Protein–inorganic hybrid porous scaffolds for bone tissue engineering

Lu, Minqi; Sun, Liangyan; Yao, Jinrong; Zhao, Biwei; Liu, Yuehua; Shao, Zhengzhong; Chen, Xin

doi:10.1039/d2tb00853j

Cited by 8 publications

(8 citation statements)

References 54 publications

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“…The above results are consistent with the literature reports, that is, the incorporation of LAP into various polymer matrices stimulates osteogenic differentiation even in the absence of any osteoinductive factors [ 31 , 38 , 43 , 44 , 45 ]. For example, Xavier et al reported that after the addition of LAP, the ALP activity and calcium deposition of preosteoblast cells were significantly increased [ 44 ].…”

Section: Resultssupporting

confidence: 93%

“…Similarly, Miao et al also found that the protein expressions of Runx2 and Col1 were significantly higher with the addition of LAP [ 38 ]. Recently, a novel RSF/LAP 3D porous scaffold with good biocompatibility and osteogenesis capacity was successfully developed in this laboratory [ 43 ]. The ALP activity and osteogenic-related gene and protein expression levels (BMP-2, Runx2, and OPN) also increased gradually with the LAP content.…”

Section: Resultsmentioning

confidence: 99%

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Silk–Inorganic Nanoparticle Hybrid Hydrogel as an Injectable Bone Repairing Biomaterial

Sun

Chen

et al. 2023

JFB

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Silk fibroin is regarded as a promising biomaterial in various areas, including bone tissue regeneration. Herein, Laponite® (LAP), which can promote osteogenic differentiation, was introduced into regenerated silk fibroin (RSF) to prepare an RSF/LAP hybrid hydrogel. This thixotropic hydrogel is injectable during the operation process, which is favorable for repairing bone defects. Our previous work demonstrated that the RSF/LAP hydrogel greatly promoted the osteogenic differentiation of osteoblasts in vitro. In the present study, the RSF/LAP hydrogel was found to have excellent biocompatibility and significantly improved new bone formation in a standard rat calvarial defect model in vivo. Additionally, the underlying biological mechanism of the RSF/LAP hydrogel in promoting osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) was extensively explored. The results indicate that the RSF/LAP hydrogels provide suitable conditions for the adhesion and proliferation of BMSCs, showing good biocompatibility in vitro. With the increase in LAP content, the alkaline phosphatase (ALP) activity and mRNA and protein expression of the osteogenic markers of BMSCs improved significantly. Protein kinase B (AKT) pathway activation was found to be responsible for the inherent osteogenic properties of the RSF/LAP hybrid hydrogel. Therefore, the results shown in this study firmly suggest such an injectable RSF/LAP hydrogel with good biocompatibility (both in vitro and in vivo) would have good application prospects in the field of bone regeneration.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Silk–Inorganic Nanoparticle Hybrid Hydrogel as an Injectable Bone Repairing Biomaterial

Sun

Chen

et al. 2023

JFB

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“…The edges of newly formed bones ( Figure 10 c,f,i) displayed similar morphologies. The magnitude of the newly extended and formed bone was thought to be medium compared with other relevant studies [ 49 , 50 , 51 ].…”

Section: Discussionmentioning

confidence: 93%

Self-Prepared Hyaluronic Acid/Alkaline Gelatin Composite with Nano-Hydroxyapatite and Bone Morphogenetic Protein for Cranial Bone Formation

Hachinohe

Taira

Hoshi

et al. 2023

IJMS

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New bone-forming substitute materials are highly useful in dental implantology. The purpose of this study was to prepare cross-linked hyaluronic acid (cHLA)/cross-linked alkaline gelatin (cAG)/nano-hydroxyapatite (nHAp)/bone morphogenic protein (BMP) constructs; and evaluate their bone-forming capabilities in rat cranial bone defects. The cHLA and cAG liquids processed with an epoxy cross-linker were blended with a 3:1 volume ratio, followed by freeze-drying. The dry composites were further infiltrated with water containing nHAp only (BMP (−)) or with water containing nHAp and BMP (BMP (+)). Prepared wet constructs (BMP (−) and BMP (+)) were implanted in rat cranial bone defects, while defects only were also made, and animals were fed for 8 weeks, followed by subsequent soft X-ray measurements and histological observations. The X-ray results showed that BMP (+) constructs disappeared, though caused inward extension of peripherical bone from defect edges with an increase in length of approximately 24%, larger than those of BMP (−) constructs and defect only with approximately 17% and 8% increments, respectively (p < 0.05). Histological observations of BMP (+) construct samples clearly indicated active bone extension consisting of an array of island-like bones. It was concluded that cHLA/cAG/nHAp/BMP could be used as novel bone-substitute materials.

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“…Some novel approaches have been developed to create three-dimensional scaffolds based on biomaterials. Such scaffolds have advantages for tissue engineering, such as promoting cell adhesion, proliferation, migration, and differentiation [ 124 ]. Many natural and synthetic polymers produce scaffolds in skin tissue engineering [ 125 , 126 , 127 ].…”

Section: Biodegradable and Nondegradable Polymers For Wound Healingmentioning

confidence: 99%

Combinational System of Lipid-Based Nanocarriers and Biodegradable Polymers for Wound Healing: An Updated Review

Far

Naimi-Jamal

Sedaghat

et al. 2023

JFB

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Skin wounds have imposed serious socioeconomic burdens on healthcare providers and patients. There are just more than 25,000 burn injury-related deaths reported each year. Conventional treatments do not often allow the re-establishment of the function of affected regions and structures, resulting in dehydration and wound infections. Many nanocarriers, such as lipid-based systems or biobased and biodegradable polymers and their associated platforms, are favorable in wound healing due to their ability to promote cell adhesion and migration, thus improving wound healing and reducing scarring. Hence, many researchers have focused on developing new wound dressings based on such compounds with desirable effects. However, when applied in wound healing, some problems occur, such as the high cost of public health, novel treatments emphasizing reduced healthcare costs, and increasing quality of treatment outcomes. The integrated hybrid systems of lipid-based nanocarriers (LNCs) and polymer-based systems can be promising as the solution for the above problems in the wound healing process. Furthermore, novel drug delivery systems showed more effective release of therapeutic agents, suitable mimicking of the physiological environment, and improvement in the function of the single system. This review highlights recent advances in lipid-based systems and the role of lipid-based carriers and biodegradable polymers in wound healing.

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Protein–inorganic hybrid porous scaffolds for bone tissue engineering

Abstract: Porous scaffolds hold promise in the treatment of bone defects for bone tissue engineering due to their interconnected porous structure and suitable mechanical properties. Herein, Laponite (LAP), which is able...

Cited by 8 publications

References 54 publications

Silk–Inorganic Nanoparticle Hybrid Hydrogel as an Injectable Bone Repairing Biomaterial

Silk–Inorganic Nanoparticle Hybrid Hydrogel as an Injectable Bone Repairing Biomaterial

Self-Prepared Hyaluronic Acid/Alkaline Gelatin Composite with Nano-Hydroxyapatite and Bone Morphogenetic Protein for Cranial Bone Formation

Combinational System of Lipid-Based Nanocarriers and Biodegradable Polymers for Wound Healing: An Updated Review

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