A comparison of two techniques for the determination of serum bone-specific alkaline phosphatase activity in dogs

Allen, Lynn C.; Allen, Matthew J.; Breur, Gert J.; Hoffmann, Walter; Richardson, Daniel C.

doi:10.1053/rvsc.1999.0369

Cited by 39 publications

(37 citation statements)

References 29 publications

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“…In this study, when analyzing the biological variability of these two biomarkers, the serum activity of tALP and BALP presented a lower value of within-subject variability than serum TRAP activity and this last enzyme showed the lowest inter-individual variation, a fact also observed by Panteghini and Pagani (1995) in man. Moreover, the high rate of interindividual variation found for the serum tALP activity was similar to previously data published for the dog (Allen et al, 2000;Kaneko et al, 2008). Additionally, the intra-and interindividual variations found for TRAP was similar to that found for other resorption biomarkers for this species such as ICTP in the study of Ladlow et al (2002), and similar to that found in humans in the study of Panteghini and Pagani (1995).…”

Section: Discussionsupporting

confidence: 91%

Tartrate-resistant acid phosphatase as a biomarker of bone turnover in dog

Sousa¹,

Nery²,

Azevedo³

et al. 2011

Arq. Bras. Med. Vet. Zootec.

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Values of serum tartrate-resistant acid phosphatase (TRAP) activity were obtained in adult dogs and its biological variability was assessed. Nine healthy skeletally mature Portuguese Podengo dogs were used for the determination of TRAP, total and bone alkaline phosphatase serum activities, and also to study their relationship with serum minerals, namely calcium (Ca), phosphorous (P), and magnesium (Mg). The serum TRAP activity was 2.19±0.56IU/mL, with intra-individual variation of 18.3% and inter-individual variation of 25.6%. Significant correlations were observed between serum TRAP activity and Ca (r=-0.3431; P<0.05), Ca and Mg (r=-0.787; P<0.01), and TRAP and Mg (r=0.397; P<0.05). The results indicate that serum TRAP activity in dog could be of great value in research and in clinical practice, providing complementary non-invasive information on bone metabolism.Keywords: dog, bone metabolism, bone resorption, osteoclast de 2,19±0,56 UI/mL,3% e interindividual de 25,6%. Foram observadas correlações significativas ao longo do tempo entre 3431; P<0,05), entre FART e magnésio (r=0,3974; P<0,05) e entre cálcio e magnésio (r=-0,787; P<0,01). Os resultados indicam que este marcador de reabsorção óssea pode ser de grande valor na prática clínica e na investigação e, ainda, ser utilizado como um método auxiliar não invasivo para avaliação do metabolismo ósseo. Palavras-chave: cão, metabolismo ósseo, reabsorção, osteoclaste RESUMO Determinaram-se os valores da atividade da fosfatase ácida resistente ao tartarato (FART) e avaliou-se a sua variabilidade biológica. Neste estudo, foram utilizados nove cães adultos e saudáveis de raça Podengo Português para as determinações das atividades da FART, da fosfatase alcalina total, da isoenzima óssea da fosfatase alcalina e da concentração dos minerais séricos -cálcio, fósforo e magnésio. A atividade sérica obtida da FART foi

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Section: Discussionsupporting

confidence: 91%

Tartrate-resistant acid phosphatase as a biomarker of bone turnover in dog

Sousa¹,

Nery²,

Azevedo³

et al. 2011

Arq. Bras. Med. Vet. Zootec.

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“…A previous study reported a poor (r ϭ .20) correlation between TALP and BALP activities in normal adult Beagle dogs. 26 On the basis of the results, measurement of TALP activity as part of a standard pretreatment biochemical profile is adequate for routine clinical prognostication for dogs with osteosarcoma. For dogs with hyperadrenocorticism or biliary stasis, or for dogs receiving exogenous corticosteroids, BALP activity may more accurately reflect prognosis.…”

Section: Discussionmentioning

confidence: 99%

“…Reference ranges for alkaline phosphatase isoenzyme activity are known to vary between laboratories and with the age of the patient. The age group-based reference ranges for each isoenzyme used in the present study are based on a small group of dogs 26 and may not accurately represent true reference ranges. On the basis of these ranges, only 4 of 61 dogs were considered to have increased pretreatment LALP activity, jvim 14 614 Mp 591 File # 14em limiting the power of the log rank test (1 Ϫ ␤ ϭ 0.25, or 25% power) to detect a difference between groups.…”

Section: Discussionmentioning

confidence: 99%

Prognostic Significance of Serum Alkaline Phosphatase Activity in Canine Appendicular Osteosarcoma

Garzotto

Berg

Hoffmann

et al. 2000

Veterinary Internal Medicne

101

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Sixty-one dogs with appendicular osteosarcoma were treated with amputation and chemotherapy of cisplatin and doxorubicin. Serum samples were obtained before and after treatment for determination of total alkaline phosphatase (TALP) activity as well as the activities of the constituent bone (BALP), liver (LALP), and corticosteroid-induced (CALP) isoenzymes. The relationship between alkaline phosphatase activities and survival was examined by Cox proportional hazards regression analysis and KaplanMeier log rank analysis. Mean activity of TALP, BALP, and LALP decreased significantly after treatment (P Ͻ .001). TALP and LALP activities before treatment were significantly correlated with survival (P ϭ .006 and .001, respectively). The correlation between BALP activity before treatment and survival approached significance (P ϭ .054). CALP activity and TALP, BALP, and LALP activities after treatment were not significantly correlated with survival. Dogs with normal pretreatment TALP and BALP activities survived significantly longer than dogs with increased pretreatment activities (P ϭ .001 and .003, respectively). Median survival times for dogs with normal or increased TALP activities before treatment were 12.5 and 5.5 months, respectively; and median survival times for dogs with normal or increased BALP activities before treatment were 16.6 and 9.5 months, respectively. In the design of future clinical trials involving dogs with osteosarcoma, consideration should be given to stratifying the randomization according to alkaline phosphatase activity. In addition, alkaline phosphatase activity should be a factor considered by clinicians attempting to tailor the aggressiveness of adjuvant chemotherapy to the needs of individual patients or owners.

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“…Neste estudo, não se observou correlação significativa negativa ou positiva entre a OC e o ICTP. Porém, era esperado que houvesse correlação entre os marcadores de formação e reabsorção óssea, fato já verificado em gatos (DELAURIER et al, 2004), em cães (ALLEN et al, 2000) e em equinos (PRICE et al, 1995). Segundo DELAURIER et al (2004), a correlação indica que, em gatos sadios com o esqueleto maturo, os processos de formação e reabsorção óssea poderão estar intimamente associados.…”

Section: Discussionunclassified

“…Em relação à radiografia simples, a DMO, além de detectar a osteopenia, possui a vantagem de melhorar a identificação e a quantificação das alterações regionais ou sistêmicas (ALLEN et al, 2000), porém poucos centros veterinários utilizam esse meio diagnóstico na prática clínica. Um dos inconvenientes da densitometria óssea é a incapacidade de distinguir se a alteração foi provocada por efeitos da formação ou da reabsorção óssea.…”

Section: A Formação E Reabsorção óSsea São Processos Contínuos No Orgunclassified