A comparison of four different microleakage tests for assessment of leakage of root canal fillings

Karagenc, B.; Gençoğlu, Nimet; Ersoy, Mustafa; Cansever, Galip; Külekçi, Güven

doi:10.1016/j.tripleo.2005.10.044

Cited by 50 publications

(42 citation statements)

References 19 publications

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“…However, results vary depending on the method employed, creating serious concerns for reproducibility, and suggesting that a more critical analysis of the available models is needed. 7,8,11,16 Among the methods currently employed to determine leakage, the one described by Xu et al 13 has been preferred, because it uses glucose as a tracer, and allows continuous observer-independent measurements of a high number of specimens. However, the system (UV-VIS) detection efficiency was not adequate for making microleakage measurements, and some interfaces of the apparatus were sealed with cyanoacrylate, a material that has been widely criticized for failing to prevent leakage.…”

Section: Discussionmentioning

confidence: 99%

“…1 When new sealing materials or techniques emerge, studies should be performed to warrant their clinical application and to accurately assess their characteristics and properties, particularly their sealing ability. Several methods aimed at detecting leakage have already been described in the literature, such as the conductometric technique, 2 fluid filtration, 3,4 radioisotopes, 5 bacterial penetration, 6 the electrochemical test, 7 and the use of dyes. 8,9 However, all these methods have yielded controversial results, and have therefore been widely criticized.…”

Section: Introductionmentioning

confidence: 99%

“…8,9 However, all these methods have yielded controversial results, and have therefore been widely criticized. 7,8,10,11 Some studies have presented improvements to leakage assessment methods. Chittoni et al 12 used back-scattered electron imaging to better delimit visualization of gaps in retrofilling materials, using a scanning electron microscope (SEM).…”

Section: Introductionmentioning

confidence: 99%

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Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage

et al. 2015

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The objective of this study was to describe a new method for the quantitative analysis of a microleakage of endodontic filling materials. Forty extracted single-rooted teeth were randomly divided into three experimental groups. After root canal shaping, the experimental groups were filled using the lateral condensation technique with the Epiphany system (G1), with gutta-percha + Sealapex (G2), and with gutta-percha + AH Plus (G3). Each root was mounted on a modified leakage testing device, and caffeine solution was used as a tracer (2000 ng mL -1 , pH 6.0), applied in the coronal direction towards the tooth apex, creating a hydrostatic pressure of 2.55 kPa. Presence of caffeine in the receiving solution was measured after 10, 30, and 60 days, using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). None of the groups presented microleakage at 10 days. At 30 days, G2 and G3 showed similar infiltration patterns (means: 16.0 and 13.9 ng mL -1 , respectively), whereas G1 showed significantly higher values (mean: 105.2 ng mL -1 ). At 60 days, leakage values were 182.6 ng mL -1 for G1, 139.0 ng mL -1 for G2, and 53.5 ng mL -1 for G3. AH Plus showed the best sealing ability and HPLC-MS/MS showed high sensitivity and specificity for tracer quantification.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage

et al. 2015

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“…According to Karagenç et al,14 the difference in results obtained when using various methods to assess leakage may be attributed to the differences in the working principles of various tests methods and the different nature of obturation materials.…”

Section: Discussionmentioning

confidence: 99%

“…Although thermoplastic techniques show a certain superiority in achieving gutta-percha density, compared to cold techniques, 5 neither technique can effectively prevent the leakage, which has been analyzed by several different methods. [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] Considering that different filling techniques and sealers have resulted in similar apical seals, [20][21][22] it seems wise to investigate other reasons associated with greater or lesser leakage rates.…”

Section: Introductionmentioning

confidence: 99%

Lack of correlation between obturation limits and apical leakage

et al. 2013

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The aim of this paper was to evaluate a possible correlation between obturation limits and leakage. Thirty-six extracted human mandibular incisors were used, characterized by straight and single canals, non-anatomical complexities, absence of previous endodontic treatment, complete root formation and patent foramen. For standardization of the specimens for the leakage analysis, foraminal instrumentation was performed up to a Flexofile #25 (Dentsply-Maillefer, Ballaigues, Switzerland). All specimens were instrumented and filled following the same protocol, and the obturation limits were measured using Axiovision 4.5 Software (Carl Zeiss Vision, Hallbergmoos, Germany). The specimens were then separated into three groups (n = 12) according to the following variables: Group I -obturation limits ranging from 0 mm to 0.76 mm of the main apical foramen. Group II -obturation limits ranging from 0.77 mm to 0.98 mm of the main apical foramen. Group III -obturation limits ranging from 0.99 mm to 1.68 mm of the main apical foramen. Apical leakage was quantified by fluid filtration. The analyses were confronted using Pearson's test (p > 0.05). Groups I, II and III showed Pearson correlation values (r 2 ) of −0.152, −0.186 and 0.058, respectively. No correlation was found between the obturation limits and apical leakage.

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Innovative root-end filling materials based on calcium-silicates and calcium-phosphates

Abedi-Amin

Luzi

Giovarruscio

et al. 2017

J Mater Sci: Mater Med

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This in vitro study evaluated the apical sealing ability, bioactivity and biocompatibility of an experimental calcium silicate-based and two light-curing calcium silicate/calcium-phosphate cements as potential root end filling materials. A calcium silicate Portland-based (Control PC), an experimental calcium silicate (Exp. PC) and two light-curing cements (LC-CaP; LC-Si/CaP) were assessed for their alkalinising activity (pH) and biocompatibility. Single-rooted human canines were endodontically treated, filled with gutta-percha and finally submitted to apicoectomy. Root end fillings were performed using all tested cements, and their apical sealing ability was evaluated up to 4 weeks of immersion in simulated body fluid (SBF). The mineral precipitation at the apical region and the cement adaptation to root dentine were also evaluated through non-destructive optical microscopy both at 24 h and after prolonged water storage (four week). LC-CaP and LC-Si/CaP had neutral pH, the greatest sealing ability (24 h) and excellent cytocompatibility. The Exp. PC cement presented sealing ability after two and four weeks, as well as biocompatibility after four and seven days, similar to LC-CaP and LC-Si/CaP. The control PC cement showed the lowest sealing ability and the greatest cytotoxicity. Mineral precipitation was observed in all groups, while some differences were seen in terms of cement adaptation along the root canal dentine walls. The experimental light-curable cements as well as the experimental PC might be suitable root end filling materials with appropriate (in vitro) sealing ability, biocompatibility and aptitude to induce mineral precipitation.

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A comparison of four different microleakage tests for assessment of leakage of root canal fillings

Cited by 50 publications

References 19 publications

Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage

Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage

Lack of correlation between obturation limits and apical leakage

Innovative root-end filling materials based on calcium-silicates and calcium-phosphates

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