Determination of eight isoflavones in Radix Puerariae by capillary zone electrophoresis with an ionic liquid as an additive

Xiao, Wenzhong; Wang, Fengqin; Li, Chunhong; Zhang, Qian; Xia, Zhining; Yang, Feng‐Qing

doi:10.1039/c4ay02374a

Cited by 23 publications

(25 citation statements)

References 27 publications

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“…Radix Puerariae, the dry root of Pueraria lobata, also contains large amounts of isoflavones, including puerarin, daidzin, genistein, and biochanin A. These samples are generally extracted with pure methanol and subjected to an ultrasonic bath for 30 min (Niu and others ; Xiao and others , ).…”

Section: Sample Preparationmentioning

confidence: 99%

“…With the commonly used separation buffers, formononetin and biochanin A, which have very similar chemical structures, could not be separated. Xiao and others () have investigated the use of ionic liquids as additives to improve separation of these isoflavones. They found that the addition of ILs had little effect on the EOF, suggesting that the separation mechanism is most likely to be either hydrophobic interactions between the analytes and the ILs and/or the formation of complexes between the analytes and the IL.…”

Section: Separation and Detection Techniquesmentioning

confidence: 99%

“…Detection in CE is usually performed with UV (Dinelli and others ; Xiao and others ) and electrochemical detection (ED) (Chen and others ). Mass spectrometry (MS) coupled to the EC is a powerful alternative combining the cited advantages presented by the EC, with the selectivity of the analysis and the structural information provided by the MS.…”

Section: Separation and Detection Techniquesmentioning

confidence: 99%

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Analysis of Isoflavones in Foods

Bustamante-Rangel

Delgado-Zamarreño

Pérez‐Martín

et al. 2018

Comp Rev Food Sci Food Safe

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In recent years the nutritional and bioactive properties of foods are being intensively investigated with a view to control, in addition to food quality, their possible influence on human health. Because of this, there is a growing demand for rapid, selective, sensitive, and validated methods for analysis and quantification. Bioactive plant compounds include those with weak estrogenic activity (phytoestrogens), among which are the isoflavones. Some of the beneficial activities that have been attributed to isoflavones are anticarcinogenic activity, the prevention of cardiovascular disease, the improvement of bone health, and antioxidant activity. The objective of this work is to provide an updated review of the methods used in sample preparation and subsequent analysis for the determination of isoflavones in food samples, including both soybean and soy products, as well as other foods with low isoflavone contents. The review focuses on the most common sample preparation techniques used during the last 10 years, including both conventional solvent extraction and other more recent extraction techniques. Separation and detection methods, including current trends in liquid chromatography analysis, such as the use of monolithic columns or ultra-high-pressure liquid chromatography, are also discussed.

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Section: Sample Preparationmentioning

confidence: 99%

Section: Separation and Detection Techniquesmentioning

confidence: 99%

Section: Separation and Detection Techniquesmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Isoflavones in Foods

Bustamante-Rangel

Delgado-Zamarreño

Pérez‐Martín

et al. 2018

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

show abstract

“…HP‐β‐CD was also used to improve the separation of diastereomeric pairs, the separation of catechin, epicatechin, cinchonains Ia, Ib, IIa, and IIb from Trichilia catigua samples on the same run could be realized in less than 12 min by using voltage reduction . In our previous study, a simple CZE method with an IL, BMImBF 4 as an additive was developed for the simultaneous determination of eight isoflavones including ononin, daidzin, genistin, biochanin A, formononetin, puerarin, genistein and daizein in Radix Puerariae . The eight analytes (detection wavelength 260 nm) were well separated within 9 min after systematic optimization of the parameters.…”

Section: Methods Developmentmentioning

confidence: 99%

“…Ohwi or P. thomsonii Benth, contains large amounts of isoflavones. A CZE method with an IL 1‐butyl‐3‐methylimidazolium tetrafluoroborate as additive for the simultaneous determination of eight isoflavones in Radix Puerariae . The results indicated that P. lobata contains more isoflavones than P. thomsonii .…”

Section: Applicationsmentioning

confidence: 99%

Capillary electrophoresis in phytochemical analysis (2014–2017)

Zhou

Zhang

2018

Separation Science Plus

Self Cite

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This updated review is focused on the applications of CE in phytochemical analysis during the period of 2014 to April 2018 (about 130 papers were included). The article mainly consists of three main sections, including sample pretreatment (extraction and on‐line pre‐concentration), method development (separation and detection) and applications in quality evaluation of medicinal plants, screening of active compounds (enzyme inhibitors) and studying interaction between phytochemicals and bio‐macromolecules.

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Quantitative separation of hesperidin, chrysin, epicatechin, epigallocatechin gallate, and morin using ionic liquid as a buffer additive in capillary electrophoresis

et al. 2018

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Recently, an increasing interest has been observed in ionic liquids (ILs) due to their potentialities in various chemical processes. ILs have some unique properties making them excellent additives in CE. In this work a simple, rapid, and reliable CZE method has been developed and validated using 1-butyl-3-methyl imidazolium hexafluorophosphate (BMIM-PF ) ionic liquid as a buffer additive for the determination/separation of five flavonoids including hesperedin, epicatechin (EC), epigallocatechin gallate (EGCG), and morin using photodiode array (PDA) detector. The effect of several parameters such as concentration and pH of the running buffer, applied voltage, and concentration of ionic liquid were optimized. CZE at 25°C with 25 mM borate buffer of pH 9.0 at an applied voltage of 17 kV by adding 17.5 mM of IL was found to be suitable for the separation/determination of all five analytes within 08 min. Validation of the method was performed in terms of linearity, accuracy, precision, and limit of detection and quantification. The calibration curves were plotted in the concentration range of 1-200 μg/mL for all five analytes. The response was linear with R = 0.990 for EC, chrysin, and hesperidin, 0.992 for morin, and 0.988 for EGCG. LOD and LOQ were obtained within the range of 0.4-0.5 and 1.4-1.7 μg/mL, respectively. The proposed method showed good reproducibility with RSD of less than 3% for both migration time and peak height. The method was successfully applied for the determination of flavonoids from citrus fruits and tea samples.

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Determination of eight isoflavones in Radix Puerariae by capillary zone electrophoresis with an ionic liquid as an additive

Cited by 23 publications

References 27 publications

Analysis of Isoflavones in Foods

Analysis of Isoflavones in Foods

Capillary electrophoresis in phytochemical analysis (2014–2017)

Quantitative separation of hesperidin, chrysin, epicatechin, epigallocatechin gallate, and morin using ionic liquid as a buffer additive in capillary electrophoresis

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