A comparative study on analytical method of total alkaloids from cortex Phellodendri amurens by reversed phase high performance liquid chromatography (RP-HPLC) and pressurized capillary electrochromatography (pCEC)
Abstract:Abstract:A pressurized capillary electrochromatography (pCEC) method with post-column detection cell has been developed for the analysis of total alkaloids of cortex Phellodendron amurense Rupr., Rutaceae. The separation of total alkaloids (berberine, palmatine, oatrorrhizine, magnoflorine, phellodendrine, candicine, menisperine) was optimized by compositions of the mobile phase, ionic strength of buffers, pH value, and applied voltage. Separation of total alkaloids was achieved within 11 min by using a mobile… Show more
“…Alkaloids are the major active compounds in HB and GHB. 6,23,24 Additionally, among them, berberine is known to have anti-inammatory, hepatoprotective, anti-oxidant effects. 25 Besides berberine, the main protoberberine-type alkaloids also exhibited various biological activities similar with that of berberine.…”
Non-targeted metabolite profiling and specific targeted discrimination strategy coupled with pattern recognition to differentiate the two varieties in rats.
“…Alkaloids are the major active compounds in HB and GHB. 6,23,24 Additionally, among them, berberine is known to have anti-inammatory, hepatoprotective, anti-oxidant effects. 25 Besides berberine, the main protoberberine-type alkaloids also exhibited various biological activities similar with that of berberine.…”
Non-targeted metabolite profiling and specific targeted discrimination strategy coupled with pattern recognition to differentiate the two varieties in rats.
CE and CEC, due to their versatility and high efficiency, have attracted great interest in the analysis of phytochemicals in herbs and their preparations. Previously, we reviewed the analysis of phytochemical bioactive compounds by CE in 2006 (Electrophoresis 2006, 27, 4808-4819) or CEC in 2010 (Electrophoresis 2010, 31, 260-277). This review followed the previous studies and covered the literature published since 2006 for CE and 2009 for CEC (excluding those mentioned in the two previous reviews), which emphasized the development of CE and CEC techniques in phytochemical analysis. In addition, sample preparation and detection were also discussed.
Development of the identification method of alkaloid compounds in Amur cork tree as well as not examined so far Oregon grape and European Barberry shrubs are presented. The novel approach to separation of alkaloids was applied and the capillary-high-performance liquid chromatography (capillary-HPLC) system was used, which has never previously been reported for alkaloid-based dyestuffs analysis. Its optimization was conducted with three different stationary phases (unmodified octadecylsilane-bonded silica, octadecylsilane modified with polar groups and silica-bonded pentaflourophenyls) as well as with different solvent buffers. Detection of the isolated compounds was carried out using diode-array detector (DAD) and tandem mass spectrometer with electrospray ionization (ESI MS/MS). The working parameters of ESI were optimized, whereas the multiple reactions monitoring (MRM) parameters of MS/MS detection were chosen based on the product ion spectra of the quasi-molecular ions. Calibration curve of berberine has been estimated (y = 1712091x + 4785.03 with the correlation coefficient 0.9999). Limit of detection and limit of quantification were calculated to be 3.2 and 9.7 ng/mL, respectively. Numerous alkaloids (i.e., berberine, jatrorrhizine and magnoflorine, as well as phellodendrine, menisperine and berbamine) were identified in the extracts from alkaloid plants and silk and wool fibers dyed with these dyestuffs, among them their markers.
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