A comparative study of specificity of fucoidanases from marine microorganisms and invertebrates

Kusaykin, Mikhail I.; Chizhov, Alexander O.; Грачев, А. А.; Alekseeva, S. A.; Bakunina, I. Yu.; Nedashkovskaya, Olga I.; Sova, V. V.; Zvyagintseva, T. N.

doi:10.1007/978-1-4020-5670-3_18

Cited by 16 publications

(19 citation statements)

References 10 publications

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“…3.2.1.44) can hydrolyze fucoidan to produce sulfated LMWF without the removal of its side substitute groups because of its substrate hydrolysis property. There are only a very few studies on isolation and characterization of fucoidanases [ 10 ]. To date, fucoidanases are reported only from marine organisms such as bacteria [ 11 , 12 , 13 ], fungi [ 9 , 14 , 15 ], and invertebrates [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells

Manivasagan

2015

Marine Drugs

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Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

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Section: Introductionmentioning

confidence: 99%

Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells

Manivasagan

2015

Marine Drugs

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“…Crude fucoidans from the brown seaweed Sargassum mcclurei and from sea cucumbers Stichopus variegatus, Holothuria spinifera were prepared as described by Zvyagintseva et al and after that fucoidans were purified by ion-exchange chromatography [15,16]. The structural characteristics of fucoidans from the brown seaweed Sargassum mcclurei were reported before by our colleagues [17].…”

Section: Methodsmentioning

confidence: 84%

Catalytic Conditions of Fucoidan Degrading Enzymes From Vasticardium Flavum

Khanh¹,

Trang²,

Thinh³

et al. 2019

JST

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The fucoidanase from the digestive glands of the marine shell Vasticardium flavum was studied. The fucoidanase catalysed the hydrolysis of 1→3-L-fucan from sea cucumbers Stichopus variegatus, Holothuria spinifera, did not catalyze the hydrolysis of fucoidan from F. evanescens and F. vesiculosus containing alternating α-1→4 and α-1→3 glycoside bonds. This enzyme also did not catalyze the hydrolysis of fucoidan from U. pinnatifida, S. mcclurei, which belong to the galactofucan group. Optima of pH and time incubation were at 3-4 and 24 hours, respectively. The enzyme activity was significantly increased in the presence of the Ca2+, Ba2+, Co2+ and Mg2+ cations, but the Cu2+, Sn2+, Fe2+ and Al3+ cations partially inactivated the enzyme. The enzyme was completely inactivated after 5 min of incubation at 65° C.

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“…In addition, the study showed that the fucoidanases from both L. kurila and P. citrea KMM 3296 had similar specificity, cleaving, predominantly, ␣-(1 → 3)-glycosidic bonds between fucose residues. On the other hand, the released products were different, with bacterial fucoidanase forming mainly di-, tri-, tetra-, and pentafucooligosaccharides, whereas the basic form of fucoidanase from the mollusk yielded higher molecular weight products from fucoidan (of 3-10 kDa) [8].…”

Section: Hydrolases and Lyasesmentioning

confidence: 98%

“…In despite of the enzymes application in sulfated polysaccharide derivatization, widely used in structural analysis, the search for microorganisms and culture optimization and purification of those enzymes for the studies of polysaccharides biological activities are recent in comparison to the extensive usage of other treatments [8]. The use of microorganisms to produce enzymes that could be applied to SPs modifications present some advantages such as: rapid growth in culture medium constituted by different substrates; production of the enzymes in feasible quantities and conditions; easy manipulation and, if well preserved, microorganisms could be reactivated and reused.…”

Section: Enzymatic Modificationmentioning

confidence: 99%

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“Algae’s sulfated polysaccharides modifications: Potential use of microbial enzymes”

Gurpilhares

Moreira

Bueno

et al. 2016

Process Biochemistry

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Marine algae are valuable sources of structurally diverse bioactive compounds, among them, the group of sulfated polysaccharides (SPs). As an example of SPs, it could be cited the fucoidans in brown algae, carrageenans in red algae and ulvans in green algae. Since the past decades, SPs have been extracted from algae and evaluated in respect to their beneficial biological activities such as anti-inflammatory, antioxidative, antimicrobial, anticoagulant, antithrombotic, immunological and anticancer. This review deals with the presentation of natively bioactive SPs structural features in addition to already employed approaches of SPs structure modifications, such as chemical and physical methods. Moreover, this article presents the advantages and the importance of using enzymatic methods during SPs modification, and particularly, the use of microorganisms as enzymes sources. Enzymes such as hydrolases/glycosidases (e.g. fucoidanase, fucosidase, agarase and carrageenase), lyases, sulfotransferases and sulfatases could be used for this purpose. It has been shown that more studies are necessary to obtain/identify opportunities to create novel, or to intensify, SPs biological properties. Considering the advantages in the use of microbial enzymes in biotransformation processes, studies related to the modification of SPs by microorganisms should be stimulated.

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A comparative study of specificity of fucoidanases from marine microorganisms and invertebrates

Cited by 16 publications

References 10 publications

Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells

Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells

Catalytic Conditions of Fucoidan Degrading Enzymes From Vasticardium Flavum

“Algae’s sulfated polysaccharides modifications: Potential use of microbial enzymes”

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