A confirmatory test for sperm in sexual assault samples using a microfluidic-integrated cell phone imaging system

Deshmukh, Shreya; İnci, Fatih; Karaaslan, Merve Gökşin; Ogut, Mehmet Giray; Duncan, Derek; Klevan, Leonard; Duncan, George; Demirci, Utkan

doi:10.1016/j.fsigen.2020.102313

Cited by 15 publications

(9 citation statements)

References 34 publications

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“…The test result is usually not provided to the patient until the next day with such methods ( Figure 1 ). As a result, decentralized testing as a point-of-care (PoC) concept is critical for the timely and accurate detection of such diseases [ 38 , 39 , 40 ] and any conditions that require further downstream studies [ 41 , 42 , 43 , 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

Biosensors as Nano-Analytical Tools for COVID-19 Detection

Pradhan

Lahare

Sinha

et al. 2021

Sensors

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Selective, sensitive and affordable techniques to detect disease and underlying health issues have been developed recently. Biosensors as nanoanalytical tools have taken a front seat in this context. Nanotechnology-enabled progress in the health sector has aided in disease and pandemic management at a very early stage efficiently. This report reflects the state-of-the-art of nanobiosensor-based virus detection technology in terms of their detection methods, targets, limits of detection, range, sensitivity, assay time, etc. The article effectively summarizes the challenges with traditional technologies and newly emerging biosensors, including the nanotechnology-based detection kit for COVID-19; optically enhanced technology; and electrochemical, smart and wearable enabled nanobiosensors. The less explored but crucial piezoelectric nanobiosensor and the reverse transcription-loop mediated isothermal amplification (RT-LAMP)-based biosensor are also discussed here. The article could be of significance to researchers and doctors dedicated to developing potent, versatile biosensors for the rapid identification of COVID-19. This kind of report is needed for selecting suitable treatments and to avert epidemics.

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Section: Discussionmentioning

confidence: 99%

Biosensors as Nano-Analytical Tools for COVID-19 Detection

Pradhan

Lahare

Sinha

et al. 2021

Sensors

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“…[301][302][303][304] Compared with conventional macroscale vessels, microfluidic devices offer several advantages, including i) low cost and large-scale production with desired parameters, ii) portability, iii) low reagent consumption, iv) short assay time, v) easy processing of samples, vi) the development of multiple control units with the modulation of surface properties, and vii) high mixing efficiency. [305][306][307][308][309][310][311] Furthermore, microfluidic devices provide a high surface-tovolume ratio, leading to high mass and heat transfer rates. [312] To date, microfluidic devices have several applications in various research fields, including protein engineering, [313] immunoassays, [314] DNA research.…”

Section: An Integrative Platform: Microfluidicsmentioning

confidence: 99%

Carbon‐Based Nanomaterials and Sensing Tools for Wearable Health Monitoring Devices

Erdem

Derin

Shirejini

et al. 2021

Adv Materials Technologies

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In this manner, sensor technologies have garnered great attention in various fields, including biomedicine, [2][3][4][5] environmental monitoring, [6][7][8][9] smart devices, [10] wearable devices, [11] automobile manufacturing [12] since the semiconductor materials and circuits have been developed. In particular, biosensors are powerful and innovative analytical tools that incorporate biological receptors to recognize biological analytes through either physical or chemical transducers. Primarily, bio-receptors are responsible for identifying and capturing target analytes, and the transducer basically translates biological and chemical information into the detectable signals, which are eventually converted into the concentration of the analyte. [13,14] Considering gold standard methods, such as enzyme-linked immunosorbent assay (ELISA) [15] and polymerase chain reaction (PCR)-based strategies, [16] biosensors mostly hold crucial features, such as i) short assay time, [17] ii) affordable tools and reagents, [18] iii) portability, [19] and iv) facile use and minimum user interpretation. [20] Nowadays, the applications of biosensors have been leveraged by the advancements of portable and miniaturized platforms. In particular, over the past years, wearable health monitoring devices have notable impact on continuous and real-time monitoring of health parameters, thereby accelerating the deployment of biosensing strategies to daily lives. Besides, non-invasive and ease-of-collecting information supports the benefits of the wearable systems for enhancing the awareness of individuals and communities. [21][22][23] The special features of the mechanically flexible and stable wearable sensors include remarkable means, such as portability, comfortability, light-weight, non-invasive, and reliable performance. To put it simply, wearable sensors are readily attached to skin or organ surfaces through an adhesive tape [24] or microneedles, [25] and because of such easy integrations, several researchers have focused on developing wearable sensors for real-time health monitoring. A wearable sensor is basically composed of some vital elements, including a flexible base material attached to the skin or an organ, a signal transfer electrode, and a biorecognition element. Recently, researchers have concentrated on creating integrated sensors that are able to measure various parameters simultaneously, such as pressure, temperature,The healthcare system has a drastic paradigm shift from centralized care to home-based and self-monitoring strategies; aiming to reach more individuals, minimize workload in hospitals, and reduce healthcare-associated expenses. Particularly, wearable technologies are garnering considerable interest by tracking physiological parameters through motion and activities, and monitoring biochemical markers from sweat, saliva, and tears. Through their integrations with sensors, microfluidics, and wireless communication systems, they allow physicians, family members, or individuals to monitor multiple parameters withou...

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“…Enrichment reliant on magnetic affinity is another method, in which both immunomagnetic assays and microfluidics are employed to distinguish rare cells and cellular entities. [119][120][121][122] As previously mentioned, the CellSearch platform is the first validated CTCs enrichment assay using magnetic fields. [27,99] On the other hand, immunomagnetic cell separation and density gradient are some of the first recorded studies of CTM isolation from whole human blood.…”

Section: Magnetic Affinity-based Selectionmentioning

confidence: 99%

Smart Material‐Integrated Systems for Isolation and Profiling of Rare Cancer Cells and Emboli

Sagdic

İnci

2021

Adv Eng Mater

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This review presents a broad aspect of smart material‐integrated systems for isolating and profiling rare circulating tumor cells (CTCs) and circulating tumor microemboli (CTM) to provide physiological, biological, and mechanistic insights into cancer research. In particular, CTCs/CTM have emanated as essential pieces of evidence that can reveal clonal evolution, tumor heterogeneity, and disease progression within the metastatic cascade. Morphologies, cellular compositions, and rarity of CTCs/CTM make them difficult to track and isolate for profiling distinct molecular characteristics in the case of metastatic potential. Accordingly, with the advanced‐characterization techniques, examining the aspects of the specific surface markers of CTCs/CTM, epithelial‐to‐mesenchymal (EMT), mesenchymal‐to‐epithelial (MET) transitions, and timing of tumor cell dissemination would assist us to understand cancer biology and metastatic characteristics. Existing clinical and research methods for the enrichment and isolation of these sporadic cells depend on mainly conventional methods with low‐yield and expensive features. Owing to their specialized functions and analytical performances, smart material‐based technologies hold an enormous impact not only on cell detection, but also on cell isolation for downstream analyses. Herein, the main reasons for cell isolation are discussed and the recent developments in CTCs/CTM approaches for identifying further methods and future perspectives are elaborated on.

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A confirmatory test for sperm in sexual assault samples using a microfluidic-integrated cell phone imaging system

Cited by 15 publications

References 34 publications

Biosensors as Nano-Analytical Tools for COVID-19 Detection

Biosensors as Nano-Analytical Tools for COVID-19 Detection

Carbon‐Based Nanomaterials and Sensing Tools for Wearable Health Monitoring Devices

Smart Material‐Integrated Systems for Isolation and Profiling of Rare Cancer Cells and Emboli

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