Catalytic Conditions of Fucoidan Degrading Enzymes From Vasticardium Flavum

Abstract

The fucoidanase from the digestive glands of the marine shell Vasticardium flavum was studied. The fucoidanase catalysed the hydrolysis of 1→3-L-fucan from sea cucumbers Stichopus variegatus, Holothuria spinifera, did not catalyze the hydrolysis of fucoidan from F. evanescens and F. vesiculosus containing alternating α-1→4 and α-1→3 glycoside bonds. This enzyme also did not catalyze the hydrolysis of fucoidan from U. pinnatifida, S. mcclurei, which belong to the galactofucan group. Optima of pH and time incubation were at 3-4 and 24 hours, respectively. The enzyme activity was significantly increased in the presence of the Ca2+, Ba2+, Co2+ and Mg2+ cations, but the Cu2+, Sn2+, Fe2+ and Al3+ cations partially inactivated the enzyme. The enzyme was completely inactivated after 5 min of incubation at 65° C.

A recent update on fucoidonase: source, Isolation methods and its enzymatic activity

“…Ions of Cu 2+ and Pb 2+ produce no effect on the activity of these enzymes, while ions of Co 2+ and Mg 2+ slightly activate the enzymes (Thanassi and Nakada, 1967;Furukawa et al, 1992). The activity of fucoidanase from Vasticardium flavum is enhanced by Ca 2+ , Ba 2+ , Co 2+ , and Mg 2+ cations, but inhibited by Cu 2+ , Sn 2+ , Fe 2+ , and Al 3+ cations (Khanh et al, 2019a). In one study (Nagao et al, 2018), the activity of the enzyme was intense by divalent cations such as Ba 2+ , Fe 2+ , and Ca 2+ , while inhibited by Co 2+ , Cu 2+ , Mn 2+ , and Zn 2+ (Nagao et al, 2018) and Formosa KMM3553 fucoidanase requires metal ions for its activity (Silchenko et al, 2013) (Table 3).…”

“…Haliotus sp. marine mollusk (Thanassi and Nakada, 1967) Mizuhopecten yessoensis (Patinopecten yessoensis) marine mollusk (Kitamura et al, 1992) Vasticardium flavum marine mollusk (Khanh et al, 2019b) Echinodermata echinoderms (Chevolot et al, 2001;Larsson et al, 2003;Bilan et al, 2004;Bilan et al, 2005;Yang et al, 2021b;Abdel-Latif et al, 2022;Ahmad et al, 2022) Strongylocentrotus nudus Sea urchins (Sasaki et al, 1996) biochemical properties were studied. It was found that FFA2 has amino acid sequence identical to the one of fucoidanase FcnA isolated from M. fucanivorans.…”

“…(Thanassi and Nakada, 1967), Lambis sp. (Silchenko et al, 2014), Vasticardium flavum (Khanh et al, 2019a), and echinus Strongylocentrotus nudus (Sasaki et al, 1996), showed supreme activity in the pH range of 3.5-5.5 (Table 2). There are only a few publications so far on marine fungal fucoidanase.…”

“…For marine invertebrate fucoidanase, the optimal temperature range is 38-45°C (Silchenko et al, 2014) (Kusaykin et al, 2003) (Thanassi and Nakada, 1967;Sasaki et al, 1996;Khanh et al, 2019a), and for marine bacteria and fungi it is 37-50°C (Furukawa et al, 1992;Lee et al, 2012;Silchenko et al, 2013;Nagao et al, 2018;Vuillemin et al, 2020) and 50-60°C (Qianqian et al, 2011;Wu et al, 2011). Marine bacterium Vibrio sp.…”