CE and CEC analysis of phytochemicals in herbal medicines

Chen, Xiao‐Jia; Zhao, Jing; Wang, Yi Tao; Huang, Lu; Li, Shao‐Ping

doi:10.1002/elps.201100347

Cited by 25 publications

(20 citation statements)

References 251 publications

(450 reference statements)

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“…[2][3][4][5] These isoavones have been shown to have benecial effects in the treatment of patients with cardiovascular disease 6,7 and diabetes 7 and they have been reported to have antithrombotic and antiallergy effects 8 and antimutagenic, 9,10 antioxidant 11 and estrogenic activities. 16,17 Organic solvents such as methanol and acetonitrile have been used as additives in capillary zone electrophoresis (CZE), although this usually increases the total analysis time as a result of the decrease in electro-osmotic ow (EOF) when organic solvents are applied. 5,[13][14][15] There have been several reports of the use of CE in the analysis of natural products on account of its speed, its high efficiency and the small amounts of sample and solution required.…”

Section: Introductionmentioning

confidence: 99%

Determination of eight isoflavones in Radix Puerariae by capillary zone electrophoresis with an ionic liquid as an additive

Xiao

Wang

et al. 2015

Anal. Methods

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Isoflavones are the main active components in Radix Puerariae, the dry root of Pueraria lobata or P. thomsonii. We developed a simple capillary zone electrophoresis (CZE) method with an ionic liquid (IL) as an additive for the simultaneous determination of eight isoflavonesononin, daidzin, genistin, biochanin A, formononetin, puerarin, genistein and daizeinin Radix Puerariae. The experimental conditions, including the concentration of sodium tetraborate, the pH, the type and concentration of the ILs, the applied voltage and the capillary temperature, were investigated. The eight analytes (detection wavelength 260 nm) were well separated within 9 min using a running buffer of 30 mM sodium tetraborate and 50 mM 1-butyl-3-methylimidazolium tetrafluoroborate as an additive at pH 9.5, with an applied voltage of 18 kV and a capillary temperature of 25 C. The method developed was fully validated (limit of detection, 1.72-4.92 mg mL À1 ; limit of quantification, 3.64-9.84 mg mL À1 ; intra-day precision, 1.1-4.7% RSD; inter-day precision, 2.1-6.6% RSD; and recovery, 93.1-107.5% with 4.0-5.9% RSD) and was successfully applied to the determination of the eight analytes in three Radix Puerariae samples. The results indicated that P. lobata contains more isoflavones than P. thomsonii. CZE with an IL as an additive is a promising method for the analysis of natural products.

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Section: Introductionmentioning

confidence: 99%

Determination of eight isoflavones in Radix Puerariae by capillary zone electrophoresis with an ionic liquid as an additive

Xiao

Wang

et al. 2015

Anal. Methods

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“…2 The well-established benets of citrus products are mainly due to the presence of most important class of secondary plant metabolites, namely avonoids. 5 Naringin (4 0 ,5,7-trihydroxyavanone-7-rhamnoglucoside) is a major avonoid present in grapefruit. (Rutaceae)) has received much attention because of its nutritional and antioxidant properties.…”

Section: Introductionmentioning

confidence: 99%

A MEKC method for naringenin from natural and biological samples

et al. 2015

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The work reported describes the development of a micellar electrokinetic chromatographic (MEKC) method for the determination of naringenin in real samples including grapefruit juice and human blood serum using a PDA detector. The effects of different CE parameters such as concentration and pH of the running buffer, voltage, injection time and concentration of sodium dodecyl sulfate (SDS) were optimized. Under the optimized conditions, naringenin could be well determined within 6 min using 40 mM borate buffer, 40 mM SDS at pH 9.0, at an applied voltage of 25 kV. For the quantitative determination of naringenin (flavonoid aglycone) in grapefruit juice, the naringin (flavonoid glycoside)was hydrolysed and the resulting aglycone was identified and quantified. The calibration curve was linear in the studied concentration range of 0.1 to 50 mg mL À1 (R 2 ¼ 0.995). The detection limit and the limit of quantification were found to be 0.05 and 0.19 mg mL À1 , respectively.

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“…In many reports, high performance liquid chromatography (HPLC) is a good technique which has been used in the determination of compounds in Flos Farfarae [20–22]. Nowadays, Capillary electrophoresis gradually becomes a new technology in quality control of Flos Farfarae and other TCM [23–29]. …”

Section: Introductionmentioning

confidence: 99%

Analysis of Three Compounds in Flos Farfarae by Capillary Electrophoresis with Large-Volume Sample Stacking

Hou

Hao

et al. 2017

International Journal of Analytical Chemistry

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The aim of this study was to develop a method combining an online concentration and high-efficiency capillary electrophoresis separation to analyze and detect three compounds (rutin, hyperoside, and chlorogenic acid) in Flos Farfarae. In order to get good resolution and enrichment, several parameters such as the choice of running buffer, pH and concentration of the running buffer, organic modifier, temperature, and separation voltage were all investigated. The optimized conditions were obtained as follows: the buffer of 40 mM NaH2P04-40 mM Borax-30% v/v methanol (pH 9.0); the sample hydrodynamic injection of up to 4 s at 0.5 psi; 20 kV applied voltage. The diode-array detector was used, and the detection wavelength was 364 nm. Based on peak area, higher levels of selective and sensitive improvements in analysis were observed and about 14-, 26-, and 5-fold enrichment of rutin, hyperoside, and chlorogenic acid were achieved, respectively. This method was successfully applied to determine the three compounds in Flos Farfarae. The linear curve of peak response versus concentration was from 20 to 400 µg/ml, 16.5 to 330 µg/mL, and 25 to 500 µg/mL, respectively. The regression coefficients were 0.9998, 0.9999, and 0.9991, respectively.

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CE and CEC analysis of phytochemicals in herbal medicines

Cited by 25 publications

References 251 publications

Determination of eight isoflavones in Radix Puerariae by capillary zone electrophoresis with an ionic liquid as an additive

Determination of eight isoflavones in Radix Puerariae by capillary zone electrophoresis with an ionic liquid as an additive

A MEKC method for naringenin from natural and biological samples

Analysis of Three Compounds in Flos Farfarae by Capillary Electrophoresis with Large-Volume Sample Stacking

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