A Drosophila male accessory gland protein that is a member of the serpin superfamily of proteinase inhibitors is transferred to females during mating

Coleman, Sean T.; DrÄhn, Brent; Petersen, Greg; Stolorov, Javor; Kraus, K W

doi:10.1016/0965-1748(94)00055-m

Cited by 64 publications

(34 citation statements)

References 15 publications

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“…These differences may result from low-level contamination by accessory gland fragments or cells in the large-scale testes preparations for the EST project or may indicate that Acp36DE is transcribed, but not translated, in the testes. Consistent with such models, all 15 Acp antibodies thus far generated detect exclusively accessory gland-specific proteins, even though 9 of 15 genes [CG8982 (Acp26Aa), CG4605 (Acp32CD), CG7157 (Acp36DE), CG6289, CG8137, CG9334, CG17575, CG1656, CG9029] (Monsma et al 1990;Coleman et al 1995;Bertram et al 1996;Lung et al 2002;Ravi Ram et al 2005) have testis EST hits (Andrews et al 2000;Rubin et al 2000a;Parisi et al 2003).…”

Section: Methodsmentioning

confidence: 86%

“…These 52 D. melanogaster ACPs are expected to be extracellular and thus transferred to the female upon mating and to be produced primarily or exclusively in the male's accessory gland. Indeed, all 16 Acp genes tested so far encode seminal proteins detectable only in the male's accessory gland and transferred to the female during mating (Chen et al 1988;Monsma et al 1990;Coleman et al 1995;Bertram et al 1996;Lung et al 2002;Albright 2003;Ravi Ram et al 2005). Additional support that this set of 52 D. melanogaster ACPs truly represents accessory-gland predominant genes stems from the finding that 29 of 46 tested Acp genes showed twofold or higher expression values in germlineless male vs. germlineless female comparisons (6 ACPs were not present on the microarrays) .…”

Section: Methodsmentioning

confidence: 99%

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Cross-Species Comparison of Drosophila Male Accessory Gland Protein Genes

et al. 2005

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Drosophila melanogaster males transfer seminal fluid proteins along with sperm during mating. Among these proteins, ACPs (Accessory gland proteins) from the male's accessory gland induce behavioral, physiological, and life span reduction in mated females and mediate sperm storage and utilization. A previous evolutionary EST screen in D. simulans identified partial cDNAs for 57 new candidate ACPs. Here we report the annotation and confirmation of the corresponding Acp genes in D. melanogaster. Of 57 new candidate Acp genes previously reported in D. melanogaster, 34 conform to our more stringent criteria for encoding putative male accessory gland extracellular proteins, thus bringing the total number of ACPs identified to 52 (34 plus 18 previously identified). This comprehensive set of Acp genes allows us to dissect the patterns of evolutionary change in a suite of proteins from a single male-specific reproductive tissue. We used sequence-based analysis to examine codon bias, gene duplications, and levels of divergence

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Section: Methodsmentioning

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Cross-Species Comparison of Drosophila Male Accessory Gland Protein Genes

et al. 2005

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“…In A. mellifera and Bombus terrestris L., these glands seem to be involved in the production of a mucous plug that prevents the reflux of sperm after copulation (Bishop 1920, Snodgrass 1956). Since mating has an effect on vitellogenesis, or on the maturation of oocytes in the ovary of insects (Chen 1984, Coleman et al 1995, it is possible that these glands may produce substances that regulate fertility and the sexual behavior of the females (Dallacqua & Cruz-Landim 2003). According to Colonello & Hartfelder (2003), the protein content increases in males of A. mellifera from newly emerged immature drones to sixday old drone.…”

Section: Resultsmentioning

confidence: 99%

Comparative anatomy of the male reproductive internal organs of 51 species of bees

Ferreira¹,

Abdalla²,

Kerr³

et al. 2004

Neotrop. Entomol.

