Capillary‐HPLC with tandem mass spectrometry in analysis of alkaloid dyestuffs – a new approach

Jiang

J of Separation Science

et al. 2019

Berberine alkaloids, a group of protoberberine alkaloids under the classification of isoquinoline alkaloids, include berberine, coptisine, palmatine, columbamine, dehydrocorydaline, jatrorrhizine, and epiberberine from natural sources. Studies have shown that berberine alkaloids have various pharmacological functions, such as antibacterial, antiviral, blood pressure‐lowering, hypoglycaemic, antiarrhythmia, and anticancer effects. Therefore, it is worthwhile to develop analytical methods to investigate the pharmacokinetics and activity mechanisms of berberine alkaloids and to study berberine alkaloids more comprehensively. Current analytical methods for berberine alkaloids include liquid chromatography, thin‐layer chromatography, ultraviolet spectroscopy, capillary electrophoresis, and gas chromatography. The most widely used detection method is mass spectrometry. In order to provide a systematic and comprehensive summary and to serve as a reference for the future pharmacokinetics studies and analysis of berberine alkaloids, analytical methods for natural berberine alkaloids that have been used in the past ten years are reviewed here.

Section: Methodsmentioning

confidence: 99%

Section: Cementioning

confidence: 99%

A review on analytical methods for natural berberine alkaloids

Jiang

J of Separation Science

et al. 2019

“…; Dąbrowski et al . ). At 3.03 min, m/z 342.1726 with product ions m/z 192.0962 and 177.0737 was identified as another alkaloid, named phellodendrine.…”

Section: Resultsmentioning

confidence: 97%

“…Barberry usually refers to Berberis vulgaris L., with a low dyeing effectiveness, which is generally distributed over the greater part of Europe (Dąbrowski et al . ). Hence, the two plants should not be used to dye the yellow tassels.…”

Section: Resultsmentioning

confidence: 97%

Characterizations of palace lantern tassels preserved in The Palace Museum, Beijing, by UPLC‐ESI‐Q‐TOF

et al. 2020

Palace lanterns were important and highly visible decorations in the imperial palace in Beijing, China, during the Qing dynasty (1636-1912. Most lanterns had colourful tassels made of fibres. The study performed a comprehensive investigation of the materials and dyes used for palace lantern tassels preserved in The Palace Museum, Beijing. Eight samples with different colours, including yellow, green and red, from five palace lanterns were analysed. By using ultra-performance liquid chromatography combined with time-of-flight mass spectrometry, the compositions of the dyes were identified quickly, and thus the dyes' origins were explored. A Xray fluorescence spectrometer assisted in the detection of whether a mordant was used during the dyeing process. As a result, the tassels were all made of silk, discovered through the use of microscopic observation and micro-Fourier transform infrared spectrometry analysis. Different dyeing techniques were found in these tassels: yellow tassels were dyed by cork tree or turmeric by a direct dyeing method; greens were dyed by pagoda bud with an iron mordant, or by the combination of cork tree and indigo using multi-dyeing method; and red tassels were coloured with different acid red industrial dyes. These results provide valuable data for the conservation of ancient textile tassels and contribute to the investigation of other hanging tags.

“…The developed UPLC-MS/MS was used for the subsequent quantification of all Gelsemium alkaloids, which has a faster analysis time than traditional HPLC [ 35 , 36 ] or LC-MS [ 37 – 39 ] and greatly enhances the signal intensity. It only takes five minutes to analyze a plasma sample, which can save significant time when analyzing hundreds of samples.…”

Section: Discussionmentioning

confidence: 99%

Toxicokinetics of 11 Gelsemium Alkaloids in Rats by UPLC-MS/MS

Shen

BioMed Research International

et al. 2020

Gelsemium elegans (Gardn. & Champ.) Benth. is a plant belonging to the genus Gelsemium (family Gelsemiaceae), and its main components are alkaloids. It is a Chinese traditional medicinal plant and notoriously known as a highly toxic medicine. However, a method has not yet been found for the simultaneous detection of 11 Gelsemium alkaloids in rat plasma, and the toxicokinetics of 11 Gelsemium alkaloids after intravenous administration has not been reported. In this work, we have developed a sensitive and rapid method of ultraperformance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) for the detection of 11 Gelsemium alkaloids in rat plasma. The toxicokinetic behavior was also investigated, so as to provide a reference of the scientific properties of Gelsemium elegans and improve the efficacy and safety of drugs. Sixty-six Sprague-Dawley rats were randomly divided into 11 groups, six rats in each group. Each group was intravenously given one alkaloid (0.1 mg/kg), respectively. A Waters UPLC BEH C18 column (50 mm×2.1 mm, 1.7 μm) was used for chromatographic separation. Methanol and water (containing 0.1% formic acid) were used for the mobile phase with gradient elution. Multiple reactions were monitored, and positive electrospray ionization was used for quantitative analysis. The precision was less than 16%, and the accuracy was between 86.9% and 113.2%. The extraction efficiency was better than 75.8%, and the matrix effects ranged from 88.5% to 107.8%. The calibration curves were in the range of 0.1–200 ng/mL, with a correlation coefficient (R2) greater than 0.995. The UPLC-MS/MS method was successfully applied to the toxicokinetics of 11 Gelsemium alkaloids in rats after intravenous administration (0.1 mg/kg for each alkaloid). The results of the toxicokinetics provide a basis for the pharmacology and toxicology of Gelsemium alkaloids and scientific evidence for the clinical use of Gelsemium alkaloids.