fibrous membranes via electrospinning for potential bone regeneration applications [ 22–26 ].…”

Section: Introductionmentioning

confidence: 99%

Vascularized bone regeneration accelerated by 3D-printed nanosilicate-functionalized polycaprolactone scaffold

Xiao

et al. 2021

Regenerative Biomaterials

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Critical oral-maxillofacial bone defects, damaged by trauma and tumors, not only affect the physiological functions and mental health of patients but are also highly challenging to reconstruct. Personalized biomaterials customized by 3D printing technology have the potential to match oral-maxillofacial bone repair and regeneration requirements. Laponite (LAP) nanosilicates have been added to biomaterials to achieve biofunctional modification owing to their excellent biocompatibility and bioactivity. Herein, porous nanosilicate-functionalized polycaprolactone (PCL/LAP) was fabricated by 3D printing technology, and its bioactivities in bone regeneration were investigated in vitro and in vivo. In vitro experiments demonstrated that PCL/LAP exhibited good cytocompatibility and enhanced the viability of bone marrow mesenchymal stem cells (BMSCs). PCL/LAP functioned to stimulate osteogenic differentiation of BMSCs at the mRNA and protein levels and elevated angiogenic gene expression and cytokine secretion. Moreover, BMSCs cultured on PCL/LAP promoted the angiogenesis potential of endothelial cells by angiogenic cytokine secretion. Then, PCL/LAP scaffolds were implanted into the calvarial defect model. Toxicological safety of PCL/LAP was confirmed, and significant enhancement of vascularized bone formation was observed. Taken together, 3D-printed PCL/LAP scaffolds with brilliant osteogenesis to enhance bone regeneration could be envisaged as an outstanding bone substitute for a promising change in oral-maxillofacial bone defect reconstruction.

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A facile approach to obtain highly tough and stretchable LAPONITE®-based nanocomposite hydrogels

Abstract: Laponite sheets have been widely used for the preparation of tough nanocomposite hydrogels for enticing applications, however, their inferior dispersion in aqueous media resulting from the electrostatic interactions between the...

Cited by 30 publications

References 29 publications

Nanosilicate‐Functionalized Polycaprolactone Orchestrates Osteogenesis and Osteoblast‐Induced Multicellular Interactions for Potential Endogenous Vascularized Bone Regeneration

Nanosilicate‐Functionalized Polycaprolactone Orchestrates Osteogenesis and Osteoblast‐Induced Multicellular Interactions for Potential Endogenous Vascularized Bone Regeneration

Protein–inorganic hybrid porous scaffolds for bone tissue engineering

Vascularized bone regeneration accelerated by 3D-printed nanosilicate-functionalized polycaprolactone scaffold

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