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Anatomia Comparada dos Órgãos do Aparelho Reprodutor Masculino de 51 Espécies de AbelhasRESUMO -A anatomia dos órgãos internos do aparelho reprodutor de machos (ARM) adultos e pupas foi comparada em 51 espécies de abelhas, incluindo representantes de seis famílias. Foram obtidos quatro tipos diferentes de ARM. O tipo I está presente em machos das famílias mais basais (Colletidae, Andrenidae e Halictidae) e é caracterizado por três túbulos seminíferos por testículo, o qual é quase totalmente envolvido pela membrana escrotal. O tipo II é um tipo intermediário entre os tipos I e III e está presente em Mellitidae e Megachilidae, como também em alguns Apidae estudados, sendo caracterizado por possuir dutos deferentes pós-vesiculares fora da membrana escrotal e possuir três ou quatro túbulos seminíferos por testículo, exceto Apis mellifera L., a qual possui secundariamente um número aumentado de túbulos. O tipo III foi achado somente nos Apidae estudados e é caracterizado por apresentar os testículos e dutos genitais (exceto o duto deferente pós-vesicular) encapsulados separadamente, as glândulas acessórias são bem desenvolvidas e o duto ejaculador é calibroso, apresentando fissuras em sua parede externa, as quais podem ocorrer também no tipo II. O tipo IV está presente exclusivamente na tribo Meliponini e é caracterizado pela ausência de glândulas acessórias. PALAVRAS-CHAVE: Glândula acessória, genitália, morfologiaABSTRACT -The anatomy of the internal organs of the male reproductive apparatus (MRA) of adults and pupae was compared among 51 species of bees, including representatives of six families. Four different types of MRA were found. The type I is present in males of the less derived families (Colletidae, Andrenidae, and Halictidae) and is characterized by three seminiferous tubules per testis, which are almost completely enveloped by the scrotal membrane. The type II is an intermediary between types III and I and is present in Melittidae and Megachilidae, as well as in some Apidae studied, being characterized by post-vesicular deferent ducts outside the scrotal membrane and by three or four seminiferous tubules per testis, except for Apis mellifera L., which has a secondarily increased number of tubules. Type III was only found in the Apidae studied and is characterized by separately encapsulated testes and genital ducts (except for the post-vesicular deferent duct). Accessory glands are well developed, and the ejaculatory duct is thickened with fissures in its wall, which may also occur in the type II. Type IV is present exclusively in the tribe Meliponini, and is characterized by the absence of accessory glands.

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“…Therefore the mating is a stimulating factor to start vitellogenesis and oocyte maturation. Several results obtained mainly studying Drosophila (Schmidt et al, 1993;Coleman et al, 1995;Wolfner, 1997;Tram & Wolfner, 1998) demonstrate that the mating affect the ovarian development, the egg laying rates, and even the female longevity. These effects are attributed to substances introduced into the genital tract of the female with the sperm, produced by the accessory glands of the male reproductive apparatus.…”

Section: Discussionmentioning

confidence: 99%

Mating influence in the ovary differentiation in adult queens of Apis mellifera L. (Hymenoptera, Apidae)

Patrício¹,

Cruz-Landim²

2002

Braz. J. Biol.

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The present results show that in the ovarioles of a newly emerged (0 day) queen of A. mellifera only two regions may be distinguished: a proximal, short germarium and a very long distal, terminal filament. As the queen matures and gets ready for the nupcial flight, the germarium increases in lenght, advancing towered the distal end, as the terminal filament shortens. The ovarioles of queens ready to mate (6 to 8 days old) have, already one or two ovarian follicles, i.e. a very short proximal vitellarium, but a real vitellogenesis only starts after the fecundation. If the queen does not mate the ovarioles structure is disrupted (12-16 days old). In mated queen eggs the ovarioles present three differentiated regions, from the apice to the basis: a short terminal filament, a medium size germarium, and a very long basal vitellarium. As the eggs are laid, the emptied follicle collapses, degenerates and produces a corpus luteum.Key words: mating, germarium, vitellarium, oocyte. RESUMOInfluência do acasalamento na diferenciação do ovário em rainha adulta de Apis mellifera L. (Hymenoptera, Apidae) Os resultados do presente trabalho demonstram que nos ovaríolos da rainha de Apis mellifera recém-emergida (0 dia) apenas duas regiões podem ser distinguidas: um curto germário proximal e um longo filamento terminal distal. À medida que a rainha amadurece e fica pronta para o vôo nupcial, o comprimento do germário aumenta e avança em direção à região distal e o filamento terminal diminui. Os ovaríolos da rainha pronta para acasalar (6 a 8 dias) têm 1 ou 2 folículos ovarianos, isto é, um vitelário proximal muito pequeno, mas a vitelogênese propriamente dita somente se inicia após a fecundação. Nas rainhas não acasaladas os ovaríolos se desestruturam (12-16 dias). Nas rainhas acasaladas os ovários apresentam os ovaríolos divididos em três regiões, do ápice para a base: um filamento terminal pequeno, um germário médio e um vitelário muito longo. Quando os ovos são postos, os folículos esvaziados colapsam, produzem um corpo lúteo e finalmente degeneram.Palavras-chave: acasalamento, germário, vitelário, ovócito.

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A Drosophila male accessory gland protein that is a member of the serpin superfamily of proteinase inhibitors is transferred to females during mating

Cited by 64 publications

References 15 publications

Cross-Species Comparison of Drosophila Male Accessory Gland Protein Genes

Cross-Species Comparison of Drosophila Male Accessory Gland Protein Genes

Comparative anatomy of the male reproductive internal organs of 51 species of bees

Mating influence in the ovary differentiation in adult queens of Apis mellifera L. (Hymenoptera, Apidae)

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